ID   GPPMT_SACEN             Reviewed;         285 AA.
AC   A4FG18;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   17-APR-2007, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Geranyl diphosphate 2-C-methyltransferase;
DE            Short=GPP methyltransferase;
DE            EC=2.1.1.255;
GN   OrderedLocusNames=SACE_3721;
OS   Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 /
OS   NBRC 13426 / NCIMB 8594 / NRRL 2338).
OC   Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC   Saccharopolyspora.
OX   NCBI_TaxID=405948;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL
RC   2338;
RX   PubMed=17369815; DOI=10.1038/nbt1297;
RA   Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S.,
RA   Haydock S.F., Leadlay P.F.;
RT   "Complete genome sequence of the erythromycin-producing bacterium
RT   Saccharopolyspora erythraea NRRL23338.";
RL   Nat. Biotechnol. 25:447-453(2007).
RN   [2]
RP   FUNCTION IN 2-METHYLISOBORNEOL BIOSYNTHESIS, AND PATHWAY.
RX   PubMed=18492804; DOI=10.1073/pnas.0802312105;
RA   Komatsu M., Tsuda M., Omura S., Oikawa H., Ikeda H.;
RT   "Identification and functional analysis of genes controlling biosynthesis
RT   of 2-methylisoborneol.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:7422-7427(2008).
CC   -!- FUNCTION: Catalyzes the SAM-dependent methylation of geranyl
CC       diphosphate (GPP) to yield (E)-2-methylgeranyl diphosphate (2-MeGPP).
CC       {ECO:0000305|PubMed:18492804}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E)-geranyl diphosphate + S-adenosyl-L-methionine = (E)-2-
CC         methylgeranyl diphosphate + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:32519, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:58057, ChEBI:CHEBI:59789, ChEBI:CHEBI:61984;
CC         EC=2.1.1.255;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305};
CC   -!- SIMILARITY: Belongs to the geranyl diphosphate 2-C-methyltransferase
CC       family. {ECO:0000305}.
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DR   EMBL; AM420293; CAM02993.1; -; Genomic_DNA.
DR   RefSeq; WP_009948569.1; NZ_PDBV01000001.1.
DR   AlphaFoldDB; A4FG18; -.
DR   SMR; A4FG18; -.
DR   STRING; 405948.SACE_3721; -.
DR   EnsemblBacteria; CAM02993; CAM02993; SACE_3721.
DR   KEGG; sen:SACE_3721; -.
DR   eggNOG; COG2230; Bacteria.
DR   HOGENOM; CLU_1057338_0_0_11; -.
DR   OMA; AHYICDI; -.
DR   OrthoDB; 1518440at2; -.
DR   BRENDA; 2.1.1.255; 5518.
DR   Proteomes; UP000006728; Chromosome.
DR   GO; GO:0008169; F:C-methyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0042214; P:terpene metabolic process; IDA:UniProtKB.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR041698; Methyltransf_25.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF13649; Methyltransf_25; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   1: Evidence at protein level;
KW   Magnesium; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..285
FT                   /note="Geranyl diphosphate 2-C-methyltransferase"
FT                   /id="PRO_0000403382"
SQ   SEQUENCE   285 AA;  31767 MW;  2A9AC15733CD2EA3 CRC64;
     MTKSIHENGT AASVYQGSIA EYWNQEANPV NLELGEVDGY FHHHYGIGEP DWSVVEGDAA
     TSHERTTREL HRLETWQAEF LLDHLGGVEP EHRIMDAGCG RGGSSFMAHE RFGCSVEGVS
     LSRKQVDFAN AQARERGVAD KVAFHQLNML DTGFDTASMR AIWNNESTMY VDLHDLFAEH
     SRLLARGGRY VTITGCYNDV YGLPSRAVST INAHYICDIH PRSGYFRAMA ANRLVPCAVV
     DLTEATVPYW RLRAKSPLAT GIEETFIEAY TSGSFQYLLI AADRV