GPHR_MOUSE
ID GPHR_MOUSE Reviewed; 455 AA.
AC Q8BS95; Q9D455;
DT 07-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 07-FEB-2006, sequence version 2.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Golgi pH regulator;
DE AltName: Full=Protein GPR89;
GN Name=Gpr89 {ECO:0000312|MGI:MGI:1914799};
GN Synonyms=Gphr, Gpr89a {ECO:0000312|MGI:MGI:1914799};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH RABL3, AND
RP MUTAGENESIS OF 273-GLU--MET-303.
RX PubMed=32220963; DOI=10.1073/pnas.2000703117;
RA Zhong X., Su L., Yang Y., Nair-Gill E., Tang M., Anderton P., Li X.,
RA Wang J., Zhan X., Tomchick D.R., Brautigam C.A., Moresco E.M.Y., Choi J.H.,
RA Beutler B.;
RT "Genetic and structural studies of RABL3 reveal an essential role in
RT lymphoid development and function.";
RL Proc. Natl. Acad. Sci. U.S.A. 117:8563-8572(2020).
CC -!- FUNCTION: Voltage dependent anion channel required for acidification
CC and functions of the Golgi apparatus that may function in counter-ion
CC conductance (By similarity). Plays a role in lymphocyte development,
CC probably by acting as a RABL3 effector in hematopoietic cells
CC (PubMed:32220963). {ECO:0000250|UniProtKB:B2ZXD5,
CC ECO:0000269|PubMed:32220963}.
CC -!- SUBUNIT: Homotrimer (By similarity). Interacts with RABL3; the
CC interaction stabilizes GPR89 (PubMed:32220963).
CC {ECO:0000250|UniProtKB:B7ZAQ6, ECO:0000269|PubMed:32220963}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:B2ZXD5}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the Golgi pH regulator (TC 1.A.38) family.
CC {ECO:0000305}.
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DR EMBL; AK034895; BAC28872.1; -; mRNA.
DR EMBL; AK016787; BAB30430.1; -; mRNA.
DR EMBL; BC010729; AAH10729.1; -; mRNA.
DR CCDS; CCDS17650.1; -.
DR RefSeq; NP_080505.1; NM_026229.4.
DR AlphaFoldDB; Q8BS95; -.
DR SMR; Q8BS95; -.
DR BioGRID; 212268; 1.
DR STRING; 10090.ENSMUSP00000029738; -.
DR GlyGen; Q8BS95; 1 site.
DR iPTMnet; Q8BS95; -.
DR PhosphoSitePlus; Q8BS95; -.
DR EPD; Q8BS95; -.
DR MaxQB; Q8BS95; -.
DR PaxDb; Q8BS95; -.
DR PeptideAtlas; Q8BS95; -.
DR PRIDE; Q8BS95; -.
DR ProteomicsDB; 271266; -.
DR DNASU; 67549; -.
DR Ensembl; ENSMUST00000029738; ENSMUSP00000029738; ENSMUSG00000028096.
DR GeneID; 67549; -.
DR KEGG; mmu:67549; -.
DR UCSC; uc008qom.1; mouse.
DR CTD; 67549; -.
DR MGI; MGI:1914799; Gpr89.
DR VEuPathDB; HostDB:ENSMUSG00000028096; -.
DR eggNOG; KOG2417; Eukaryota.
DR GeneTree; ENSGT00390000000684; -.
DR HOGENOM; CLU_030540_1_0_1; -.
DR InParanoid; Q8BS95; -.
DR OMA; HFNFYHR; -.
DR OrthoDB; 1065554at2759; -.
DR PhylomeDB; Q8BS95; -.
DR TreeFam; TF313484; -.
DR BioGRID-ORCS; 67549; 7 hits in 76 CRISPR screens.
DR ChiTaRS; Gpr89; mouse.
DR PRO; PR:Q8BS95; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q8BS95; protein.
DR Bgee; ENSMUSG00000028096; Expressed in small intestine Peyer's patch and 263 other tissues.
DR ExpressionAtlas; Q8BS95; baseline and differential.
DR Genevisible; Q8BS95; MM.
DR GO; GO:0032580; C:Golgi cisterna membrane; ISS:UniProtKB.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030660; C:Golgi-associated vesicle membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008308; F:voltage-gated anion channel activity; ISS:UniProtKB.
DR GO; GO:0051452; P:intracellular pH reduction; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0034765; P:regulation of ion transmembrane transport; IEA:UniProtKB-KW.
DR GO; GO:0043588; P:skin development; IMP:MGI.
DR GO; GO:0030217; P:T cell differentiation; IMP:UniProtKB.
DR InterPro; IPR025969; ABA_GPCR_dom.
DR InterPro; IPR022535; Golgi_pH-regulator_cons_dom.
DR InterPro; IPR015672; GPHR/GTG.
DR PANTHER; PTHR15948; PTHR15948; 1.
DR Pfam; PF12430; ABA_GPCR; 1.
DR Pfam; PF12537; GPHR_N; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Golgi apparatus; Ion channel; Ion transport; Membrane;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport; Voltage-gated channel.
FT CHAIN 1..455
FT /note="Golgi pH regulator"
FT /id="PRO_0000223261"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 378..398
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 180
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT MUTAGEN 273..303
FT /note="Missing: In explorer; no obvious external
FT deformities but mutants are 40% smaller than wild-type
FT litter mates and show reduced frequency of CD3+ T cells,
FT CD4+ T cells and CD8+ T cells which all display elevated
FT CD44 expression."
FT /evidence="ECO:0000269|PubMed:32220963"
FT CONFLICT 243
FT /note="N -> H (in Ref. 1; BAC28872)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 455 AA; 52735 MW; CAE9B93C64262252 CRC64;
MSFLIDSSIM ITSQILFFGF GWLFFMRQLF KDYEVRQYVV QVIFSVTFAF SCTMFELIIF
EILGVLNSSS RYFHWKLNLC VILLILVFMV PFYIGYFIVS NIQLLHKQRL LFSCLLWLTF
MYFFWKLGDP FPILSPKHGI LSIEQLISRV GVIGVTLMAL LSGFGAVNCP YTYMSYFLRN
VTDTDILALE RRLLQTMDMI ISKKKRMAVA RRTMFQRGDV QNKPSGLWGM LKSVTASAPG
SENLTLIQQE VDALEELSRQ LFLETADLYA TKERIEYSKT FKGKYFNFLG YFFSIYCVWK
IFMATINIVL DRVGKTDPVT RGIEITVNYL GIQFDVKFWS QHISFILVGI IIVTSIRGLL
ITLTKFFYAI SSSKSSNVIV LLLAQIMGMY FVSSVLLIRM SMPPEYRTII TEVLGELQFN
FYHRWFDVIF LVSALSSILF LYLAHKQAPE KHMAP
