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GPDA_DROME
ID   GPDA_DROME              Reviewed;         363 AA.
AC   P13706; Q27590; Q27925; Q95077; Q95TV4; Q9VML0;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 3.
DT   03-AUG-2022, entry version 214.
DE   RecName: Full=Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic;
DE            Short=GPD-C;
DE            Short=GPDH-C;
DE            EC=1.1.1.8;
GN   Name=Gpdh1 {ECO:0000312|FlyBase:FBgn0001128};
GN   Synonyms=Gpdh {ECO:0000303|PubMed:2500660};
GN   ORFNames=CG9042 {ECO:0000312|FlyBase:FBgn0001128};
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, AND DEVELOPMENTAL
RP   STAGE.
RC   STRAIN=Canton-S;
RX   PubMed=2500660; DOI=10.1073/pnas.86.13.5020;
RA   von Kalm L., Weaver J., Demarco J., Macintyre R.J., Sullivan D.T.;
RT   "Structural characterization of the alpha-glycerol-3-phosphate
RT   dehydrogenase-encoding gene of Drosophila melanogaster.";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:5020-5024(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 21-133; 139-141 AND
RP   148-350, ALTERNATIVE SPLICING, AND DEFINITION OF ALLELES FAST AND SLOW.
RC   STRAIN=Canton-S, Ogasawara, and Oregon-R;
RX   PubMed=2511555; DOI=10.1093/nar/17.21.8553;
RA   Bewley G.C., Cook J.L., Kusakabe S., Mukai T., Rigby D.L., Chambers G.K.;
RT   "Sequence, structure and evolution of the gene coding for sn-glycerol-3-
RT   phosphate dehydrogenase in Drosophila melanogaster.";
RL   Nucleic Acids Res. 17:8553-8567(1989).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTANTS.
RC   STRAIN=Tasmania;
RX   PubMed=8094564; DOI=10.1098/rspb.1993.0006;
RA   Reed D.S., Gibson J.B.;
RT   "Defective P element insertions affect the expression of sn-glycerol-3-
RT   phosphate dehydrogenase alleles in natural populations of Drosophila
RT   melanogaster.";
RL   Proc. R. Soc. B 251:39-45(1993).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE GPDH-ACB62).
RC   STRAIN=Cardwell;
RX   PubMed=7633467; DOI=10.1016/0965-1748(95)00002-d;
RA   Symonds J.E., Gibson J.B., Wilks A.V., Wilanowski T.M.;
RT   "Molecular analysis of a Drosophila melanogaster sn-glycerol-3-phosphate
RT   dehydrogenase allozyme variant that has cold labile activity.";
RL   Insect Biochem. Mol. Biol. 25:789-798(1995).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [6]
RP   GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM GPDH-2).
RC   STRAIN=Berkeley; TISSUE=Ovary;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [8]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2-363 (ALLELES GPDH-MB5 AND
RP   GPDH-ACYG22).
RC   STRAIN=Cygnet, and Mission beach;
RX   PubMed=7993372; DOI=10.1007/bf00554620;
RA   Reed D.S., Gibson J.B.;
RT   "Molecular heterogeneity of naturally occurring sn-glycerol-3-phosphate
RT   dehydrogenase low-activity variants in Drosophila melanogaster.";
RL   Biochem. Genet. 32:161-179(1994).
RN   [9]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 206-245.
RC   STRAIN=Canton-S;
RX   PubMed=3755720; DOI=10.1016/s0021-9258(18)67307-7;
RA   Cook J.L., Shaffer J.B., Bewley G.C., MacIntyre R.J., Wright D.A.;
RT   "Isolation of a genomic clone for Drosophila sn-glycerol-3-phosphate
RT   dehydrogenase using synthetic oligonucleotides.";
RL   J. Biol. Chem. 261:11751-11755(1986).
RN   [10]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 298-350, ALTERNATIVE SPLICING, AND
RP   DEVELOPMENTAL STAGE.
RC   STRAIN=Canton-S;
RX   PubMed=2839508; DOI=10.1016/s0021-9258(18)38049-9;
RA   Cook J.L., Bewley G.C., Shaffer J.B.;
RT   "Drosophila sn-glycerol-3-phosphate dehydrogenase isozymes are generated by
RT   alternate pathways of RNA processing resulting in different carboxyl-
RT   terminal amino acid sequences.";
RL   J. Biol. Chem. 263:10858-10864(1988).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate
CC         + H(+) + NADH; Xref=Rhea:RHEA:11092, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57597, ChEBI:CHEBI:57642,
CC         ChEBI:CHEBI:57945; EC=1.1.1.8;
CC   -!- PATHWAY: Phospholipid metabolism; alpha-glycerophosphate cycle.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=4;
CC       Name=GPDH-4;
CC         IsoId=P13706-1; Sequence=Displayed;
CC       Name=GPDH-1; Synonyms=GPDH-411, C;
CC         IsoId=P13706-2; Sequence=VSP_001589;
CC       Name=GPDH-2; Synonyms=GPDH-37, B;
CC         IsoId=P13706-3; Sequence=VSP_001591;
CC       Name=GPDH-3; Synonyms=GPDH-1A, A;
CC         IsoId=P13706-4; Sequence=VSP_001590;
CC   -!- TISSUE SPECIFICITY: Isoform GPDH-1 is predominant in thorax and isoform
CC       GPDH-3 in abdomen.
