ID   GPHA2_RAT               Reviewed;         130 AA.
AC   Q925Q4;
DT   10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Glycoprotein hormone alpha-2;
DE   AltName: Full=Putative secreted protein Zsig51;
DE   AltName: Full=Thyrostimulin subunit alpha;
DE   Flags: Precursor;
GN   Name=Gpha2; Synonyms=Gpa2, Zsig51;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Dasovich M., Ching A., Lok S., Sheppard P., Webster P.;
RT   "A novel cysteine knot protein expressed in pancreatic acinar cells.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Functions as a heterodimeric glycoprotein hormone with GPHB5
CC       able to bind and activate the thyroid-stimulating hormone receptor
CC       (TSHR), leading to increased cAMP production. Plays a central role in
CC       controlling thyroid cell metabolism. {ECO:0000250|UniProtKB:Q96T91}.
CC   -!- SUBUNIT: Heterodimer with GPHB5; this heterodimer interacts with
CC       thyroid-stimulating hormone receptor (TSHR), and hence stimulates cAMP
CC       production. {ECO:0000250|UniProtKB:Q96T91}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycoprotein hormones subunit alpha family.
CC       {ECO:0000305}.
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DR   EMBL; AF260741; AAK51640.1; -; mRNA.
DR   RefSeq; NP_598303.1; NM_133619.2.
DR   RefSeq; XP_008758291.1; XM_008760069.2.
DR   RefSeq; XP_008758292.1; XM_008760070.2.
DR   AlphaFoldDB; Q925Q4; -.
DR   STRING; 10116.ENSRNOP00000028536; -.
DR   GlyGen; Q925Q4; 2 sites.
DR   PaxDb; Q925Q4; -.
DR   Ensembl; ENSRNOT00000028536; ENSRNOP00000028536; ENSRNOG00000021020.
DR   GeneID; 171158; -.
DR   KEGG; rno:171158; -.
DR   UCSC; RGD:621748; rat.
DR   CTD; 170589; -.
DR   RGD; 621748; Gpha2.
DR   eggNOG; ENOG502S2RQ; Eukaryota.
DR   GeneTree; ENSGT00390000009379; -.
DR   HOGENOM; CLU_134026_1_0_1; -.
DR   InParanoid; Q925Q4; -.
DR   OMA; ISQCCTI; -.
DR   OrthoDB; 1435006at2759; -.
DR   PhylomeDB; Q925Q4; -.
DR   TreeFam; TF332313; -.
DR   Reactome; R-RNO-375281; Hormone ligand-binding receptors.
DR   PRO; PR:Q925Q4; -.
DR   Proteomes; UP000002494; Chromosome 1.
DR   Bgee; ENSRNOG00000021020; Expressed in thymus and 7 other tissues.
DR   ExpressionAtlas; Q925Q4; baseline and differential.
DR   Genevisible; Q925Q4; RN.
DR   GO; GO:0005576; C:extracellular region; ISO:RGD.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0051427; F:hormone receptor binding; IBA:GO_Central.
DR   GO; GO:0046982; F:protein heterodimerization activity; ISO:RGD.
DR   GO; GO:0031531; F:thyrotropin-releasing hormone receptor binding; ISO:RGD.
DR   GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; ISO:RGD.
DR   GO; GO:0007166; P:cell surface receptor signaling pathway; ISO:RGD.
DR   Gene3D; 2.10.90.10; -; 1.
DR   InterPro; IPR006207; Cys_knot_C.
DR   InterPro; IPR029034; Cystine-knot_cytokine.
DR   InterPro; IPR000476; Glyco_hormone.
DR   SUPFAM; SSF57501; SSF57501; 1.
DR   PROSITE; PS01185; CTCK_1; 1.
DR   PROSITE; PS01225; CTCK_2; 1.
DR   PROSITE; PS50277; GLYCO_HORMONE_ALPHA_3; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Hormone; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..130
FT                   /note="Glycoprotein hormone alpha-2"
FT                   /id="PRO_0000011674"
FT   CARBOHYD        38
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        82
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        32..90
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   DISULFID        49..104
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   DISULFID        58..120
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   DISULFID        62..122
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
SQ   SEQUENCE   130 AA;  14199 MW;  0BC46977131ED3DE CRC64;
     MPMAPRVLLF CLLGLAVTEG HGLEAAVPIP GCHLHPFNVT VRSDRHGTCQ GSHVAQACVG
     HCESSAFPSR YSVLVASGYR HNITSVSQCC TISSLKKVRV WLHCVGNQRG ELEIFTARAC
     QCDMCRLSRY