GPC5_MOUSE
ID GPC5_MOUSE Reviewed; 572 AA.
AC Q8CAL5; Q14BN1;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Glypican-5;
DE Contains:
DE RecName: Full=Secreted glypican-5;
DE Flags: Precursor;
GN Name=Gpc5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Hypothalamus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Cell surface proteoglycan that bears heparan sulfate.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Lipid-anchor, GPI-
CC anchor {ECO:0000250}; Extracellular side {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Secreted glypican-5]: Secreted, extracellular
CC space {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glypican family. {ECO:0000305}.
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DR EMBL; AK038547; BAC30037.1; -; mRNA.
DR EMBL; BC115712; AAI15713.1; -; mRNA.
DR CCDS; CCDS27329.1; -.
DR RefSeq; NP_780709.1; NM_175500.4.
DR AlphaFoldDB; Q8CAL5; -.
DR SMR; Q8CAL5; -.
DR BioGRID; 222253; 1.
DR STRING; 10090.ENSMUSP00000135085; -.
DR GlyGen; Q8CAL5; 8 sites.
DR iPTMnet; Q8CAL5; -.
DR PhosphoSitePlus; Q8CAL5; -.
DR MaxQB; Q8CAL5; -.
DR PaxDb; Q8CAL5; -.
DR PRIDE; Q8CAL5; -.
DR ProteomicsDB; 271035; -.
DR Antibodypedia; 24761; 266 antibodies from 33 providers.
DR DNASU; 103978; -.
DR Ensembl; ENSMUST00000022707; ENSMUSP00000022707; ENSMUSG00000022112.
DR GeneID; 103978; -.
DR KEGG; mmu:103978; -.
DR UCSC; uc007uyi.2; mouse.
DR CTD; 2262; -.
DR MGI; MGI:1194894; Gpc5.
DR VEuPathDB; HostDB:ENSMUSG00000022112; -.
DR eggNOG; KOG3821; Eukaryota.
DR GeneTree; ENSGT01050000244955; -.
DR HOGENOM; CLU_024658_4_1_1; -.
DR InParanoid; Q8CAL5; -.
DR OMA; PKPDKWE; -.
DR OrthoDB; 1097767at2759; -.
DR PhylomeDB; Q8CAL5; -.
DR Reactome; R-MMU-1971475; A tetrasaccharide linker sequence is required for GAG synthesis.
DR Reactome; R-MMU-2022928; HS-GAG biosynthesis.
DR Reactome; R-MMU-2024096; HS-GAG degradation.
DR Reactome; R-MMU-5362798; Release of Hh-Np from the secreting cell.
DR Reactome; R-MMU-975634; Retinoid metabolism and transport.
DR BioGRID-ORCS; 103978; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Gpc5; mouse.
DR PRO; PR:Q8CAL5; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q8CAL5; protein.
DR Bgee; ENSMUSG00000022112; Expressed in substantia nigra and 93 other tissues.
DR ExpressionAtlas; Q8CAL5; baseline and differential.
DR Genevisible; Q8CAL5; MM.
DR GO; GO:0046658; C:anchored component of plasma membrane; IEA:InterPro.
DR GO; GO:0009986; C:cell surface; IBA:GO_Central.
DR GO; GO:0062023; C:collagen-containing extracellular matrix; IEA:InterPro.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0005796; C:Golgi lumen; TAS:Reactome.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:MGI.
DR GO; GO:0016477; P:cell migration; IBA:GO_Central.
DR GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; IBA:GO_Central.
DR GO; GO:1905475; P:regulation of protein localization to membrane; IBA:GO_Central.
DR InterPro; IPR001863; Glypican.
DR InterPro; IPR031188; Glypican-5.
DR InterPro; IPR019803; Glypican_CS.
DR PANTHER; PTHR10822; PTHR10822; 1.
DR PANTHER; PTHR10822:SF12; PTHR10822:SF12; 1.
DR Pfam; PF01153; Glypican; 1.
DR PROSITE; PS01207; GLYPICAN; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; GPI-anchor; Heparan sulfate; Lipoprotein;
KW Membrane; Proteoglycan; Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..?
FT /note="Glypican-5"
FT /id="PRO_0000012321"
FT CHAIN 25..?
FT /note="Secreted glypican-5"
FT /id="PRO_0000333850"
FT PROPEP ?..572
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000012322"
FT CARBOHYD 120
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 237
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 486
FT /note="O-linked (Xyl...) (glycosaminoglycan) serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 493
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 495
FT /note="O-linked (Xyl...) (glycosaminoglycan) serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 507
FT /note="O-linked (Xyl...) (glycosaminoglycan) serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 509
FT /note="O-linked (Xyl...) (glycosaminoglycan) serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 527
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 572 AA; 63792 MW; 1935E2B9B77ECB61 CRC64;
MDARTWRLGW RCLLLLALLG STRSEGVESC EEVRKLFQWR LGGAVKGLPE APRAGPDLQV
CLSKNPTCCT RKMEERYQIA ARQDLQQVLQ TSSSTLKLLI SRNAAAFQET LETLIRQAEN
YTSILFCNTY RNMALEAAAS IQEFFTDVGL YLFGADVNPE EFVNRFFDSL FPLVYNHLIN
PGVTDSSLQY SECIRMARQD VSPFGNIPKR VMGQMGRSLL PGRTFLQALN LGIEVINTTD
HIHFSKECSR ALLKMQYCPH CQSLMLSKPC MGYCLNVIRG CLAHMTELNP HWHAYIRSLE
ELSDAMHGTY DVEHVLLNFH LLVNDAVLQA HLNGQKLLDQ VNTICGHPVR TPTQSPRCTF
DPSKEKHGMK ISARNGEETL ANRRKEFINS LRLHGSFYGG LADQLCVNEL AAPEGRPCWN
GEEIVKSYAQ RVVGNGIKAQ SANPEVRVRG TDPVVNQIID KLKHVIQLLR GRSPKPNKWE
LLQPGSGGGM LENSSGDCDD EDGCGGSGSG EVKRTLKITN WMPDSMNFSD VKQVHRADHG
STLDTTSTGC ASGTESMALP LMGTLMFLPW LW
