GPAT1_ARATH
ID GPAT1_ARATH Reviewed; 585 AA.
AC Q9SHJ5; Q8L8V5;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Glycerol-3-phosphate acyltransferase 1;
DE Short=AtGPAT1;
DE EC=2.3.1.15;
GN Name=GPAT1; OrderedLocusNames=At1g06520; ORFNames=F12K11.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=12897259; DOI=10.1105/tpc.012427;
RA Zheng Z., Xia Q., Dauk M., Shen W., Selvaraj G., Zou J.;
RT "Arabidopsis AtGPAT1, a member of the membrane-bound glycerol-3-phosphate
RT acyltransferase gene family, is essential for tapetum differentiation and
RT male fertility.";
RL Plant Cell 15:1872-1887(2003).
CC -!- FUNCTION: Esterifies acyl-group from acyl-ACP to the sn-1 position of
CC glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.
CC Involved in pollen development, by being required for tapetum
CC differentiation and male fertility. In addition to the sporophytic
CC effect, it also exerts a gametophytic effect on pollen performance.
CC {ECO:0000269|PubMed:12897259}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an acyl-CoA + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-
CC phosphate + CoA; Xref=Rhea:RHEA:15325, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57597, ChEBI:CHEBI:57970, ChEBI:CHEBI:58342; EC=2.3.1.15;
CC Evidence={ECO:0000269|PubMed:12897259};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC Vmax=217.42 pmol/min/mg enzyme {ECO:0000269|PubMed:12897259};
CC -!- PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-
CC diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:12897259}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:12897259}. Mitochondrion
CC {ECO:0000269|PubMed:12897259}. Note=According to PubMed:12897259 it is
CC mitochondrial. However, no clear transit peptide is predicted by
CC sequence analysis tools.
CC -!- TISSUE SPECIFICITY: Highly expressed in developing siliques and flower
CC buds. Weakly or not expressed in roots, seedlings and leaves.
CC {ECO:0000269|PubMed:12897259}.
CC -!- DOMAIN: The HXXXXD motif is essential for acyltransferase activity and
CC may constitute the binding site for the phosphate moiety of the
CC glycerol-3-phosphate. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Plants display a massive pollen development
CC arrest due to a perturbed degeneration of the tapetum, which is
CC associated with altered endoplasmic reticulum profiles and reduced
CC secretion. Defects correlate with several fatty acid composition
CC changes in flower tissues and seeds. However, no significant change in
CC seed oil content is observed. {ECO:0000269|PubMed:12897259}.
CC -!- SIMILARITY: Belongs to the GPAT/DAPAT family. {ECO:0000305}.
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DR EMBL; AC007592; AAF24816.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28001.1; -; Genomic_DNA.
DR EMBL; AY088785; AAM67097.1; -; mRNA.
DR PIR; G86200; G86200.
DR RefSeq; NP_563768.1; NM_100531.3.
DR AlphaFoldDB; Q9SHJ5; -.
DR STRING; 3702.AT1G06520.1; -.
DR PaxDb; Q9SHJ5; -.
DR PRIDE; Q9SHJ5; -.
DR ProteomicsDB; 248461; -.
DR EnsemblPlants; AT1G06520.1; AT1G06520.1; AT1G06520.
DR GeneID; 837163; -.
DR Gramene; AT1G06520.1; AT1G06520.1; AT1G06520.
DR KEGG; ath:AT1G06520; -.
DR Araport; AT1G06520; -.
DR TAIR; locus:2009225; AT1G06520.
DR eggNOG; ENOG502QWBX; Eukaryota.
DR HOGENOM; CLU_028504_1_0_1; -.
DR InParanoid; Q9SHJ5; -.
DR OMA; QDGETMQ; -.
DR OrthoDB; 423986at2759; -.
DR PhylomeDB; Q9SHJ5; -.
DR BRENDA; 2.3.1.15; 399.
DR UniPathway; UPA00557; UER00612.
DR PRO; PR:Q9SHJ5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SHJ5; baseline and differential.
DR Genevisible; Q9SHJ5; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0090447; F:glycerol-3-phosphate 2-O-acyltransferase activity; IDA:TAIR.
DR GO; GO:0004366; F:glycerol-3-phosphate O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016791; F:phosphatase activity; IBA:GO_Central.
DR GO; GO:0102420; F:sn-1-glycerol-3-phosphate C16:0-DCA-CoA acyl transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016024; P:CDP-diacylglycerol biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0010143; P:cutin biosynthetic process; IBA:GO_Central.
DR GO; GO:0048235; P:pollen sperm cell differentiation; IMP:TAIR.
DR InterPro; IPR002123; Plipid/glycerol_acylTrfase.
DR Pfam; PF01553; Acyltransferase; 1.
DR SMART; SM00563; PlsC; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Lipid biosynthesis; Lipid metabolism; Membrane;
KW Mitochondrion; Phospholipid biosynthesis; Phospholipid metabolism;
KW Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..585
FT /note="Glycerol-3-phosphate acyltransferase 1"
FT /id="PRO_0000195249"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 356..376
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOTIF 403..408
FT /note="HXXXXD motif"
FT CONFLICT 21
FT /note="K -> N (in Ref. 3; AAM67097)"
FT /evidence="ECO:0000305"
FT CONFLICT 270
FT /note="F -> L (in Ref. 3; AAM67097)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 585 AA; 66514 MW; 3F7FDCE161C28EAF CRC64;
MVLPELLVIL AEWVLYRLLA KSCYRAARKL RGYGFQLKNL LSLSKTQSLH NNSQHHLHNH
HQQNHPNQTL QDSLDPLFPS LTKYQELLLD KNRACSVSSD HYRDTFFCDI DGVLLRQHSS
KHFHTFFPYF MLVAFEGGSI IRAILLLLSC SFLWTLQQET KLRVLSFITF SGLRVKDMDN
VSRSVLPKFF LENLNIQVYD IWARTEYSKV VFTSLPQVLV ERFLREHLNA DDVIGTKLQE
IKVMGRKFYT GLASGSGFVL KHKSAEDYFF DSKKKPALGI GSSSSPQDHI FISICKEAYF
WNEEESMSKN NALPRERYPK PLIFHDGRLA FLPTPLATLA MFIWLPIGFL LAVFRISVGV
FLPYHVANFL ASMSGVRITF KTHNLNNGRP EKGNSGVLYV CNHRTLLDPV FLTTSLGKPL
TAVTYSLSKF SEFIAPLKTV SLKRDRKKDG EAMQRLLSKG DLVVCPEGTT CREPYLLRFS
PLFAELTEDI VPVAVDARVS MFYGTTASGL KCLDPIFFLM NPRPVYCLEI LKKLPKEMTC
AGGKSSFEVA NFIQGELARV LGFECTNLTR RDKYLVLAGN EGIVR
