GPC5B_HUMAN
ID GPC5B_HUMAN Reviewed; 403 AA.
AC Q9NZH0; D2DFB0; O75205; Q8NBZ8;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 2.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=G-protein coupled receptor family C group 5 member B;
DE AltName: Full=A-69G12.1;
DE AltName: Full=Retinoic acid-induced gene 2 protein;
DE Short=RAIG-2;
DE Flags: Precursor;
GN Name=GPRC5B; Synonyms=RAIG2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RX PubMed=10783259; DOI=10.1006/geno.2000.6164;
RA Braeuner-Osborne H., Krogsgaard-Larsen P.;
RT "Sequence and expression pattern of a novel human orphan G-protein-coupled
RT receptor, GPRC5B, a family C receptor with a short amino-terminal domain.";
RL Genomics 65:121-128(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR
RP LOCATION, AND INDUCTION.
RX PubMed=10945465; DOI=10.1006/geno.2000.6226;
RA Robbins M.J., Michalovich D., Hill J., Calver A.R., Medhurst A.D.,
RA Gloger I., Sims M.A., Middlemiss D.N., Pangalos M.N.;
RT "Molecular cloning and characterization of two novel retinoic acid-
RT inducible orphan G-protein-coupled receptors (GPRC5B and GPRC5C).";
RL Genomics 67:8-18(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RA Wei H., Osborne B., Spruyt M., Murphy D.;
RT "Cloning of a novel G protein-coupled receptor localized on human
RT chromosome 16p12.";
RL Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RA Cool B.H., Chan G.C.K., Lee L., Oshima J., Martin G.M., Hu Q.;
RT "C-terminal splice variants of Gprc5b, an orphan G protein-coupled receptor
RT that binds Frizzled Wnt receptors, are expressed in maturing neurons and
RT influence neurite outgrowth.";
RL Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10493829; DOI=10.1006/geno.1999.5927;
RA Loftus B.J., Kim U.-J., Sneddon V.P., Kalush F., Brandon R., Fuhrmann J.,
RA Mason T., Crosby M.L., Barnstead M., Cronin L., Mays A.D., Cao Y., Xu R.X.,
RA Kang H.-L., Mitchell S., Eichler E.E., Harris P.C., Venter J.C.,
RA Adams M.D.;
RT "Genome duplications and other features in 12 Mb of DNA sequence from human
RT chromosome 16p and 16q.";
RL Genomics 60:295-308(1999).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15616553; DOI=10.1038/nature03187;
RA Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G.,
RA Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E.,
RA Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J., Buckingham J.M.,
RA Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C.,
RA Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M.,
RA Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M.,
RA Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D.,
RA Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L.,
RA Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E.,
RA Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H.,
RA Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y.,
RA Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S.,
RA Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A.,
RA Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M.,
RA Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H.,
RA Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A.,
RA Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J.,
RA DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J.,
RA Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M.,
RA Myers R.M., Rubin E.M., Pennacchio L.A.;
RT "The sequence and analysis of duplication-rich human chromosome 16.";
RL Nature 432:988-994(2004).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Unknown. This retinoic acid-inducible G-protein coupled
CC receptor provide evidence for a possible interaction between retinoid
CC and G-protein signaling pathways.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:10945465};
CC Multi-pass membrane protein {ECO:0000269|PubMed:10945465}. Cytoplasmic
CC vesicle membrane {ECO:0000269|PubMed:10945465}; Multi-pass membrane
CC protein {ECO:0000269|PubMed:10945465}. Note=Localized in the plasma
CC membrane and perinuclear vesicles.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9NZH0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9NZH0-2; Sequence=VSP_047585;
CC -!- TISSUE SPECIFICITY: Expression is high in kidney, pancreas, and testis,
CC medium in brain, heart, prostate, small intestine, and spleen, low in
CC liver, placenta, skeletal muscle, colon, ovary, and thymus, and not
CC detectable in lung and peripheral leukocyte. According to
CC PubMed:10945465, highly expressed in most brain areas examined, with
CC the highest levels observed in corpus callosum, caudate nucleus,
CC putamen, substantia nigra, thalamus, hippocampus, and spinal cord as
CC well as in dorsal root ganglia (DRG). In the periphery, expression
CC levels are relatively low, compared to the CNS, with the strongest
CC expression detected in pancreas, testis, uterus, and stomach.
