GPC6A_RAT
ID GPC6A_RAT Reviewed; 928 AA.
AC Q70VB1;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=G-protein coupled receptor family C group 6 member A;
DE Flags: Precursor;
GN Name=Gprc6a;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR
RP LOCATION, AND GLYCOSYLATION.
RC STRAIN=Wistar; TISSUE=Circumvallate papilla;
RX PubMed=17478059; DOI=10.1016/j.gene.2007.03.008;
RA Wellendorph P., Burhenne N., Christiansen B., Walter B., Schmale H.,
RA Brauner-Osborne H.;
RT "The rat GPRC6A: cloning and characterization.";
RL Gene 396:257-267(2007).
CC -!- FUNCTION: Receptor activated by amino acids with a preference for basic
CC amino acids such as L-Lys, L-Arg and L-ornithine but also by small and
CC polar amino acids. The L-alpha amino acids respond is augmented by
CC divalent cations Ca(2+) and Mg(2+). Activated by extracellular calcium
CC and osteocalcin. Seems to act through a G(q)/G(11) and G(i)-coupled
CC pathway. Mediates the non-genomic effects of androgens in multiple
CC tissue. May coordinate nutritional and hormonal anabolic signals
CC through the sensing of extracellular amino acids, osteocalcin, divalent
CC ions and its responsiveness to anabolic steroids (By similarity).
CC {ECO:0000250, ECO:0000269|PubMed:17478059}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:17478059};
CC Multi-pass membrane protein {ECO:0000269|PubMed:17478059}.
CC -!- TISSUE SPECIFICITY: High expression in soft palate. Weak expression in
CC kidney, liver, lung and brain. No expression detected in heart, testis,
CC skeletal muscle amd spleen. {ECO:0000269|PubMed:17478059}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:17478059}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 3 family.
CC {ECO:0000305}.
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DR EMBL; AJ535460; CAD59483.1; -; mRNA.
DR RefSeq; NP_001258035.1; NM_001271106.1.
DR AlphaFoldDB; Q70VB1; -.
DR SMR; Q70VB1; -.
DR MINT; Q70VB1; -.
DR STRING; 10116.ENSRNOP00000000451; -.
DR GlyGen; Q70VB1; 2 sites.
DR PhosphoSitePlus; Q70VB1; -.
DR PaxDb; Q70VB1; -.
DR PRIDE; Q70VB1; -.
DR Ensembl; ENSRNOT00000000451; ENSRNOP00000000451; ENSRNOG00000000401.
DR GeneID; 294394; -.
DR KEGG; rno:294394; -.
DR UCSC; RGD:735077; rat.
DR CTD; 222545; -.
DR RGD; 735077; Gprc6a.
DR eggNOG; KOG1056; Eukaryota.
DR GeneTree; ENSGT00940000158416; -.
DR HOGENOM; CLU_005389_1_0_1; -.
DR InParanoid; Q70VB1; -.
DR OMA; VFIITDQ; -.
DR OrthoDB; 119538at2759; -.
DR PhylomeDB; Q70VB1; -.
DR TreeFam; TF331269; -.
DR Reactome; R-RNO-416476; G alpha (q) signalling events.
DR Reactome; R-RNO-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR PRO; PR:Q70VB1; -.
DR Proteomes; UP000002494; Chromosome 20.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0043200; P:response to amino acid; ISO:RGD.
DR Gene3D; 2.10.50.30; -; 1.
DR InterPro; IPR001828; ANF_lig-bd_rcpt.
DR InterPro; IPR000337; GPCR_3.
DR InterPro; IPR011500; GPCR_3_9-Cys_dom.
DR InterPro; IPR038550; GPCR_3_9-Cys_sf.
DR InterPro; IPR017978; GPCR_3_C.
DR InterPro; IPR000068; GPCR_3_Ca_sens_rcpt-rel.
DR InterPro; IPR017979; GPCR_3_CS.
DR InterPro; IPR028082; Peripla_BP_I.
DR PANTHER; PTHR24061; PTHR24061; 1.
DR Pfam; PF00003; 7tm_3; 1.
DR Pfam; PF01094; ANF_receptor; 1.
DR Pfam; PF07562; NCD3G; 1.
DR PRINTS; PR00592; CASENSINGR.
DR PRINTS; PR00248; GPCRMGR.
DR SUPFAM; SSF53822; SSF53822; 1.
DR PROSITE; PS00980; G_PROTEIN_RECEP_F3_2; 1.
DR PROSITE; PS50259; G_PROTEIN_RECEP_F3_4; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Signal; Transducer; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..928
FT /note="G-protein coupled receptor family C group 6 member
FT A"
FT /id="PRO_0000043198"
FT TOPO_DOM 21..594
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 595..615
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 616..630
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 631..651
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 652..669
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 670..690
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 691..706
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 707..727
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 728..750
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 751..771
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 772..784
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 785..805
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 806..812
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 813..833
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 834..928
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 332
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 555
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 131
FT /note="Interchain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
SQ SEQUENCE 928 AA; 104353 MW; 0B13BF36E6A60BC4 CRC64;
MALSFVFITC FMILLDTSQS CHTPDDFVAI TSPGHIMIGG LFAIHEKMLS SDDHPRQPQI
QKCVGFEISV FLQTLAMIHS IEMINNSSLL SGVKLGYEIY DTCTEVTAAM AATLRFLSKF
NCSRETVIFQ CDYSSYVPRV KAIIGAGYSE ISMAVSRMLN LQLMPQVSYE STAEILSDKI
RFPSFLRTVP SDFYQTKAMA HLIRQSGWNW VGAITTDDDY GRLALNTFAI QAAENNVCIA
FKEVLPAFLS DNTIEVRINQ TLEKIIAEAQ VNVIVVFLRK FHVFNLFNKA IERKISKIWI
ASDNWSTAAK IITIPNVKKL GKVVGFTFRR GNMSSFHSFL QTLHMYPSDN NKPLHEFAML
FSACKHIKDG DLSQCISNYS QATWTYDTTK TIETHLFKRN DFLWHYTEPG LIHSIQLAVL
ALGHAIRDLC QDRDCQKPNA FQPWELLAVL KNVTFTDGKN SFHFDAHGDL NTGYEVVLWK
ETNGLMTVTK MAEYDLQHDV FITTNQETKH EFRKLKQILS KCSKECIPGQ MKKATGSQHS
CCYECVNCPE NHYSNETDMD HCLVCNNETH WAPVRSTMCF EKEVEYLDWD DSLALLLIAL
SLLGIAFVLA VGIIFTRNLK TPVVKSSGGL VVCYVMLACH ALNFASTGFF IGEPQDFTCK
TRQTLFGVSF TLCVSCILTK SLKILLAFSF DPTLKTFLKC LYRPVPIVLT CTGIQVVICT
LWLVLAAPTV EENTSLPRVI ILECEEGSAL AFGTMLGYIA VLAFICFVFA FKGRKLPENY
NEAKFLTFGM LIYFIAWITF IPVYATTFGK YLPAVEIIVI LISNYGILCC TFFPKCYIIL
CKQKTNTKSV FLQMVYNYSA HSVDSLALSH VSLDSASHST ATTNPRPGNK TAACQNYKHL
PVQVLAHTGM EKTMHASKTL HQKRSSSI
