GPI12_SCHPO
ID GPI12_SCHPO Reviewed; 248 AA.
AC Q9HDW9;
DT 11-JUL-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Probable N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase;
DE EC=3.5.1.89;
GN Name=gpi12; ORFNames=SPAPB2B4.01c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Involved in the second step of GPI biosynthesis. De-N-
CC acetylation of N-acetylglucosaminyl-phosphatidylinositol (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-
CC inositol + H2O = acetate + an alpha-D-GlcN-(1->6)-(1,2-diacyl-sn-
CC glycero-3-phospho)-1D-myo-inositol; Xref=Rhea:RHEA:11660,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:30089, ChEBI:CHEBI:57265,
CC ChEBI:CHEBI:57997; EC=3.5.1.89;
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:16823372}; Single-pass membrane protein
CC {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the PIGL family. {ECO:0000305}.
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DR EMBL; CU329670; CAC21467.1; -; Genomic_DNA.
DR RefSeq; NP_593887.1; NM_001019317.2.
DR AlphaFoldDB; Q9HDW9; -.
DR SMR; Q9HDW9; -.
DR STRING; 4896.SPAPB2B4.01c.1; -.
DR iPTMnet; Q9HDW9; -.
DR SwissPalm; Q9HDW9; -.
DR MaxQB; Q9HDW9; -.
DR PaxDb; Q9HDW9; -.
DR EnsemblFungi; SPAPB2B4.01c.1; SPAPB2B4.01c.1:pep; SPAPB2B4.01c.
DR GeneID; 2543490; -.
DR KEGG; spo:SPAPB2B4.01c; -.
DR PomBase; SPAPB2B4.01c; gpi12.
DR VEuPathDB; FungiDB:SPAPB2B4.01c; -.
DR eggNOG; KOG3332; Eukaryota.
DR HOGENOM; CLU_034979_0_1_1; -.
DR InParanoid; Q9HDW9; -.
DR OMA; RWGWITI; -.
DR PhylomeDB; Q9HDW9; -.
DR Reactome; R-SPO-162710; Synthesis of glycosylphosphatidylinositol (GPI).
DR UniPathway; UPA00196; -.
DR PRO; PR:Q9HDW9; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:PomBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISO:PomBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IBA:GO_Central.
DR GO; GO:0000225; F:N-acetylglucosaminylphosphatidylinositol deacetylase activity; ISO:PomBase.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; ISO:PomBase.
DR Gene3D; 3.40.50.10320; -; 1.
DR InterPro; IPR003737; GlcNAc_PI_deacetylase-related.
DR InterPro; IPR039516; GPI12.
DR InterPro; IPR024078; LmbE-like_dom_sf.
DR PANTHER; PTHR12993; PTHR12993; 1.
DR PANTHER; PTHR12993:SF11; PTHR12993:SF11; 1.
DR Pfam; PF02585; PIG-L; 1.
DR SUPFAM; SSF102588; SSF102588; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; GPI-anchor biosynthesis; Hydrolase; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..248
FT /note="Probable N-acetylglucosaminyl-phosphatidylinositol
FT de-N-acetylase"
FT /id="PRO_0000207169"
FT TOPO_DOM 1..7
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 8..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 25..248
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 248 AA; 28006 MW; 4DD8E6DF5DCFD1FF CRC64;
MIWFWSTLLV TAIAVLSTAN ESSSGQEKLA VESILFVFAH PDDESMFFGP TIDYLGNQHS
TRVHVLCLSN GNADGLGSVR EKELVVAASK YQIDKTNVHV VSDPQLQDGM QAKWDPTDVA
KHISQIIERY NIKTLITFDN KGISGHPNHI ACYEGAMKIV KATPQVQVFV LESVNIFRKY
ISYLDTIPTL VQSQAGRNDT IIIHADRKST QRIRDAMVRG HKSQMVWFRY GWIYLSKYMS
NNVLKRAT
