GPN3_SCHPO
ID GPN3_SCHPO Reviewed; 276 AA.
AC O14443;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=GPN-loop GTPase 3 {ECO:0000250|UniProtKB:Q06543};
DE EC=3.6.5.- {ECO:0000250|UniProtKB:Q06543};
DE AltName: Full=Factor of eukaryotic transcription 5 {ECO:0000303|PubMed:9190794};
GN Name=fet5 {ECO:0000303|PubMed:9190794};
GN ORFNames=SPAC4D7.12c {ECO:0000312|PomBase:SPAC4D7.12c};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=9190794;
RA Shpakovskii G.V., Lebedenko E.N.;
RT "The first member of a novel family of eukaryotic transcription factors
RT detected by the heterospecific complementation.";
RL Bioorg. Khim. 23:234-237(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=9551201;
RA Shpakovskii G.V., Lebedenko E.N.;
RT "Exon-intron structure of the fet5+ gene of Schizosaccharomyces pombe and
RT physical mapping of genome encompassing regions.";
RL Bioorg. Khim. 24:42-47(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Small GTPase required for proper nuclear import of RNA
CC polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly
CC step prior to nuclear import. {ECO:0000250|UniProtKB:Q06543}.
CC -!- SUBUNIT: Heterodimers with gpn1 or gpn2. Binds to RNA polymerase II
CC (RNAPII). {ECO:0000250|UniProtKB:Q06543}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the GPN-loop GTPase family. {ECO:0000305}.
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DR EMBL; U80218; AAC49837.1; -; mRNA.
DR EMBL; AF020907; AAD01680.1; -; Genomic_DNA.
DR EMBL; CU329670; CAB11284.1; -; Genomic_DNA.
DR PIR; T43541; T43541.
DR RefSeq; NP_594965.1; NM_001020396.2.
DR AlphaFoldDB; O14443; -.
DR SMR; O14443; -.
DR STRING; 4896.SPAC4D7.12c.1; -.
DR MaxQB; O14443; -.
DR PaxDb; O14443; -.
DR EnsemblFungi; SPAC4D7.12c.1; SPAC4D7.12c.1:pep; SPAC4D7.12c.
DR GeneID; 2543397; -.
DR KEGG; spo:SPAC4D7.12c; -.
DR PomBase; SPAC4D7.12c; fet5.
DR VEuPathDB; FungiDB:SPAC4D7.12c; -.
DR eggNOG; KOG1534; Eukaryota.
DR HOGENOM; CLU_037460_0_0_1; -.
DR InParanoid; O14443; -.
DR OMA; CFEYLLQ; -.
DR PhylomeDB; O14443; -.
DR PRO; PR:O14443; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; ISM:PomBase.
DR GO; GO:0006606; P:protein import into nucleus; ISO:PomBase.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR004130; Gpn.
DR InterPro; IPR030228; Gpn3.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR21231; PTHR21231; 1.
DR PANTHER; PTHR21231:SF7; PTHR21231:SF7; 1.
DR Pfam; PF03029; ATP_bind_1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; GTP-binding; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..276
FT /note="GPN-loop GTPase 3"
FT /id="PRO_0000255594"
FT REGION 257..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 70..72
FT /note="Gly-Pro-Asn (GPN)-loop; involved in dimer interface"
FT /evidence="ECO:0000250|UniProtKB:Q9UYR9"
FT BINDING 13..18
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q9UYR9"
FT BINDING 173..176
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q9UYR9"
FT SITE 72
FT /note="Stabilizes the phosphate intermediate; shared with
FT dimeric partner"
FT /evidence="ECO:0000250|UniProtKB:Q9UYR9"
SQ SEQUENCE 276 AA; 31537 MW; 13E4A4C0FC26DBC4 CRC64;
MVKVAAFVCG VASSGKSTFC GALMSYMKSV GRSCHLVNLD PAAENFEWEP TVDIRDLISI
DDVMEELDYG PNGGLIYCFE FLMENLDWLN EEIGDYDEDY LIFDMPGQIE LYTHVPILPA
LIRHLQVTLN FRPCAVYLLE SQFLVDRTKF FAGVLSAMSA MVMMEVPHIN LLSKMDLLKD
NNNITKAELK RFLNTDPLLL TGEINETTNP KFHELNRCIV QLIDDFNMVN FLPLESGNEE
SVSRVLSYID DATQWYEDQE PKDPDRFEAD DLEDDE
