GPI1_SCHPO
ID GPI1_SCHPO Reviewed; 653 AA.
AC O14357; P78971;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 128.
DE RecName: Full=N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein gpi1;
GN Name=gpi1; ORFNames=SPBC30D10.11;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9046095;
RX DOI=10.1002/(sici)1097-0061(199702)13:2<139::aid-yea69>3.0.co;2-s;
RA Colussi P.A., Orlean P.;
RT "The essential Schizosaccharomyces pombe gpi1+ gene complements a bakers'
RT yeast GPI anchoring mutant and is required for efficient cell separation.";
RL Yeast 13:139-150(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Necessary for the synthesis of N-acetylglucosaminyl-
CC phosphatidylinositol, the very early intermediate in GPI-anchor
CC biosynthesis.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC49650.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U77355; AAC49650.1; ALT_INIT; mRNA.
DR EMBL; CU329671; CAB10806.1; -; Genomic_DNA.
DR PIR; T40185; T40185.
DR RefSeq; NP_596274.1; NM_001022195.2.
DR AlphaFoldDB; O14357; -.
DR STRING; 4896.SPBC30D10.11.1; -.
DR PaxDb; O14357; -.
DR EnsemblFungi; SPBC30D10.11.1; SPBC30D10.11.1:pep; SPBC30D10.11.
DR GeneID; 2540323; -.
DR KEGG; spo:SPBC30D10.11; -.
DR PomBase; SPBC30D10.11; gpi1.
DR VEuPathDB; FungiDB:SPBC30D10.11; -.
DR eggNOG; KOG1183; Eukaryota.
DR HOGENOM; CLU_007914_2_1_1; -.
DR InParanoid; O14357; -.
DR OMA; YNWQLTI; -.
DR PhylomeDB; O14357; -.
DR Reactome; R-SPO-162710; Synthesis of glycosylphosphatidylinositol (GPI).
DR UniPathway; UPA00196; -.
DR PRO; PR:O14357; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:PomBase.
DR GO; GO:0000506; C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex; ISO:PomBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0017176; F:phosphatidylinositol N-acetylglucosaminyltransferase activity; IGI:PomBase.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; IGI:PomBase.
DR InterPro; IPR007720; PigQ/GPI1.
DR PANTHER; PTHR21329; PTHR21329; 1.
DR Pfam; PF05024; Gpi1; 1.
PE 2: Evidence at transcript level;
KW GPI-anchor biosynthesis; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..653
FT /note="N-acetylglucosaminyl-phosphatidylinositol
FT biosynthetic protein gpi1"
FT /id="PRO_0000087557"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 382..402
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 485..505
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 520..540
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 605..625
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 448
FT /note="L -> M (in Ref. 1; AAC49650)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 653 AA; 76432 MW; 916CF84ADEA0C66E CRC64;
MSTTTMNIAS APIEVKRIYW PKSTISYTDS GYLVGWKYST NDLIVVTTIT SYQSFANFLV
DYCKNQDQSV DIKSLCILGT FNCSADILPG DKYEIDCWIK VQQSTELSHP RVFDSASTPL
KINLHIIYYH PPQPRKMQFL SLEPLSLLLL KDSFINKSNP EYESMQHQQI LLKKLKLHFP
RRKENSWKRS LRSGLIELLN QSFEVRMLTH ENNNKKNSYV FRLFDRVSSS TFYFFNSLFA
YFIILLRIIN EVILLAINYR PIPLSYNMMD IFVSARQVDL RLQQACFWPV QYMKLWVFRK
SKRVAIEDYK EYIRFYNNLW LVANDMIFGI TMSSFILENL HLVVKLIENI TFEYAIKNVR
SMVIWLVDTP AGLKLNNDIC KFIMKLSVWV IDVWSNFLLH CLPWTPFLVQ VVAISGFGGA
SLMIALISDF LSVMTIHIHL LYLASSRLYN WQLRVIYSLL QLFRGKKRNV LRNRIDSYEY
DLDQLLLGTI LFTVLIFFLP TIYVFYAAFA LTRVSVMTCL AICETMLAFL NHFPLFVTML
RIKDPYRIPS GLNFEIVSFE PLKQDGFATL YLNCNSKPMS LGSMFEHYRK LARRLISHYL
SKTTLISLLV GCPVPAIPAE QLYNIQYAML PTKRISIRKL RDLLFHQKKF PYD
