ID   GPN2_XENLA              Reviewed;         318 AA.
AC   Q66KF6; Q7ZYS2;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 59.
DE   RecName: Full=GPN-loop GTPase 2 {ECO:0000250|UniProtKB:Q9H9Y4};
DE   AltName: Full=ATP-binding domain 1 family member B {ECO:0000250|UniProtKB:Q9H9Y4};
GN   Name=gpn2 {ECO:0000250|UniProtKB:Q9H9Y4};
GN   Synonyms=atpbd1b {ECO:0000250|UniProtKB:Q9H9Y4};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Small GTPase required for proper localization of RNA
CC       polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly
CC       step prior to nuclear import. {ECO:0000250|UniProtKB:Q08726}.
CC   -!- SUBUNIT: Heterodimers with gpn1 or gpn3. Binds to RNA polymerase II
CC       (RNAPII). {ECO:0000250|UniProtKB:Q08726, ECO:0000250|UniProtKB:Q9H9Y4}.
CC   -!- SIMILARITY: Belongs to the GPN-loop GTPase family. {ECO:0000305}.
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DR   EMBL; BC041519; AAH41519.1; -; mRNA.
DR   EMBL; BC080422; AAH80422.1; -; mRNA.
DR   RefSeq; NP_001082424.1; NM_001088955.1.
DR   AlphaFoldDB; Q66KF6; -.
DR   SMR; Q66KF6; -.
DR   BioGRID; 99792; 1.
DR   IntAct; Q66KF6; 1.
DR   DNASU; 398460; -.
DR   GeneID; 398460; -.
DR   KEGG; xla:398460; -.
DR   CTD; 398460; -.
DR   Xenbase; XB-GENE-5751928; gpn2.S.
DR   OrthoDB; 964931at2759; -.
DR   Proteomes; UP000186698; Chromosome 2S.
DR   Bgee; 398460; Expressed in egg cell and 19 other tissues.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR004130; Gpn.
DR   InterPro; IPR030231; Gpn2.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21231; PTHR21231; 1.
DR   PANTHER; PTHR21231:SF3; PTHR21231:SF3; 1.
DR   Pfam; PF03029; ATP_bind_1; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   2: Evidence at transcript level;
KW   GTP-binding; Hydrolase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..318
FT                   /note="GPN-loop GTPase 2"
FT                   /id="PRO_0000247833"
FT   MOTIF           85..87
FT                   /note="Gly-Pro-Asn (GPN)-loop; involved in dimer interface"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UYR9"
FT   BINDING         29..34
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UYR9"
FT   BINDING         187..190
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UYR9"
FT   SITE            87
FT                   /note="Stabilizes the phosphate intermediate; shared with
FT                   dimeric partner"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UYR9"
SQ   SEQUENCE   318 AA;  35346 MW;  D34B317B808C4983 CRC64;
     MADACSEMDR GAEKYPLLGF GQAVIGPPGS GKSTYVRAMQ ALLARMGRKS AIINLDPAGE
     DEPGAAVSLR ELLGLEEVMS ELRLGPNGSL LYCMEYLQEN LDWLRARLQG LRGTYLLLDC
     PGQVELYTHH PALPDILRRL GGWGLRLCAV HLVDSHYCTD PAKFISVLCT SLSTMLHVEL
     PHINVLSKMD LIEQYGRLAF NLDYYTEVMD LSFLVEQLTS DPFFRRHKRL HEKLAGVIED
     YGLVTFMPLS IKDDKSLQLV LSAVDKASGF CFGEAKQSLG NLMSVAVGAD FQFTSTLAFQ
     EKYVENDGRT VEEETLDL