ID   GPPMT_STRA7             Reviewed;         291 AA.
AC   A3KI18; A0A0K2AKD5;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2007, sequence version 1.
DT   03-AUG-2022, entry version 41.
DE   RecName: Full=Geranyl diphosphate 2-C-methyltransferase;
DE            Short=GPP methyltransferase;
DE            EC=2.1.1.255;
GN   ORFNames=SAM23877_0410 {ECO:0000312|EMBL:AKZ53459.1},
GN   SAML0358 {ECO:0000312|EMBL:CAJ89345.1};
OS   Streptomyces ambofaciens (strain ATCC 23877 / 3486 / DSM 40053 / JCM 4204 /
OS   NBRC 12836 / NRRL B-2516).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=278992;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23877 / 3486 / DSM 40053 / JCM 4204 / NBRC 12836 / NRRL B-2516;
RX   PubMed=16956972; DOI=10.1093/molbev/msl108;
RA   Choulet F., Aigle B., Gallois A., Mangenot S., Gerbaud C., Truong C.,
RA   Francou F.-X., Fourrier C., Guerineau M., Decaris B., Barbe V.,
RA   Pernodet J.-L., Leblond P.;
RT   "Evolution of the terminal regions of the Streptomyces linear chromosome.";
RL   Mol. Biol. Evol. 23:2361-2369(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23877 / 3486 / DSM 40053 / JCM 4204 / NBRC 12836 / NRRL B-2516;
RX   PubMed=26410452; DOI=10.1016/j.jbiotec.2015.09.020;
RA   Thibessard A., Haas D., Gerbaud C., Aigle B., Lautru S., Pernodet J.L.,
RA   Leblond P.;
RT   "Complete genome sequence of Streptomyces ambofaciens ATCC 23877, the
RT   spiramycin producer.";
RL   J. Biotechnol. 214:117-118(2015).
RN   [3]
RP   FUNCTION IN 2-METHYLISOBORNEOL BIOSYNTHESIS, AND PATHWAY.
RC   STRAIN=ATCC 23877 / 3486 / DSM 40053 / JCM 4204 / NBRC 12836 / NRRL B-2516;
RX   PubMed=18492804; DOI=10.1073/pnas.0802312105;
RA   Komatsu M., Tsuda M., Omura S., Oikawa H., Ikeda H.;
RT   "Identification and functional analysis of genes controlling biosynthesis
RT   of 2-methylisoborneol.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:7422-7427(2008).
CC   -!- FUNCTION: Catalyzes the SAM-dependent methylation of geranyl
CC       diphosphate (GPP) to yield (E)-2-methylgeranyl diphosphate (2-MeGPP).
CC       {ECO:0000305|PubMed:18492804}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E)-geranyl diphosphate + S-adenosyl-L-methionine = (E)-2-
CC         methylgeranyl diphosphate + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:32519, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:58057, ChEBI:CHEBI:59789, ChEBI:CHEBI:61984;
CC         EC=2.1.1.255;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305};
CC   -!- SIMILARITY: Belongs to the geranyl diphosphate 2-C-methyltransferase
CC       family. {ECO:0000305}.
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DR   EMBL; AM238663; CAJ89345.1; -; Genomic_DNA.
DR   EMBL; CP012382; AKZ53459.1; -; Genomic_DNA.
DR   RefSeq; WP_053126187.1; NZ_CP012382.1.
DR   AlphaFoldDB; A3KI18; -.
DR   SMR; A3KI18; -.
DR   STRING; 1889.SAM40697_0347; -.
DR   EnsemblBacteria; AKZ53459; AKZ53459; SAM23877_0410.
DR   KEGG; samb:SAM23877_0410; -.
DR   PATRIC; fig|1889.10.peg.399; -.
DR   OMA; AHFECNI; -.
DR   OrthoDB; 1518440at2; -.
DR   BRENDA; 2.1.1.255; 5973.
DR   Proteomes; UP000061018; Chromosome.
DR   GO; GO:0008169; F:C-methyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0042214; P:terpene metabolic process; IDA:UniProtKB.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR013216; Methyltransf_11.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF08241; Methyltransf_11; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   1: Evidence at protein level;
KW   Magnesium; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..291
FT                   /note="Geranyl diphosphate 2-C-methyltransferase"
FT                   /id="PRO_0000403381"
SQ   SEQUENCE   291 AA;  32488 MW;  2D6C56CE428CFA0F CRC64;
     MTTESTSTVT AKIPAPATPY QGDIARYWNN EARPVNLRLG DVDGLYHHHY GIGAVDHAAL
     GDPADSEYEK KLIAELHRLE SAQAEFLMDH LGPIGSDDTL VDAGCGRGGS MVMAHRRFGC
     KVEGVTLSAS QADFGNARAR ELRIEDHVRS RVCNMLDTPF DKGSIAASWN NESTMYVDLH
     DLFAEHSRFL EVGGRYVTIT GCWNPRYGQP SKWVSQINAH FECNIHSRRE YLRAMADNRL
     VPHTIVDLTP DTLPYWELRA TSSLVTGIEK AFIESYRDGS FQYVLIAADR V