GPI10_YEAST
ID GPI10_YEAST Reviewed; 616 AA.
AC P30777; D6VU07;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=GPI mannosyltransferase 3;
DE EC=2.4.1.-;
DE AltName: Full=GPI mannosyltransferase III;
DE Short=GPI-MT-III;
DE AltName: Full=Glycosylphosphatidylinositol-anchor biosynthesis protein 10;
GN Name=GPI10; OrderedLocusNames=YGL142C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=9046099;
RX DOI=10.1002/(sici)1097-0061(199702)13:2<177::aid-yea62>3.0.co;2-2;
RA Voet M., Defoor E., Verhasselt P., Riles L., Robben J., Volckaert G.;
RT "The sequence of a nearly unclonable 22.8 kb segment on the left arm
RT chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1,
RT MRF1 genes and six new open reading frames.";
RL Yeast 13:177-182(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 339-616.
RX PubMed=1475194; DOI=10.1093/nar/20.23.6339;
RA Pel H.J., Maat M.J., Rep M., Grivell L.A.;
RT "The yeast nuclear gene MRF1 encodes a mitochondrial peptide chain release
RT factor and cures several mitochondrial RNA splicing defects.";
RL Nucleic Acids Res. 20:6339-6346(1992).
RN [5]
RP FUNCTION.
RX PubMed=9576863; DOI=10.1042/bj3320153;
RA Suetterlin C., Escribano M.V., Gerold P., Maeda Y., Mazon M.J.,
RA Kinoshita T., Schwarz R.T., Riezman H.;
RT "Saccharomyces cerevisiae GPI10, the functional homologue of human PIG-B,
RT is required for glycosylphosphatidylinositol-anchor synthesis.";
RL Biochem. J. 332:153-159(1998).
RN [6]
RP FUNCTION, AND MUTAGENESIS OF ALA-48.
RX PubMed=9639537; DOI=10.1093/glycob/8.8.761;
RA Canivenc-Gansel E., Imhof I., Reggiori F., Burda P., Conzelmann A.,
RA Benachour A.;
RT "GPI anchor biosynthesis in yeast: phosphoethanolamine is attached to the
RT alpha1,4-linked mannose of the complete precursor glycophospholipid.";
RL Glycobiology 8:761-770(1998).
RN [7]
RP FUNCTION.
RX PubMed=11159918; DOI=10.1093/glycob/10.12.1271;
RA Imhof I., Canivenc-Gansel E., Meyer U., Conzelmann A.;
RT "Phosphatidylethanolamine is the donor of the phosphorylethanolamine linked
RT to the alpha1,4-linked mannose of yeast GPI structures.";
RL Glycobiology 10:1271-1275(2000).
RN [8]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Mannosyltransferase involved in glycosylphosphatidylinositol-
CC anchor biosynthesis. Transfers the third mannose to Man2-GlcN-acyl-PI
CC during GPI precursor assembly. {ECO:0000269|PubMed:11159918,
CC ECO:0000269|PubMed:9576863, ECO:0000269|PubMed:9639537}.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 22 family. PIGB
CC subfamily. {ECO:0000305}.
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DR EMBL; X99960; CAA68220.1; -; Genomic_DNA.
DR EMBL; Z72664; CAA96854.1; -; Genomic_DNA.
DR EMBL; X60381; CAA42931.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA07968.1; -; Genomic_DNA.
DR PIR; S64156; S64156.
DR RefSeq; NP_011373.1; NM_001181007.1.
DR AlphaFoldDB; P30777; -.
DR BioGRID; 33110; 449.
DR DIP; DIP-6707N; -.
DR IntAct; P30777; 2.
DR STRING; 4932.YGL142C; -.
DR CAZy; GT22; Glycosyltransferase Family 22.
DR MaxQB; P30777; -.
DR PaxDb; P30777; -.
DR PRIDE; P30777; -.
DR EnsemblFungi; YGL142C_mRNA; YGL142C; YGL142C.
DR GeneID; 852735; -.
DR KEGG; sce:YGL142C; -.
DR SGD; S000003110; GPI10.
DR VEuPathDB; FungiDB:YGL142C; -.
DR eggNOG; KOG1771; Eukaryota.
DR GeneTree; ENSGT00950000183090; -.
DR HOGENOM; CLU_012353_2_0_1; -.
DR InParanoid; P30777; -.
DR OMA; HEWPDYL; -.
DR BioCyc; YEAST:YGL142C-MON; -.
DR Reactome; R-SCE-162710; Synthesis of glycosylphosphatidylinositol (GPI).
DR UniPathway; UPA00196; -.
DR PRO; PR:P30777; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P30777; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:SGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000026; F:alpha-1,2-mannosyltransferase activity; IMP:SGD.
DR GO; GO:0004376; F:glycolipid mannosyltransferase activity; IEA:InterPro.
DR GO; GO:0000030; F:mannosyltransferase activity; IBA:GO_Central.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; IMP:SGD.
DR InterPro; IPR005599; GPI_mannosylTrfase.
DR InterPro; IPR039521; PIG-B/GPI10.
DR PANTHER; PTHR22760; PTHR22760; 1.
DR PANTHER; PTHR22760:SF4; PTHR22760:SF4; 1.
DR Pfam; PF03901; Glyco_transf_22; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Glycosyltransferase; GPI-anchor biosynthesis;
KW Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..616
FT /note="GPI mannosyltransferase 3"
FT /id="PRO_0000202736"
FT TOPO_DOM 1..16
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 38..86
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..136
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 158..188
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 210..240
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 262..278
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 300..338
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..359
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 360..392
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 414..423
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 424..444
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 445..616
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT MUTAGEN 48
FT /note="A->T: In gpi10-1; accumulate M2, an abnormal GPI-
FT anchor intermediate due to the absence of third mannose."
FT /evidence="ECO:0000269|PubMed:9639537"
FT CONFLICT 339
FT /note="S -> A (in Ref. 4; CAA42931)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 616 AA; 72566 MW; 141ABF6A01B008ED CRC64;
MAHEVHRIKP KLGRTQIFWV FLAFRVLNAV LTRTFFQADE FWQALEPAHW KAFKYGELTW
EWKFGVRSYL FPMIFELTYR LVSLSSILLH YALLLLSTIG SDLLILLLPK YELSWQVAED
LKRLPFDVTR SFEYYGVIYA PKIVMAVLAS IGEYYIVRFV QKLYLLTLDK RNEKEEEERR
SGLSEITKFA LLLSLTNFFN CFFITRTFIN SFEMILTSIA LYYWDWTGGQ MIKESSFTKS
LIFAFLACLQ RPSSGLIWVI PSISLILNLV GKKQYHLLFI TFSKVLRSFF LVFTANAIID
MYFYEKVTFP FFRFLKFNFT TPLSKFYGVA PWHFHFFQSL PIVLGASIPA FAFGLFFPLS
KRSFPKKYLN PFFQVKLTIL LNLLVYSTLP HKEFRFIFPL QPLFILISSF GLLRLDRDYW
KRLSGLKSLL WLVPFVSVFI ALLLDTFHES GSIEVMKFLH EEPEIDSLGF IMPCHSTPGQ
SYLHRSDIQD LWSITCNPPL HLLGDPEAYS KLETYMDESD HLYDDISAFI YKNFPPPFRK
DLRSPGKTYS HEWPTYLVVF EHMENAFLKD FLKDSSYIEY NRFFNSLAHW DSRRSGDIII
YYKLPFDYSD IPAADI
