GPKOW_MOUSE
ID GPKOW_MOUSE Reviewed; 488 AA.
AC Q56A08; A0JLW7; A2AEU7; Q6P5V0; Q8C1C3;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 2.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=G-patch domain and KOW motifs-containing protein;
DE AltName: Full=Protein MOS2 homolog;
GN Name=Gpkow;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Liver;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-488.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION.
RX PubMed=16271871; DOI=10.1016/j.cub.2005.09.038;
RA Zhang Y., Cheng Y.T., Bi D., Palma K., Li X.;
RT "MOS2, a protein containing G-patch and KOW motifs, is essential for innate
RT immunity in Arabidopsis thaliana.";
RL Curr. Biol. 15:1936-1942(2005).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-485, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, and Liver;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: RNA-binding protein involved in pre-mRNA splicing.
CC {ECO:0000250|UniProtKB:Q92917}.
CC -!- SUBUNIT: Interacts with PRKX, PRKACB and DHX16.
CC {ECO:0000250|UniProtKB:Q92917}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q92917}.
CC -!- PTM: Phosphorylation regulates its ability to bind RNA.
CC {ECO:0000250|UniProtKB:Q92917}.
CC -!- SIMILARITY: Belongs to the MOS2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH92224.2; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAI20680.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAI25336.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAC25949.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK028433; BAC25949.1; ALT_INIT; mRNA.
DR EMBL; AL671995; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC062646; AAH62646.1; -; mRNA.
DR EMBL; BC092224; AAH92224.2; ALT_INIT; mRNA.
DR EMBL; BC120679; AAI20680.1; ALT_INIT; mRNA.
DR EMBL; BC125335; AAI25336.1; ALT_INIT; mRNA.
DR CCDS; CCDS29969.2; -.
DR RefSeq; NP_776108.2; NM_173747.3.
DR AlphaFoldDB; Q56A08; -.
DR SMR; Q56A08; -.
DR BioGRID; 229077; 14.
DR IntAct; Q56A08; 13.
DR STRING; 10090.ENSMUSP00000076178; -.
DR iPTMnet; Q56A08; -.
DR PhosphoSitePlus; Q56A08; -.
DR EPD; Q56A08; -.
DR jPOST; Q56A08; -.
DR MaxQB; Q56A08; -.
DR PaxDb; Q56A08; -.
DR PeptideAtlas; Q56A08; -.
DR PRIDE; Q56A08; -.
DR ProteomicsDB; 271267; -.
DR Antibodypedia; 340; 181 antibodies from 28 providers.
DR DNASU; 209416; -.
DR Ensembl; ENSMUST00000049896; ENSMUSP00000076178; ENSMUSG00000031148.
DR GeneID; 209416; -.
DR KEGG; mmu:209416; -.
DR UCSC; uc009sma.1; mouse.
DR CTD; 27238; -.
DR MGI; MGI:1859610; Gpkow.
DR VEuPathDB; HostDB:ENSMUSG00000031148; -.
DR eggNOG; KOG4315; Eukaryota.
DR GeneTree; ENSGT00390000015154; -.
DR HOGENOM; CLU_045183_1_0_1; -.
DR InParanoid; Q56A08; -.
DR OMA; VEGNRVM; -.
DR OrthoDB; 1427830at2759; -.
DR PhylomeDB; Q56A08; -.
DR TreeFam; TF316786; -.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR BioGRID-ORCS; 209416; 25 hits in 75 CRISPR screens.
DR ChiTaRS; Gpkow; mouse.
DR PRO; PR:Q56A08; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q56A08; protein.
DR Bgee; ENSMUSG00000031148; Expressed in manus and 220 other tissues.
DR ExpressionAtlas; Q56A08; baseline and differential.
DR Genevisible; Q56A08; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005681; C:spliceosomal complex; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR CDD; cd13152; KOW_GPKOW_A; 1.
DR CDD; cd13153; KOW_GPKOW_B; 1.
DR Gene3D; 2.30.30.30; -; 2.
DR InterPro; IPR000467; G_patch_dom.
DR InterPro; IPR041993; GPKOW_KOW1.
DR InterPro; IPR041994; GPKOW_KOW2.
DR InterPro; IPR005824; KOW.
DR InterPro; IPR014722; Rib_L2_dom2.
DR InterPro; IPR045166; Spp2-like.
DR InterPro; IPR026822; Spp2/MOS2_G-patch.
DR InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR PANTHER; PTHR15818; PTHR15818; 1.
DR Pfam; PF12656; G-patch_2; 1.
DR Pfam; PF00467; KOW; 1.
DR SMART; SM00443; G_patch; 1.
DR SMART; SM00739; KOW; 2.
DR SUPFAM; SSF50104; SSF50104; 1.
DR PROSITE; PS50174; G_PATCH; 1.
PE 1: Evidence at protein level;
KW Acetylation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; RNA-binding.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q92917"
FT CHAIN 2..488
FT /note="G-patch domain and KOW motifs-containing protein"
FT /id="PRO_0000280563"
FT DOMAIN 164..210
FT /note="G-patch"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00092"
FT DOMAIN 231..258
FT /note="KOW 1"
FT DOMAIN 401..428
FT /note="KOW 2"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 72..97
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 216..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 295..367
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 75..97
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 223..238
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 295..329
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 330..367
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q92917"
FT MOD_RES 25
FT /note="Phosphoserine; by PKA"
FT /evidence="ECO:0000250|UniProtKB:Q92917"
FT MOD_RES 115
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q92917"
FT MOD_RES 485
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 488 AA; 53832 MW; BCF1E648CA7CDD4B CRC64;
MAGRESPPPS APSMAPISFG FTRTSVRRRL ADLGDSERQA PEEKDFLATV EGRKLQSVNP
PEAPKELVIP LIQNGSRRQP LSKNPKPSSE TSTVLMSDGV LSQAVKELIE ESKKSLEERE
NAGVDPTLTI PMIQKGCTPI EEGSDSEPQA ETVPEEADYE AVPVEAYGLA MLRGMGWKPG
KGIGNTFSQV VKPRVNSIRP KGLGLGANRM EAQDLASVGS HHPPRPDGDR ENDKEGQPQG
LMHGRAVVVL SGPYRGLYGK VEGLDPDNVR AMVRLAVGNR IVTVSEYCLR PVSQQEFDSH
TSKPGHVSQT STEQQNRATG TASSLKAAQN QEDSKRRQKG SEKKRKHSPD RQDGPVPKTE
KAAPRNKHWL HRDLRVRFID KLHKSGRYYN TKMTIEDVLS PDTCVCRTDE GRVLEDVRED
MLETLIPKGE GHRVMVVLGP HAGKVGLLRS RDRAQSHALV QLRRENQVVE LHYNAICQYM
GPGDSDED