CC   -!- DEVELOPMENTAL STAGE: Isoform GPDH-2 and isoform GPDH-3 are expressed in
CC       both larvae and adults. Isoform GPDH-1 is expressed only in adults.
CC       {ECO:0000269|PubMed:2500660, ECO:0000269|PubMed:2839508}.
CC   -!- POLYMORPHISM: There are two common alleles; fast and slow. The sequence
CC       of fast is shown here.
CC   -!- SIMILARITY: Belongs to the NAD-dependent glycerol-3-phosphate
CC       dehydrogenase family. {ECO:0000305}.
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DR   EMBL; J04567; AAA28591.1; -; Genomic_DNA.
DR   EMBL; J04567; AAA28592.1; -; Genomic_DNA.
DR   EMBL; J04567; AAA28593.1; -; Genomic_DNA.
DR   EMBL; X14179; CAA32379.1; -; Genomic_DNA.
DR   EMBL; X14179; CAA32380.1; -; Genomic_DNA.
DR   EMBL; X14179; CAA32381.1; -; Genomic_DNA.
DR   EMBL; X67650; CAA47892.1; -; Genomic_DNA.
DR   EMBL; X61223; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; X61224; CAA43536.1; -; Genomic_DNA.
DR   EMBL; AE014134; AAF52304.1; -; Genomic_DNA.
DR   EMBL; AE014134; AAN10562.1; -; Genomic_DNA.
DR   EMBL; AE014134; AAN10563.1; -; Genomic_DNA.
DR   EMBL; AY058492; AAL13721.1; -; mRNA.
DR   EMBL; X80204; CAA56497.1; -; Genomic_DNA.
DR   EMBL; M13786; AAA28590.1; -; Genomic_DNA.
DR   EMBL; J03927; AAA28553.1; -; Genomic_DNA.
DR   EMBL; J03927; AAA28554.1; -; Genomic_DNA.
DR   EMBL; J03927; AAA28555.1; -; Genomic_DNA.
DR   PIR; S06758; S06758.
DR   PIR; S06759; S06759.
DR   PIR; S06760; S06760.
DR   PIR; S21963; S21963.
DR   RefSeq; NP_001260111.1; NM_001273182.1. [P13706-4]
DR   RefSeq; NP_001260112.1; NM_001273183.2. [P13706-3]
DR   RefSeq; NP_001260113.1; NM_001273184.2. [P13706-2]
DR   RefSeq; NP_476565.1; NM_057217.4. [P13706-3]
DR   RefSeq; NP_476566.1; NM_057218.4. [P13706-2]
DR   RefSeq; NP_476567.1; NM_057219.4. [P13706-4]
DR   AlphaFoldDB; P13706; -.
DR   SMR; P13706; -.
DR   BioGRID; 59989; 73.
DR   DIP; DIP-21689N; -.
DR   IntAct; P13706; 1.
DR   STRING; 7227.FBpp0305431; -.
DR   PaxDb; P13706; -.
DR   DNASU; 33824; -.
DR   EnsemblMetazoa; FBtr0079146; FBpp0078777; FBgn0001128. [P13706-3]
DR   EnsemblMetazoa; FBtr0079147; FBpp0078778; FBgn0001128. [P13706-2]
DR   EnsemblMetazoa; FBtr0079148; FBpp0078779; FBgn0001128. [P13706-4]
DR   EnsemblMetazoa; FBtr0333228; FBpp0305430; FBgn0001128. [P13706-4]
DR   EnsemblMetazoa; FBtr0333229; FBpp0305431; FBgn0001128. [P13706-3]
DR   EnsemblMetazoa; FBtr0333230; FBpp0305432; FBgn0001128. [P13706-2]
DR   GeneID; 33824; -.
DR   KEGG; dme:Dmel_CG9042; -.
DR   CTD; 33824; -.
DR   FlyBase; FBgn0001128; Gpdh1.
DR   VEuPathDB; VectorBase:FBgn0001128; -.
DR   eggNOG; KOG2711; Eukaryota.
DR   GeneTree; ENSGT00390000003114; -.
DR   InParanoid; P13706; -.
DR   OMA; NRMFGNM; -.
DR   PhylomeDB; P13706; -.
DR   BRENDA; 1.1.1.8; 1994.
DR   Reactome; R-DME-1483166; Synthesis of PA.
