ID   GPN3_KLULA              Reviewed;         271 AA.
AC   Q6CQA6;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 2.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=GPN-loop GTPase 3 {ECO:0000250|UniProtKB:Q06543};
GN   OrderedLocusNames=KLLA0D18557g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Small GTPase required for proper nuclear import of RNA
CC       polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly
CC       step prior to nuclear import. {ECO:0000250|UniProtKB:Q06543}.
CC   -!- SUBUNIT: Heterodimers with GPN1 or GPN2. Binds to RNA polymerase II
CC       (RNAPII). {ECO:0000250|UniProtKB:Q06543}.
CC   -!- SIMILARITY: Belongs to the GPN-loop GTPase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAH00979.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CR382124; CAH00979.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_453883.1; XM_453883.1.
DR   AlphaFoldDB; Q6CQA6; -.
DR   SMR; Q6CQA6; -.
DR   STRING; 28985.XP_453883.1; -.
DR   EnsemblFungi; CAH00979; CAH00979; KLLA0_D18557g.
DR   GeneID; 2893468; -.
DR   KEGG; kla:KLLA0_D18557g; -.
DR   eggNOG; KOG1534; Eukaryota.
DR   HOGENOM; CLU_037460_0_0_1; -.
DR   InParanoid; Q6CQA6; -.
DR   Proteomes; UP000000598; Chromosome D.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0007064; P:mitotic sister chromatid cohesion; IEA:EnsemblFungi.
DR   GO; GO:0006606; P:protein import into nucleus; IEA:EnsemblFungi.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR004130; Gpn.
DR   InterPro; IPR030228; Gpn3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21231; PTHR21231; 1.
DR   PANTHER; PTHR21231:SF7; PTHR21231:SF7; 1.
DR   Pfam; PF03029; ATP_bind_1; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Hydrolase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..271
FT                   /note="GPN-loop GTPase 3"
FT                   /id="PRO_0000255593"
FT   MOTIF           70..72
FT                   /note="Gly-Pro-Asn (GPN)-loop; involved in dimer interface"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UYR9"
FT   BINDING         13..18
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UYR9"
FT   BINDING         173..176
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UYR9"
FT   SITE            72
FT                   /note="Stabilizes the phosphate intermediate; shared with
FT                   dimeric partner"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UYR9"
SQ   SEQUENCE   271 AA;  30473 MW;  0F6AF038DB53C085 CRC64;
     MSRVGVLVLG PAGAGKSTFC NAIISHMQSI GRRAHIVNLD PAAEATKYEF TIDIRDLISL
     EDVMEEFGLG PNGSLIYCFE YLLNNLDWLD EEIGDYNDEY LIFDCPGQIE LYTHIPVLPT
     IVRHLQNQLN FNLCATYLLE APFVIDTSKF FSGALSAMSA MILLELPHIN ILSKLDLVKD
     SHNKKALKKF LNPDPLLLTD KVNEETNPKF HKLNEAIANL VDDFGMVQFL PLEAKNPESV
     STILSYIDDV TQWAEAQEPK EPNDQIEIDD M