ID   GPPA_VIBC3              Reviewed;         497 AA.
AC   A5F3R3; C3M3W5;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_01550};
DE            EC=3.6.1.40 {ECO:0000255|HAMAP-Rule:MF_01550};
DE   AltName: Full=Guanosine pentaphosphate phosphohydrolase {ECO:0000255|HAMAP-Rule:MF_01550};
DE   AltName: Full=pppGpp-5'-phosphohydrolase {ECO:0000255|HAMAP-Rule:MF_01550};
GN   Name=gppA {ECO:0000255|HAMAP-Rule:MF_01550};
GN   OrderedLocusNames=VC0395_A2696, VC395_0348;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA   Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the cholera
RT   pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
CC   -!- FUNCTION: Catalyzes the conversion of pppGpp to ppGpp. Guanosine
CC       pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which
CC       together with ppGpp controls the 'stringent response', an adaptive
CC       process that allows bacteria to respond to amino acid starvation,
CC       resulting in the coordinated regulation of numerous cellular
CC       activities. {ECO:0000255|HAMAP-Rule:MF_01550}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine 3'-diphosphate 5'-triphosphate + H2O = guanosine
CC         3',5'-bis(diphosphate) + H(+) + phosphate; Xref=Rhea:RHEA:13073,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:77828, ChEBI:CHEBI:142410; EC=3.6.1.40;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01550};
CC   -!- PATHWAY: Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step
CC       2/2. {ECO:0000255|HAMAP-Rule:MF_01550}.
CC   -!- SIMILARITY: Belongs to the GppA/Ppx family. GppA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01550}.
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DR   EMBL; CP000627; ABQ19640.1; -; Genomic_DNA.
DR   EMBL; CP001235; ACP08373.1; -; Genomic_DNA.
DR   RefSeq; WP_000076046.1; NZ_JAACZH010000020.1.
DR   AlphaFoldDB; A5F3R3; -.
DR   SMR; A5F3R3; -.
DR   STRING; 345073.VC395_0348; -.
DR   EnsemblBacteria; ABQ19640; ABQ19640; VC0395_A2696.
DR   GeneID; 57739029; -.
DR   KEGG; vco:VC0395_A2696; -.
DR   KEGG; vcr:VC395_0348; -.
DR   PATRIC; fig|345073.21.peg.335; -.
DR   eggNOG; COG0248; Bacteria.
DR   HOGENOM; CLU_025908_4_0_6; -.
DR   OMA; WQICVGA; -.
DR   UniPathway; UPA00908; UER00885.
DR   Proteomes; UP000000249; Chromosome 2.
DR   GO; GO:0008894; F:guanosine-5'-triphosphate,3'-diphosphate diphosphatase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015974; P:guanosine pentaphosphate catabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0015970; P:guanosine tetraphosphate biosynthetic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_01550; GppA; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR023709; Guo-5TP_3DP_PyrP.
DR   InterPro; IPR003695; Ppx_GppA.
DR   InterPro; IPR030673; PyroPPase_GppA_Ppx.
DR   Pfam; PF02541; Ppx-GppA; 1.
DR   PIRSF; PIRSF001267; Pyrophosphatase_GppA_Ppx; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
PE   3: Inferred from homology;
KW   Hydrolase.
FT   CHAIN           1..497
FT                   /note="Guanosine-5'-triphosphate,3'-diphosphate
FT                   pyrophosphatase"
FT                   /id="PRO_1000073559"
SQ   SEQUENCE   497 AA;  54826 MW;  BB0BD17FF3B3A331 CRC64;
     MSQAVSSPLY AAIDLGSNSF HMLVVRHIDG SVQTMAKIKR KVRLAAGLDE HNALSLDAMQ
     RGWDCLSLFA ERLQDIPAEN IRIVGTATLR TATNAGEFIA KANQILGHPI DVISGEEEAA
     TIYKGVAHTS GGLGRRLVVD IGGASTELII GEGFEAKALT SLKMGCVTWL ERHFKDRQLT
     ATNFNNAILA AKQMLDPILT QYTELGWNVC VGASGTVQAL QEIMLAQGMD EVITLTKLKR
     LQKQAMLADH LEELDIEGLT LERALVFPSG LSILIAIFES LNIEAMTLAG GALREGLVYE
     MVQDLRQEDI RARTIRCVQT RYQIDSAYGD QVATLASKLL AQCGGEAWIN EPQAEMLLRT
     AAKLHEIGLT IDFKKGGEHS AYLLQHLDLP GYTRAQKHYL GEIVRRYREQ LTSLPEQYAL
     SGTSGKRVLR LLRLAVLLSH RRSPALEPMV ELSAQEDKLT LTLDGEWLAK NPLTRTELEL
     EANRQTDIGW PLSIECH