DR   SignaLink; P13706; -.
DR   UniPathway; UPA00086; -.
DR   BioGRID-ORCS; 33824; 1 hit in 3 CRISPR screens.
DR   ChiTaRS; Gpdh; fly.
DR   GenomeRNAi; 33824; -.
DR   PRO; PR:P13706; -.
DR   Proteomes; UP000000803; Chromosome 2L.
DR   Bgee; FBgn0001128; Expressed in capitellum (Drosophila) and 24 other tissues.
DR   ExpressionAtlas; P13706; baseline and differential.
DR   Genevisible; P13706; DM.
DR   GO; GO:0005829; C:cytosol; IDA:FlyBase.
DR   GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR   GO; GO:0031430; C:M band; IDA:FlyBase.
DR   GO; GO:0030018; C:Z disc; IDA:FlyBase.
DR   GO; GO:0004367; F:glycerol-3-phosphate dehydrogenase [NAD+] activity; IDA:FlyBase.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0042803; F:protein homodimerization activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006067; P:ethanol metabolic process; IMP:CACAO.
DR   GO; GO:0007629; P:flight behavior; IMP:FlyBase.
DR   GO; GO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro.
DR   GO; GO:0006072; P:glycerol-3-phosphate metabolic process; IBA:GO_Central.
DR   GO; GO:0006650; P:glycerophospholipid metabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006734; P:NADH metabolic process; IDA:FlyBase.
DR   GO; GO:0006116; P:NADH oxidation; IBA:GO_Central.
DR   GO; GO:0055093; P:response to hyperoxia; IMP:FlyBase.
DR   GO; GO:0006641; P:triglyceride metabolic process; IMP:FlyBase.
DR   Gene3D; 1.10.1040.10; -; 1.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR013328; 6PGD_dom2.
DR   InterPro; IPR006168; G3P_DH_NAD-dep.
DR   InterPro; IPR006109; G3P_DH_NAD-dep_C.
DR   InterPro; IPR017751; G3P_DH_NAD-dep_euk.
DR   InterPro; IPR011128; G3P_DH_NAD-dep_N.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF07479; NAD_Gly3P_dh_C; 1.
DR   Pfam; PF01210; NAD_Gly3P_dh_N; 1.
DR   PIRSF; PIRSF000114; Glycerol-3-P_dh; 1.
DR   PRINTS; PR00077; GPDHDRGNASE.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR03376; glycerol3P_DH; 1.
DR   PROSITE; PS00957; NAD_G3PDH; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cytoplasm; Direct protein sequencing; NAD;
KW   Oxidoreductase; Reference proteome.
FT   CHAIN           1..363
FT                   /note="Glycerol-3-phosphate dehydrogenase [NAD(+)],
FT                   cytoplasmic"
FT                   /id="PRO_0000138075"
FT   ACT_SITE        206
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         11..16
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         98
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         121
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         121
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         155
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         270..271
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         270
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         299
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         351..363
FT                   /note="Missing (in isoform GPDH-3)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_001590"
FT   VAR_SEQ         351..360
FT                   /note="Missing (in isoform GPDH-1)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_001589"
FT   VAR_SEQ         361..363
FT                   /note="Missing (in isoform GPDH-2)"
FT                   /evidence="ECO:0000303|PubMed:12537569"
FT                   /id="VSP_001591"
FT   VARIANT         14
FT                   /note="N -> Y (in allele GPDH-ACYG22)"
FT   VARIANT         266
FT                   /note="C -> G (in allele GPDH-ACB62)"
FT   VARIANT         273
FT                   /note="R -> C (in allele GPDH-ACYG22)"
FT   VARIANT         337
FT                   /note="N -> K (in allele GPDH-AT198, allele GPDH-ACB62 and
FT                   allele GPDH-S)"
FT   CONFLICT        351..363
FT                   /note="DTSIMPSPKLQNL -> GYVHHAVAKTCKICKSPKEAETFLTFLCTTENKCI
FT                   FSAQRQLDESLDSIF (in Ref. 3; CAA47892)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   363 AA;  39684 MW;  49FA09CE7FB6FDF4 CRC64;
     MADKVNVCIV GSGNWGSAIA KIVGANAAAL PEFEERVTMF VYEELIDGKK LTEIINETHE
     NVKYLKGHKL PPNVVAVPDL VEAAKNADIL IFVVPHQFIP NFCKQLLGKI KPNAIAISLI
     KGFDKAEGGG IDLISHIITR HLKIPCAVLM GANLANEVAE GNFCETTIGC TDKKYGKVLR
     DLFQANHFRV VVVDDADAVE VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF
     VDVFYPGSKL STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEELEK EMLNGQKLQG
     PPTAEEVNYM LKNKGLEDKF PLFTAIHKIC TNQLKPNDLI DCIRNHPEHM DTSIMPSPKL
     QNL
 
 
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