GPC4_MOUSE
ID GPC4_MOUSE Reviewed; 557 AA.
AC P51655; Q923C8;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2003, sequence version 2.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Glypican-4;
DE AltName: Full=K-glypican;
DE Contains:
DE RecName: Full=Secreted glypican-4;
DE Flags: Precursor;
GN Name=Gpc4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ;
RX PubMed=7657705; DOI=10.1083/jcb.130.5.1207;
RA Watanabe K., Yamada H., Yamaguchi Y.;
RT "K-glypican: a novel GPI-anchored heparan sulfate proteoglycan that is
RT highly expressed in developing brain and kidney.";
RL J. Cell Biol. 130:1207-1218(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, and Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=30982611; DOI=10.1016/j.ajhg.2019.02.026;
RA Amor D.J., Stephenson S.E.M., Mustapha M., Mensah M.A., Ockeloen C.W.,
RA Lee W.S., Tankard R.M., Phelan D.G., Shinawi M., de Brouwer A.P.M.,
RA Pfundt R., Dowling C., Toler T.L., Sutton V.R., Agolini E., Rinelli M.,
RA Capolino R., Martinelli D., Zampino G., Dumic M., Reardon W.,
RA Shaw-Smith C., Leventer R.J., Delatycki M.B., Kleefstra T., Mundlos S.,
RA Mortier G., Bahlo M., Allen N.J., Lockhart P.J.;
RT "Pathogenic variants in GPC4 cause Keipert Syndrome.";
RL Am. J. Hum. Genet. 104:914-924(2019).
CC -!- FUNCTION: Cell surface proteoglycan that bears heparan sulfate. May be
CC involved in the development of kidney tubules and of the central
CC nervous system.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor;
CC Extracellular side.
CC -!- SUBCELLULAR LOCATION: [Secreted glypican-4]: Secreted, extracellular
CC space {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Highly expressed in developing brain and kidney.
CC -!- DISRUPTION PHENOTYPE: Knockout mice have short fore and hind paws, and
CC display a significant decrease in the length of the snout.
CC {ECO:0000269|PubMed:30982611}.
CC -!- SIMILARITY: Belongs to the glypican family. {ECO:0000305}.
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DR EMBL; X83577; CAA58560.1; -; mRNA.
DR EMBL; BC006622; AAH06622.1; -; mRNA.
DR CCDS; CCDS30124.1; -.
DR PIR; A57050; A57050.
DR RefSeq; NP_032176.2; NM_008150.2.
DR AlphaFoldDB; P51655; -.
DR SMR; P51655; -.
DR BioGRID; 200014; 3.
DR IntAct; P51655; 1.
DR MINT; P51655; -.
DR STRING; 10090.ENSMUSP00000033450; -.
DR GlyGen; P51655; 4 sites.
DR iPTMnet; P51655; -.
DR PhosphoSitePlus; P51655; -.
DR SwissPalm; P51655; -.
DR MaxQB; P51655; -.
DR PaxDb; P51655; -.
DR PeptideAtlas; P51655; -.
DR PRIDE; P51655; -.
DR ProteomicsDB; 271315; -.
DR Antibodypedia; 30267; 421 antibodies from 30 providers.
DR DNASU; 14735; -.
DR Ensembl; ENSMUST00000033450; ENSMUSP00000033450; ENSMUSG00000031119.
DR GeneID; 14735; -.
DR KEGG; mmu:14735; -.
DR UCSC; uc012hhb.1; mouse.
DR CTD; 2239; -.
DR MGI; MGI:104902; Gpc4.
DR VEuPathDB; HostDB:ENSMUSG00000031119; -.
DR eggNOG; KOG3821; Eukaryota.
DR GeneTree; ENSGT01050000244897; -.
DR HOGENOM; CLU_024658_2_0_1; -.
DR InParanoid; P51655; -.
DR OMA; LKNCNEV; -.
DR OrthoDB; 611422at2759; -.
DR PhylomeDB; P51655; -.
DR TreeFam; TF105317; -.
DR Reactome; R-MMU-1971475; A tetrasaccharide linker sequence is required for GAG synthesis.
DR Reactome; R-MMU-2022928; HS-GAG biosynthesis.
DR Reactome; R-MMU-2024096; HS-GAG degradation.
