GPN2_DICDI
ID GPN2_DICDI Reviewed; 315 AA.
AC Q54TE7;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=GPN-loop GTPase 2 homolog {ECO:0000250|UniProtKB:Q9H9Y4};
DE AltName: Full=ATP-binding domain 1 family member B homolog {ECO:0000250|UniProtKB:Q9H9Y4};
GN Name=gpn2 {ECO:0000312|dictyBase:DDB_G0281787};
GN Synonyms=atpbd1b {ECO:0000250|UniProtKB:Q9H9Y4}; ORFNames=DDB_G0281787;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Small GTPase required for proper localization of RNA
CC polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly
CC step prior to nuclear import. {ECO:0000250|UniProtKB:Q08726}.
CC -!- SUBUNIT: Heterodimers with gpn1 or gpn3. Binds to RNA polymerase II
CC (RNAPII). {ECO:0000250|UniProtKB:Q08726, ECO:0000250|UniProtKB:Q9H9Y4}.
CC -!- SIMILARITY: Belongs to the GPN-loop GTPase family. {ECO:0000305}.
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DR EMBL; AAFI02000042; EAL66655.1; -; Genomic_DNA.
DR RefSeq; XP_640644.1; XM_635552.1.
DR AlphaFoldDB; Q54TE7; -.
DR SMR; Q54TE7; -.
DR STRING; 44689.DDB0233479; -.
DR PaxDb; Q54TE7; -.
DR PRIDE; Q54TE7; -.
DR EnsemblProtists; EAL66655; EAL66655; DDB_G0281787.
DR GeneID; 8623255; -.
DR KEGG; ddi:DDB_G0281787; -.
DR dictyBase; DDB_G0281787; gpn2.
DR eggNOG; KOG1533; Eukaryota.
DR HOGENOM; CLU_037460_0_2_1; -.
DR InParanoid; Q54TE7; -.
DR OMA; YAPLPFN; -.
DR PhylomeDB; Q54TE7; -.
DR PRO; PR:Q54TE7; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR004130; Gpn.
DR InterPro; IPR030231; Gpn2.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR21231; PTHR21231; 1.
DR PANTHER; PTHR21231:SF3; PTHR21231:SF3; 1.
DR Pfam; PF03029; ATP_bind_1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 2: Evidence at transcript level;
KW GTP-binding; Hydrolase; Nucleotide-binding; Reference proteome.
FT CHAIN 1..315
FT /note="GPN-loop GTPase 2 homolog"
FT /id="PRO_0000330933"
FT MOTIF 69..71
FT /note="Gly-Pro-Asn (GPN)-loop; involved in dimer interface"
FT /evidence="ECO:0000250|UniProtKB:Q9UYR9"
FT BINDING 12..17
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q9UYR9"
FT BINDING 171..174
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q9UYR9"
FT SITE 71
FT /note="Stabilizes the phosphate intermediate; shared with
FT dimeric partner"
FT /evidence="ECO:0000250|UniProtKB:Q9UYR9"
SQ SEQUENCE 315 AA; 36364 MW; D72AA1586CC5A614 CRC64;
MGFGQVVIGP PGSGKTVYCN GMSQFLQSIG RKVSIINLDP SNENIPYEPA VNIQELIDFQ
TVVNETDLGP NGGLIFCMEY LEKNLDWLKE KLLPLKDHYI IFDCPGQVEL YTHYKIISNI
LDNIMKWSFR LTVIQVFDSF YCKNPSNFIS ILLVSLSGMV RIELPHINVL SKMDLIEQNG
PLDFNLDFYT DVLDLKYLDA FLDKDPRLKK YSKLNKAIAG VIEDFSLVSF IPLNIMDKKS
VANLIASIDK SNGYIYGSLD TNTAILEIQE RETQWNFDKY QETQEKYYKS YEDDDVIFEN
DQDEDYDEFS KYLNR
