GPI16_SCHPO
ID GPI16_SCHPO Reviewed; 545 AA.
AC O94380;
DT 01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=GPI transamidase component PIG-T homolog;
DE Flags: Precursor;
GN Name=gpi16; ORFNames=SPBC1604.15;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Component of the GPI transamidase complex. Involved in
CC transfer of GPI to proteins (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis.
CC -!- SUBUNIT: Forms a complex with PIG-S homolog, PIG-U homolog and GPI8.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:16823372}; Single-pass type I membrane protein
CC {ECO:0000269|PubMed:16823372}.
CC -!- PTM: The disulfide bond between PIGK/GPI8 and PIGT is important for
CC normal enzyme activity. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PIGT family. {ECO:0000305}.
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DR EMBL; CU329671; CAA22348.1; -; Genomic_DNA.
DR PIR; T39499; T39499.
DR RefSeq; NP_596625.1; NM_001022546.2.
DR AlphaFoldDB; O94380; -.
DR SMR; O94380; -.
DR STRING; 4896.SPBC1604.15.1; -.
DR MaxQB; O94380; -.
DR PaxDb; O94380; -.
DR EnsemblFungi; SPBC1604.15.1; SPBC1604.15.1:pep; SPBC1604.15.
DR GeneID; 2539759; -.
DR KEGG; spo:SPBC1604.15; -.
DR PomBase; SPBC1604.15; gpi16.
DR VEuPathDB; FungiDB:SPBC1604.15; -.
DR eggNOG; KOG2407; Eukaryota.
DR HOGENOM; CLU_021459_2_1_1; -.
DR InParanoid; O94380; -.
DR OMA; LWAWIDA; -.
DR PhylomeDB; O94380; -.
DR UniPathway; UPA00196; -.
DR PRO; PR:O94380; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:PomBase.
DR GO; GO:0042765; C:GPI-anchor transamidase complex; ISO:PomBase.
DR GO; GO:0016255; P:attachment of GPI anchor to protein; ISO:PomBase.
DR InterPro; IPR007245; PIG-T.
DR PANTHER; PTHR12959; PTHR12959; 1.
DR Pfam; PF04113; Gpi16; 1.
PE 3: Inferred from homology;
KW Disulfide bond; Endoplasmic reticulum; Glycoprotein;
KW GPI-anchor biosynthesis; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..545
FT /note="GPI transamidase component PIG-T homolog"
FT /id="PRO_0000024109"
FT TOPO_DOM 23..493
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 494..514
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 515..545
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 168
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 227
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 336
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 391
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 467
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 182
FT /note="Interchain (with C-73 in PIGK/GPI8)"
FT /evidence="ECO:0000250"
SQ SEQUENCE 545 AA; 62065 MW; DD2C36016ABF4A9D CRC64;
MKKNGCLLLF AYSLLSFSLT AATIDETYDE SLFIKSFSSR YSYVSFAFEI GASTDSTHSS
VFSESSFSLF PLSIARVMDE CQVSELHIRA TRGRWDYENW KESPDNGFYS GGLGFEVWAF
MANDPSMKYW LKLTNQLSGL LCASLNYIDS SNTYQPQLSY PGSFSFSNNT QYFASLPQED
VCTENLSPLF KLLPCKRKAG IASLLDSHLF FDTDWHSFSI DVYPSENQSL ASVKMGIIIQ
AVVDVERNGR RKGKTTFQPP SEYCHDEDMD SLHCLMSGYS TEHHTVDDLF HKVPKERCLL
SSTFSDVFVS NGDKIDTFSL DEAANIQIPI QSTSDNHTVT VDRSLSNDGN HWGSLSSTIY
NPSSSPRTIV YFEKFPWFVR VYLHTLTITL NGTRINTKDF IEKLYYQPLR DRKAGTMMEI
QFSIPPHTNL IVHFNVEKTP LRLDEYPPDA NRGYNLPPAI ISVFDENNTK LCSLRTAALL
MFIPTPDFSM PYNVIIFTST VIALTFGGIF NLLTRRFVPQ QSKFQNRQPS MLQRLKEKIF
HKKRG