DR Reactome; R-MMU-975634; Retinoid metabolism and transport.
DR BioGRID-ORCS; 14735; 2 hits in 75 CRISPR screens.
DR ChiTaRS; Gpc4; mouse.
DR PRO; PR:P51655; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; P51655; protein.
DR Bgee; ENSMUSG00000031119; Expressed in metanephric proximal tubule and 273 other tissues.
DR Genevisible; P51655; MM.
DR GO; GO:0099026; C:anchored component of presynaptic membrane; ISO:MGI.
DR GO; GO:0009986; C:cell surface; IBA:GO_Central.
DR GO; GO:0062023; C:collagen-containing extracellular matrix; HDA:BHF-UCL.
DR GO; GO:0009897; C:external side of plasma membrane; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; HDA:BHF-UCL.
DR GO; GO:0098978; C:glutamatergic synapse; IDA:SynGO.
DR GO; GO:0005796; C:Golgi lumen; TAS:Reactome.
DR GO; GO:0045202; C:synapse; IDA:SynGO.
DR GO; GO:0016477; P:cell migration; IBA:GO_Central.
DR GO; GO:0098696; P:regulation of neurotransmitter receptor localization to postsynaptic specialization membrane; ISO:MGI.
DR GO; GO:1905606; P:regulation of presynapse assembly; ISO:MGI.
DR GO; GO:1905475; P:regulation of protein localization to membrane; IBA:GO_Central.
DR GO; GO:0009966; P:regulation of signal transduction; IEA:InterPro.
DR GO; GO:0099560; P:synaptic membrane adhesion; IDA:SynGO.
DR GO; GO:0016055; P:Wnt signaling pathway; ISO:MGI.
DR InterPro; IPR001863; Glypican.
DR InterPro; IPR031180; Glypican-4.
DR InterPro; IPR019803; Glypican_CS.
DR PANTHER; PTHR10822; PTHR10822; 1.
DR PANTHER; PTHR10822:SF25; PTHR10822:SF25; 1.
DR Pfam; PF01153; Glypican; 1.
DR PROSITE; PS01207; GLYPICAN; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; GPI-anchor; Heparan sulfate; Lipoprotein;
KW Membrane; Phosphoprotein; Proteoglycan; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..529
FT /note="Glypican-4"
FT /id="PRO_0000012317"
FT CHAIN 19..?
FT /note="Secreted glypican-4"
FT /id="PRO_0000333848"
FT PROPEP 530..557
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000012318"
FT MOD_RES 357
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75487"
FT LIPID 529
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 494
FT /note="O-linked (Xyl...) (glycosaminoglycan) serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 498
FT /note="O-linked (Xyl...) (glycosaminoglycan) serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 500
FT /note="O-linked (Xyl...) (glycosaminoglycan) serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 514
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 537
FT /note="A -> T (in Ref. 1; CAA58560)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 557 AA; 62586 MW; F216AA0FEF5ED840 CRC64;
MARLGLLALL CTLAALSASL LAAELKSKSC SEVRRLYVSK GFNKNDAPLY EINGDHLKIC
PQDYTCCSQE MEEKYSLQSK DDFKTVVSEQ CNHLQAIFAS RYKKFDEFFK ELLENAEKSL
NDMFVKTYGH LYMQNSELFK DLFVELKRYY VAGNVNLEEM LNDFWARLLE RMFRLVNSQY
HFTDEYLECV SKYTEQLKPF GDVPRKLKLQ VTRAFVAART FAQGLAVARD VVSKVSVVNP
TAQCTHALLK MIYCSHCRGL VTVKPCYNYC SNIMRGCLAN QGDLDFEWNN FIDAMLMVAE
RLEGPFNIES VMDPIDVKIS DAIMNMQDNS VQVSQKVFQG CGPPKPLPAG RISRSISESA
FSARFRPYHP EQRPTTAAGT SLDRLVTDVK EKLKQAKKFW SSLPSTVCND ERMAAGNENE
DDCWNGKGKS RYLFAVTGNG LANQGNNPEV QVDTSKPDIL ILRQIMALRV MTSKMKNAYN
GNDVDFFDIS DESSGEGSGS GCEYQQCPSE FEYNATDHSG KSANEKADSA GGAHAEAKPY
LLAALCILFL AVQGEWR
