3S1CC_NAJAT
ID 3S1CC_NAJAT Reviewed; 82 AA.
AC P80958; O42285; Q6S468; Q9YHU9; Q9YHV0;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=Cobrotoxin-b;
DE Short=CBT-b;
DE AltName: Full=Atratoxin-b;
DE AltName: Full=Cobrotoxin III;
DE Short=CBT-III;
DE AltName: Full=Cobrotoxin IV;
DE Short=CBT IV;
DE AltName: Full=NT3;
DE AltName: Full=Short neurotoxin;
DE Flags: Precursor;
OS Naja atra (Chinese cobra).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX NCBI_TaxID=8656;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 22-82, FUNCTION, AND
RP SUBCELLULAR LOCATION.
RC TISSUE=Liver, and Venom;
RX PubMed=9498573; DOI=10.1093/oxfordjournals.jbchem.a021889;
RA Chang L.-S., Chou Y.-C., Lin S.-R., Wu B.-N., Lin J., Hong E., Sun Y.-J.,
RA Hsiao C.-D.;
RT "A novel neurotoxin, cobrotoxin b, from Naja naja atra (Taiwan cobra)
RT venom: purification, characterization, and gene organization.";
RL J. Biochem. 122:1252-1259(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND SEQUENCE REVISION TO 67.
RC TISSUE=Venom gland;
RA Chu R.C., Yang C.-C.;
RL Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 22-82.
RC TISSUE=Venom gland;
RA Pan H., Wu J., Yang G.Z., Wu X.F.;
RT "Molecular cloning, sequence analysis and expression of cobrotoxin from
RT Naja naja atra.";
RL Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 10-82, X-RAY CRYSTALLOGRAPHY (0.92 ANGSTROMS)
RP OF 22-82, AND DISULFIDE BOND.
RC TISSUE=Venom gland;
RX PubMed=15252034; DOI=10.1074/jbc.m403863200;
RA Lou X., Liu Q., Tu X., Wang J., Teng M., Niu L., Schuller D.J., Huang Q.,
RA Hao Q.;
RT "The atomic resolution crystal structure of atratoxin determined by single
RT wavelength anomalous diffraction phasing.";
RL J. Biol. Chem. 279:39094-39104(2004).
RN [5]
RP PROTEIN SEQUENCE OF 22-35, STRUCTURE BY NMR OF 22-82, TOXIC DOSE, MASS
RP SPECTROMETRY, AND DISULFIDE BOND.
RC TISSUE=Venom;
RX PubMed=12777767; DOI=10.1107/s0907444903005687;
RA Lou X., Tu X., Pan G., Xu C., Fan R., Lu W., Deng W., Rao P., Teng M.,
RA Niu L.;
RT "Purification, N-terminal sequencing, crystallization and preliminary
RT structural determination of atratoxin-b, a short-chain alpha-neurotoxin
RT from Naja atra venom.";
RL Acta Crystallogr. D 59:1038-1042(2003).
CC -!- FUNCTION: Binds to muscle nicotinic acetylcholine receptor (nAChR) and
CC inhibit acetylcholine from binding to the receptor, thereby impairing
CC neuromuscular transmission. Produces peripheral paralysis by blocking
CC neuromuscular transmission at the postsynaptic site. Has a lower
CC toxicity than cobrotoxin. {ECO:0000269|PubMed:9498573}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9498573}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=6950; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:12777767};
CC -!- TOXIC DOSE: LD(50) is 0.18 mg/kg by intravenous injection into mice.
CC {ECO:0000269|PubMed:12777767}.
CC -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC subfamily. Type I alpha-neurotoxin sub-subfamily. {ECO:0000305}.
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DR EMBL; Y13399; CAA73829.2; -; Genomic_DNA.
DR EMBL; AF031472; AAB86636.1; -; mRNA.
DR EMBL; AF088997; AAD09179.1; -; mRNA.
DR EMBL; AF088998; AAD09180.1; -; mRNA.
DR EMBL; AY471579; AAR33036.1; -; mRNA.
DR PIR; JC5892; JC5892.
DR PDB; 1ONJ; X-ray; 1.55 A; A=22-82.
DR PDB; 1VB0; X-ray; 0.92 A; A=22-82.
DR PDBsum; 1ONJ; -.
DR PDBsum; 1VB0; -.
DR AlphaFoldDB; P80958; -.
DR SMR; P80958; -.
DR EvolutionaryTrace; P80958; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR CDD; cd00206; snake_toxin; 1.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR003571; Snake_3FTx.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR InterPro; IPR018354; Snake_toxin_con_site.
DR SUPFAM; SSF57302; SSF57302; 1.
DR PROSITE; PS00272; SNAKE_TOXIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylcholine receptor inhibiting toxin;
KW Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW Neurotoxin; Postsynaptic neurotoxin; Secreted; Signal; Toxin.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:12777767,
FT ECO:0000269|PubMed:9498573"
FT CHAIN 22..82
FT /note="Cobrotoxin-b"
FT /evidence="ECO:0000269|PubMed:9498573"
FT /id="PRO_0000035456"
FT DISULFID 24..44
FT /evidence="ECO:0000269|PubMed:12777767,
FT ECO:0000269|PubMed:15252034, ECO:0000312|PDB:1ONJ,
FT ECO:0000312|PDB:1VB0"
FT DISULFID 38..61
FT /evidence="ECO:0000269|PubMed:12777767,
FT ECO:0000269|PubMed:15252034, ECO:0000312|PDB:1ONJ,
FT ECO:0000312|PDB:1VB0"
FT DISULFID 63..74
FT /evidence="ECO:0000269|PubMed:12777767,
FT ECO:0000269|PubMed:15252034, ECO:0000312|PDB:1ONJ,
FT ECO:0000312|PDB:1VB0"
FT DISULFID 75..80
FT /evidence="ECO:0000269|PubMed:12777767,
FT ECO:0000269|PubMed:15252034, ECO:0000312|PDB:1ONJ,
FT ECO:0000312|PDB:1VB0"
FT CONFLICT 22
FT /note="L -> M (in Ref. 3; AAD09179/AAD09180)"
FT /evidence="ECO:0000305"
FT CONFLICT 32
FT /note="T -> A (in Ref. 3; AAD09179)"
FT /evidence="ECO:0000305"
FT CONFLICT 34
FT /note="T -> TI (in Ref. 5; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 36..39
FT /note="KTCS -> TGCSG (in Ref. 3; AAD09180)"
FT /evidence="ECO:0000305"
FT STRAND 23..25
FT /evidence="ECO:0007829|PDB:1VB0"
FT STRAND 35..37
FT /evidence="ECO:0007829|PDB:1VB0"
FT STRAND 44..51
FT /evidence="ECO:0007829|PDB:1VB0"
FT STRAND 54..62
FT /evidence="ECO:0007829|PDB:1VB0"
FT STRAND 71..75
FT /evidence="ECO:0007829|PDB:1VB0"
FT TURN 78..81
FT /evidence="ECO:0007829|PDB:1VB0"
SQ SEQUENCE 82 AA; 9139 MW; 1FFA21189C08B6E8 CRC64;
MKTLLLTLLV VTIVCLDLGY TLECHNQQSS QTPTTKTCSG ETNCYKKWWS DHRGTIIERG
CGCPKVKPGV NLNCCTTDRC NN
