ID   GPHA2_MOUSE             Reviewed;         128 AA.
AC   Q925Q5;
DT   10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 133.
DE   RecName: Full=Glycoprotein hormone alpha-2;
DE   AltName: Full=Putative secreted protein Zsig51;
DE   AltName: Full=Thyrostimulin subunit alpha;
DE   Flags: Precursor;
GN   Name=Gpha2; Synonyms=Gpa2, Zsig51;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Ching A., Gilbert T., Sheppard P., Webster P., O'Hara P.J.;
RT   "A novel cysteine knot protein expressed in pancreatic acinar cells.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Thymus;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Functions as a heterodimeric glycoprotein hormone with GPHB5
CC       able to bind and activate the thyroid-stimulating hormone receptor
CC       (TSHR), leading to increased cAMP production. Plays a central role in
CC       controlling thyroid cell metabolism. {ECO:0000250|UniProtKB:Q96T91}.
CC   -!- SUBUNIT: Heterodimer with GPHB5; this heterodimer interacts with
CC       thyroid-stimulating hormone receptor (TSHR), and hence stimulates cAMP
CC       production. {ECO:0000250|UniProtKB:Q96T91}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycoprotein hormones subunit alpha family.
CC       {ECO:0000305}.
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DR   EMBL; AF260740; AAK51639.1; -; mRNA.
DR   EMBL; BC062804; AAH62804.1; -; mRNA.
DR   CCDS; CCDS29498.1; -.
DR   RefSeq; NP_569720.1; NM_130453.3.
DR   AlphaFoldDB; Q925Q5; -.
DR   STRING; 10090.ENSMUSP00000025698; -.
DR   GlyGen; Q925Q5; 2 sites.
DR   PaxDb; Q925Q5; -.
DR   PRIDE; Q925Q5; -.
DR   Antibodypedia; 65614; 40 antibodies from 9 providers.
DR   Ensembl; ENSMUST00000025698; ENSMUSP00000025698; ENSMUSG00000024784.
DR   Ensembl; ENSMUST00000113526; ENSMUSP00000109154; ENSMUSG00000024784.
DR   GeneID; 170458; -.
DR   KEGG; mmu:170458; -.
DR   UCSC; uc008ghs.1; mouse.
DR   CTD; 170589; -.
DR   MGI; MGI:2156541; Gpha2.
DR   VEuPathDB; HostDB:ENSMUSG00000024784; -.
DR   eggNOG; ENOG502S2RQ; Eukaryota.
DR   GeneTree; ENSGT00390000009379; -.
DR   HOGENOM; CLU_134026_1_0_1; -.
DR   InParanoid; Q925Q5; -.
DR   OMA; ISQCCTI; -.
DR   OrthoDB; 1435006at2759; -.
DR   PhylomeDB; Q925Q5; -.
DR   TreeFam; TF332313; -.
DR   Reactome; R-MMU-375281; Hormone ligand-binding receptors.
DR   Reactome; R-MMU-418555; G alpha (s) signalling events.
DR   BioGRID-ORCS; 170458; 1 hit in 72 CRISPR screens.
DR   PRO; PR:Q925Q5; -.
DR   Proteomes; UP000000589; Chromosome 19.
DR   RNAct; Q925Q5; protein.
DR   Bgee; ENSMUSG00000024784; Expressed in adrenal gland and 39 other tissues.
DR   ExpressionAtlas; Q925Q5; baseline and differential.
DR   Genevisible; Q925Q5; MM.
DR   GO; GO:0005576; C:extracellular region; ISO:MGI.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0051427; F:hormone receptor binding; IBA:GO_Central.
DR   GO; GO:0046982; F:protein heterodimerization activity; ISO:MGI.
DR   GO; GO:0031531; F:thyrotropin-releasing hormone receptor binding; ISO:MGI.
DR   GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; ISO:MGI.
DR   GO; GO:0007166; P:cell surface receptor signaling pathway; ISO:MGI.
DR   Gene3D; 2.10.90.10; -; 1.
DR   InterPro; IPR006207; Cys_knot_C.
DR   InterPro; IPR029034; Cystine-knot_cytokine.
DR   InterPro; IPR000476; Glyco_hormone.
DR   SUPFAM; SSF57501; SSF57501; 1.
DR   PROSITE; PS01185; CTCK_1; 1.
DR   PROSITE; PS01225; CTCK_2; 1.
DR   PROSITE; PS50277; GLYCO_HORMONE_ALPHA_3; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Hormone; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..128
FT                   /note="Glycoprotein hormone alpha-2"
FT                   /id="PRO_0000011673"
FT   CARBOHYD        36
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        80
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        30..88
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   DISULFID        47..102
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   DISULFID        56..118
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   DISULFID        60..120
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
SQ   SEQUENCE   128 AA;  14030 MW;  B4008BA348D3EF82 CRC64;
     MPMAPRVLLL CLLGLAVTEG HSPETAIPGC HLHPFNVTVR SDRLGTCQGS HVAQACVGHC
     ESSAFPSRYS VLVASGYRHN ITSSSQCCTI SSLRKVRVWL QCVGNQRGEL EIFTARACQC
     DMCRFSRY