CC {ECO:0000269|PubMed:10783259, ECO:0000269|PubMed:10945465}.
CC -!- INDUCTION: By all-trans retinoic acid (ATRA).
CC {ECO:0000269|PubMed:10945465}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 3 family.
CC {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-9 is the initiator.
CC {ECO:0000305}.
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DR EMBL; AF202640; AAF67321.1; -; mRNA.
DR EMBL; AJ276101; CAC00632.1; -; mRNA.
DR EMBL; AF181862; AAF05331.1; -; mRNA.
DR EMBL; FJ529380; ACU30030.1; -; mRNA.
DR EMBL; AK075119; BAC11414.1; -; mRNA.
DR EMBL; AC004131; AAC27544.1; -; Genomic_DNA.
DR EMBL; AC134300; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC034467; AAH34467.1; -; mRNA.
DR CCDS; CCDS10581.1; -. [Q9NZH0-1]
DR RefSeq; NP_057319.1; NM_016235.2. [Q9NZH0-1]
DR RefSeq; XP_006721114.1; XM_006721051.2. [Q9NZH0-1]
DR RefSeq; XP_006721115.1; XM_006721052.2. [Q9NZH0-1]
DR AlphaFoldDB; Q9NZH0; -.
DR SMR; Q9NZH0; -.
DR BioGRID; 119688; 117.
DR IntAct; Q9NZH0; 24.
DR MINT; Q9NZH0; -.
DR STRING; 9606.ENSP00000300571; -.
DR ChEMBL; CHEMBL4523926; -.
DR GlyGen; Q9NZH0; 1 site.
DR iPTMnet; Q9NZH0; -.
DR PhosphoSitePlus; Q9NZH0; -.
DR BioMuta; GPRC5B; -.
DR DMDM; 46396016; -.
DR EPD; Q9NZH0; -.
DR jPOST; Q9NZH0; -.
DR MassIVE; Q9NZH0; -.
DR MaxQB; Q9NZH0; -.
DR PaxDb; Q9NZH0; -.
DR PeptideAtlas; Q9NZH0; -.
DR PRIDE; Q9NZH0; -.
DR ProteomicsDB; 12732; -.
DR ProteomicsDB; 83386; -. [Q9NZH0-1]
DR Antibodypedia; 12141; 310 antibodies from 31 providers.
DR DNASU; 51704; -.
DR Ensembl; ENST00000300571.7; ENSP00000300571.2; ENSG00000167191.12. [Q9NZH0-1]
DR Ensembl; ENST00000535671.5; ENSP00000442858.1; ENSG00000167191.12. [Q9NZH0-2]
DR Ensembl; ENST00000569479.5; ENSP00000454727.1; ENSG00000167191.12. [Q9NZH0-1]
DR Ensembl; ENST00000569847.1; ENSP00000457283.1; ENSG00000167191.12. [Q9NZH0-1]
DR GeneID; 51704; -.
DR KEGG; hsa:51704; -.
DR MANE-Select; ENST00000300571.7; ENSP00000300571.2; NM_016235.3; NP_057319.1.
DR UCSC; uc002dgt.4; human. [Q9NZH0-1]
DR CTD; 51704; -.
DR DisGeNET; 51704; -.
DR GeneCards; GPRC5B; -.
DR HGNC; HGNC:13308; GPRC5B.
DR HPA; ENSG00000167191; Tissue enriched (brain).
DR MIM; 605948; gene.
DR neXtProt; NX_Q9NZH0; -.
DR OpenTargets; ENSG00000167191; -.
DR PharmGKB; PA28938; -.
DR VEuPathDB; HostDB:ENSG00000167191; -.
