GPA8_DICDI
ID GPA8_DICDI Reviewed; 403 AA.
AC P34046; Q54PH7;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Guanine nucleotide-binding protein alpha-8 subunit;
DE Short=G alpha-8;
GN Name=gpaH; ORFNames=DDB_G0284469;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=AX3;
RX PubMed=7949425; DOI=10.1091/mbc.5.6.691;
RA Wu L., Gaskins C.J., Zhou K., Firtel R.A., Devreotes P.N.;
RT "Cloning and targeted mutations of G alpha 7 and G alpha 8, two
RT developmentally regulated G protein alpha-subunit genes in Dictyostelium.";
RL Mol. Biol. Cell 5:691-702(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 45-198.
RC STRAIN=AX3;
RX PubMed=1930158; DOI=10.1016/0006-291x(91)91690-e;
RA Wu L., Devreotes P.N.;
RT "Dictyostelium transiently expresses eight distinct G-protein alpha-
RT subunits during its developmental program.";
RL Biochem. Biophys. Res. Commun. 179:1141-1147(1991).
CC -!- FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved
CC as modulators or transducers in various transmembrane signaling
CC systems. G alpha-8 is a potential analog for the G(s)-like G-proteins
CC which stimulate adenylate cyclase in mammals.
CC -!- SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The
CC alpha chain contains the guanine nucleotide binding site.
CC -!- DEVELOPMENTAL STAGE: Expressed primarily at the mound stage.
CC -!- SIMILARITY: Belongs to the G-alpha family. {ECO:0000305}.
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DR EMBL; L33848; AAA67424.1; -; mRNA.
DR EMBL; AAFI02000066; EAL65161.1; -; Genomic_DNA.
DR RefSeq; XP_638555.1; XM_633463.1.
DR AlphaFoldDB; P34046; -.
DR SMR; P34046; -.
DR STRING; 44689.DDB0219991; -.
DR PaxDb; P34046; -.
DR PRIDE; P34046; -.
DR EnsemblProtists; EAL65161; EAL65161; DDB_G0284469.
DR GeneID; 8624648; -.
DR KEGG; ddi:DDB_G0284469; -.
DR dictyBase; DDB_G0284469; gpaH.
DR eggNOG; KOG0082; Eukaryota.
DR HOGENOM; CLU_014184_6_0_1; -.
DR InParanoid; P34046; -.
DR OMA; EEDCIMT; -.
DR PhylomeDB; P34046; -.
DR PRO; PR:P34046; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005834; C:heterotrimeric G-protein complex; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:dictyBase.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central.
DR GO; GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:1903665; P:negative regulation of asexual reproduction; IMP:dictyBase.
DR GO; GO:0022409; P:positive regulation of cell-cell adhesion; IMP:dictyBase.
DR GO; GO:0010811; P:positive regulation of cell-substrate adhesion; IMP:dictyBase.
DR GO; GO:1903669; P:positive regulation of chemorepellent activity; IDA:dictyBase.
DR GO; GO:0043938; P:positive regulation of sporulation; IMP:dictyBase.
DR GO; GO:0045595; P:regulation of cell differentiation; IMP:dictyBase.
DR CDD; cd00066; G-alpha; 1.
DR Gene3D; 1.10.400.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR001019; Gprotein_alpha_su.
DR InterPro; IPR011025; GproteinA_insert.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR10218; PTHR10218; 1.
DR Pfam; PF00503; G-alpha; 1.
DR PRINTS; PR00318; GPROTEINA.
DR SMART; SM00275; G_alpha; 1.
DR SUPFAM; SSF47895; SSF47895; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51882; G_ALPHA; 1.
PE 2: Evidence at transcript level;
KW GTP-binding; Lipoprotein; Magnesium; Metal-binding; Myristate;
KW Nucleotide-binding; Palmitate; Reference proteome; Transducer.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000255"
FT CHAIN 2..403
FT /note="Guanine nucleotide-binding protein alpha-8 subunit"
FT /id="PRO_0000203666"
FT DOMAIN 31..356
FT /note="G-alpha"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 34..47
FT /note="G1 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 172..180
FT /note="G2 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 195..204
FT /note="G3 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 264..271
FT /note="G4 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 322..327
FT /note="G5 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 353..403
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 362..403
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 39..46
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 46
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 174..180
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 180
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 199..203
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 268..271
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 324
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000255"
FT LIPID 3
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT CONFLICT 125..128
FT /note="Missing (in Ref. 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 137
FT /note="K -> E (in Ref. 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 403 AA; 46312 MW; 3D3D8865898B387B CRC64;
MGCYQSRVQV EDKTNEQLDK YLVQAGKEGL LDFRILLLGA GESGKSTVVK QLKSIYKIQV
DDDELHSYAV NIHKNTVLCM QVLLEAGETL GIELTDPETK KRAINVKSFQ FEPEVKQMPV
SIGLDIEELW RDEDIQKIWD RRSEYWFLDA TPYYFENIQR FLDDDFVPTE EDCIMTRVRT
TGISVTEFDE GPVHFRVVDV GGQRNERKKW IHCFDDVKAL LFVVNLAGYD QVMFEDPSQN
RMQESLTLFG QICNNPIFSE TPTFLVLNKK DLFEQMIQKT DLSKCFPDYK GGSDVKTALE
FIQMKYQQKI QESNKPLHTF HIAARYKKDI KYTWEEAKGI LLEENKKVLM KATKDLKKSS
KQSSKSSLGN STQNNSNNNN NNNNSNNNNG QTTIDGATAK INS
