GPA7_DICDI
ID GPA7_DICDI Reviewed; 390 AA.
AC P34045; Q551N2; Q86A65;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 3.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Guanine nucleotide-binding protein alpha-7 subunit;
DE Short=G alpha-7;
GN Name=gpaG; ORFNames=DDB_G0276455;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=AX3;
RX PubMed=7949425; DOI=10.1091/mbc.5.6.691;
RA Wu L., Gaskins C.J., Zhou K., Firtel R.A., Devreotes P.N.;
RT "Cloning and targeted mutations of G alpha 7 and G alpha 8, two
RT developmentally regulated G protein alpha-subunit genes in Dictyostelium.";
RL Mol. Biol. Cell 5:691-702(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 84-237.
RC STRAIN=AX3;
RX PubMed=1930158; DOI=10.1016/0006-291x(91)91690-e;
RA Wu L., Devreotes P.N.;
RT "Dictyostelium transiently expresses eight distinct G-protein alpha-
RT subunits during its developmental program.";
RL Biochem. Biophys. Res. Commun. 179:1141-1147(1991).
CC -!- FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved
CC as modulators or transducers in various transmembrane signaling
CC systems.
CC -!- SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The
CC alpha chain contains the guanine nucleotide binding site.
CC -!- DEVELOPMENTAL STAGE: Expressed primarily at the aggregation stage.
CC -!- SIMILARITY: Belongs to the G-alpha family. {ECO:0000305}.
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DR EMBL; L33847; AAA67423.1; -; mRNA.
DR EMBL; AAFI02000015; EAL69181.1; -; Genomic_DNA.
DR PIR; JH0248; JH0248.
DR RefSeq; XP_643087.1; XM_637995.1.
DR AlphaFoldDB; P34045; -.
DR SMR; P34045; -.
DR STRING; 44689.DDB0185045; -.
DR PaxDb; P34045; -.
DR EnsemblProtists; EAL69181; EAL69181; DDB_G0276455.
DR GeneID; 8620491; -.
DR KEGG; ddi:DDB_G0276455; -.
DR dictyBase; DDB_G0276455; gpaG.
DR eggNOG; KOG0082; Eukaryota.
DR HOGENOM; CLU_014184_6_0_1; -.
DR InParanoid; P34045; -.
DR OMA; EFIVIIY; -.
DR PhylomeDB; P34045; -.
DR PRO; PR:P34045; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005834; C:heterotrimeric G-protein complex; IBA:GO_Central.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central.
DR GO; GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007214; P:gamma-aminobutyric acid signaling pathway; IMP:dictyBase.
DR CDD; cd00066; G-alpha; 1.
DR Gene3D; 1.10.400.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR001019; Gprotein_alpha_su.
DR InterPro; IPR011025; GproteinA_insert.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR10218; PTHR10218; 1.
DR Pfam; PF00503; G-alpha; 1.
DR PRINTS; PR00318; GPROTEINA.
DR SMART; SM00275; G_alpha; 1.
DR SUPFAM; SSF47895; SSF47895; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51882; G_ALPHA; 1.
PE 2: Evidence at transcript level;
KW GTP-binding; Magnesium; Metal-binding; Nucleotide-binding;
KW Reference proteome; Transducer.
FT CHAIN 1..390
FT /note="Guanine nucleotide-binding protein alpha-7 subunit"
FT /id="PRO_0000203665"
FT DOMAIN 70..390
FT /note="G-alpha"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 73..86
FT /note="G1 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 211..219
FT /note="G2 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 234..243
FT /note="G3 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 303..310
FT /note="G4 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 361..366
FT /note="G5 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT BINDING 78..85
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 85
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 213..219
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 238..242
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 307..310
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 363
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT CONFLICT 296
FT /note="W -> L (in Ref. 1; AAA67423)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 390 AA; 44006 MW; 38B7D4CAB87A5C9D CRC64;
MSSTTTNTTT ATPAIQVNGN QSSSPQSPSS STSTLSPPMS PSLLTRYREQ KAVNKQIEKQ
LKEEKKIMDS ELKLLLLGTG DSGKSTVVKQ MKILHLEGYS QEERINQRQF VYRNIIEIAY
SIIRGCGVLN LTIPSQFDSI CSSIEEIYET KNYTNLDKNV LKGVAELSKN ESFINAANNS
GSNFQLHSSS QYFLDDIARF SEEDYIPTDQ DILYTRVAST SVSETRFSVR GIKFRMIDVA
GQRGHRDKWI HHFSEVTAIL FVISLCEYDQ VLEEDGKTNR MIESIKVFGD IINQRWFKDI
PIILFLNKRD LFAEKIKKTG ISICFPDYTG PSDDYEQSLV FLKKKILSAN KTSKAVYTNV
TTATDTTNIG HVFEAVKDIL TRQTMEEGGI