DR eggNOG; ENOG502QWT9; Eukaryota.
DR GeneTree; ENSGT00950000182961; -.
DR HOGENOM; CLU_044162_1_1_1; -.
DR InParanoid; Q9NZH0; -.
DR OMA; RDNKLAC; -.
DR TreeFam; TF321410; -.
DR PathwayCommons; Q9NZH0; -.
DR SignaLink; Q9NZH0; -.
DR BioGRID-ORCS; 51704; 15 hits in 1086 CRISPR screens.
DR ChiTaRS; GPRC5B; human.
DR GeneWiki; GPRC5B; -.
DR GenomeRNAi; 51704; -.
DR Pharos; Q9NZH0; Tbio.
DR PRO; PR:Q9NZH0; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q9NZH0; protein.
DR Bgee; ENSG00000167191; Expressed in medial globus pallidus and 192 other tissues.
DR ExpressionAtlas; Q9NZH0; baseline and differential.
DR Genevisible; Q9NZH0; HS.
DR GO; GO:0009986; C:cell surface; ISS:CAFA.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0070062; C:extracellular exosome; IDA:UniProtKB.
DR GO; GO:0005615; C:extracellular space; HDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
DR GO; GO:0005730; C:nucleolus; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0043235; C:receptor complex; IBA:GO_Central.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0001664; F:G protein-coupled receptor binding; ISS:CAFA.
DR GO; GO:0030295; F:protein kinase activator activity; IDA:MGI.
DR GO; GO:0019901; F:protein kinase binding; ISS:CAFA.
DR GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; ISS:CAFA.
DR GO; GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB signaling; ISS:CAFA.
DR GO; GO:0050729; P:positive regulation of inflammatory response; ISS:CAFA.
DR GO; GO:0060907; P:positive regulation of macrophage cytokine production; ISS:CAFA.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; ISS:CAFA.
DR GO; GO:0061098; P:positive regulation of protein tyrosine kinase activity; ISS:CAFA.
DR InterPro; IPR017978; GPCR_3_C.
DR Pfam; PF00003; 7tm_3; 1.
DR PROSITE; PS50259; G_PROTEIN_RECEP_F3_4; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Cytoplasmic vesicle;
KW G-protein coupled receptor; Glycoprotein; Membrane; Phosphoprotein;
KW Receptor; Reference proteome; Signal; Transducer; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..403
FT /note="G-protein coupled receptor family C group 5 member
FT B"
FT /id="PRO_0000012965"
FT TOPO_DOM 29..56
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..77
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 78..94
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 116..126
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 148..162
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..199
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 221..234
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 235..255
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 256..271
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..292
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 293..403
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 349..371
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 354
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q923Z0"
FT CARBOHYD 30
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 390..403
FT /note="IPTAPPSHTGRHLW -> EMVTHPRSLESFGAF (in isoform 2)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_047585"
FT CONFLICT 46
FT /note="V -> A (in Ref. 5; BAC11414)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 403 AA; 44795 MW; 3902A16C4F69C26E CRC64;
MFVASERKMR AHQVLTFLLL FVITSVASEN ASTSRGCGLD LLPQYVSLCD LDAIWGIVVE
AVAGAGALIT LLLMLILLVR LPFIKEKEKK SPVGLHFLFL LGTLGLFGLT FAFIIQEDET
ICSVRRFLWG VLFALCFSCL LSQAWRVRRL VRHGTGPAGW QLVGLALCLM LVQVIIAVEW
LVLTVLRDTR PACAYEPMDF VMALIYDMVL LVVTLGLALF TLCGKFKRWK LNGAFLLITA
FLSVLIWVAW MTMYLFGNVK LQQGDAWNDP TLAITLAASG WVFVIFHAIP EIHCTLLPAL
QENTPNYFDT SQPRMRETAF EEDVQLPRAY MENKAFSMDE HNAALRTAGF PNGSLGKRPS
GSLGKRPSAP FRSNVYQPTE MAVVLNGGTI PTAPPSHTGR HLW
