GPDA1_ARATH
ID GPDA1_ARATH Reviewed; 400 AA.
AC Q9SCX9; Q8RXI6;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplastic;
DE EC=1.1.1.8;
DE Flags: Precursor;
GN Name=DHAPRD; OrderedLocusNames=At5g40610; ORFNames=MNF13.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RA Wei Y., Periappuram C., Datla R., Selvaraj G., Zou J.;
RT "Molecular and biochemical characterizations of a plastidic glycerol-3-
RT phosphate dehydrogenase from Arabidopsis.";
RL Plant Physiol. Biochem. 39:841-848(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Involved in glycerolipid metabolism. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NAD(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate
CC + H(+) + NADH; Xref=Rhea:RHEA:11092, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57597, ChEBI:CHEBI:57642,
CC ChEBI:CHEBI:57945; EC=1.1.1.8;
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=12.8 uM for glycerone phosphate {ECO:0000269|Ref.1};
CC Vmax=3.2 umol/sec/mg enzyme toward glycerone phosphate
CC {ECO:0000269|Ref.1};
CC -!- PATHWAY: Membrane lipid metabolism; glycerophospholipid metabolism.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000269|Ref.1}.
CC -!- TISSUE SPECIFICITY: Expressed in young seedlings, flowers and siliques.
CC Expressed at low levels in roots. {ECO:0000269|Ref.1}.
CC -!- SIMILARITY: Belongs to the NAD-dependent glycerol-3-phosphate
CC dehydrogenase family. {ECO:0000305}.
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DR EMBL; AJ242602; CAB64726.1; -; mRNA.
DR EMBL; AB009052; BAB08532.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94573.1; -; Genomic_DNA.
DR EMBL; BT000967; AAN41367.1; -; mRNA.
DR EMBL; AY080863; AAL87336.1; -; mRNA.
DR RefSeq; NP_198877.1; NM_123425.5.
DR AlphaFoldDB; Q9SCX9; -.
DR SMR; Q9SCX9; -.
DR STRING; 3702.AT5G40610.1; -.
DR PaxDb; Q9SCX9; -.
DR PRIDE; Q9SCX9; -.
DR ProteomicsDB; 248463; -.
DR EnsemblPlants; AT5G40610.1; AT5G40610.1; AT5G40610.
DR GeneID; 834060; -.
DR Gramene; AT5G40610.1; AT5G40610.1; AT5G40610.
DR KEGG; ath:AT5G40610; -.
DR Araport; AT5G40610; -.
DR TAIR; locus:2168758; AT5G40610.
DR eggNOG; KOG2711; Eukaryota.
DR HOGENOM; CLU_033449_2_2_1; -.
DR InParanoid; Q9SCX9; -.
DR OMA; FIHKVCD; -.
DR OrthoDB; 476066at2759; -.
DR PhylomeDB; Q9SCX9; -.
DR UniPathway; UPA00940; -.
DR PRO; PR:Q9SCX9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9SCX9; baseline and differential.
DR Genevisible; Q9SCX9; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR GO; GO:0004367; F:glycerol-3-phosphate dehydrogenase [NAD+] activity; IBA:GO_Central.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0042803; F:protein homodimerization activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro.
DR GO; GO:0006072; P:glycerol-3-phosphate metabolic process; IBA:GO_Central.
DR GO; GO:0006650; P:glycerophospholipid metabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006116; P:NADH oxidation; IBA:GO_Central.
DR GO; GO:0008654; P:phospholipid biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1040.10; -; 1.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR013328; 6PGD_dom2.
DR InterPro; IPR006168; G3P_DH_NAD-dep.
DR InterPro; IPR006109; G3P_DH_NAD-dep_C.
DR InterPro; IPR017751; G3P_DH_NAD-dep_euk.
DR InterPro; IPR011128; G3P_DH_NAD-dep_N.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF07479; NAD_Gly3P_dh_C; 1.
DR Pfam; PF01210; NAD_Gly3P_dh_N; 1.
DR PIRSF; PIRSF000114; Glycerol-3-P_dh; 1.
DR PRINTS; PR00077; GPDHDRGNASE.
DR SUPFAM; SSF48179; SSF48179; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR TIGRFAMs; TIGR03376; glycerol3P_DH; 1.
DR PROSITE; PS00957; NAD_G3PDH; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Lipid biosynthesis; Lipid metabolism; NAD; Oxidoreductase;
KW Phospholipid biosynthesis; Phospholipid metabolism; Plastid;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..32
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 33..400
FT /note="Glycerol-3-phosphate dehydrogenase [NAD(+)] 1,
FT chloroplastic"
FT /id="PRO_0000287871"
FT ACT_SITE 257
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 61..66
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 92
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 149
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 172
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 172
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 205
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 321..322
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 321
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 350
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 352
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
SQ SEQUENCE 400 AA; 43791 MW; 9A37BC876E8663CC CRC64;
MRFRSFFFSS SIFSLSHSRS PSLSSSRFSS LSAAMSPALE KSRQGNGGCN DDSKSKVTVV
GSGNWGSVAA KLIASNALKL PSFHDEVRMW VFEEVLPNGE KLNDVINKTN ENVKYLPGIK
LGRNVVADPD LENAVKDANM LVFVTPHQFM DGICKKLDGK ITGDVEAISL VKGMEVKKEG
PCMISSLISK QLGINCCVLM GANIANEIAV EKFSEATVGY RGSREIADTW VQLFSTPYFM
VTPVHDVEGV ELCGTLKNVV AIAAGFVDGL EMGNNTKAAI MRIGLREMKA LSKLLFPSVK
DSTFFESCGV ADVITTCLGG RNRRVAEAFA KSRGKRSFDE LEAEMLQGQK LQGVSTAREV
YEVLKHCGWL EMFPLFSTVH QICTGRLQPE AIVQYRENKL
