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GPPS_MYCTU
ID   GPPS_MYCTU              Reviewed;         325 AA.
AC   O05572; I6XWR7; L0T701;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=Dimethylallyltranstransferase {ECO:0000305};
DE            EC=2.5.1.1 {ECO:0000269|PubMed:21237161, ECO:0000269|PubMed:30301210};
DE   AltName: Full=Geranyl diphosphate synthase {ECO:0000303|PubMed:21237161};
DE            Short=GPP synthase {ECO:0000303|PubMed:21237161};
GN   Name=grcC2 {ECO:0000312|EMBL:CCP43739.1};
GN   OrderedLocusNames=Rv0989c {ECO:0000312|EMBL:CCP43739.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=16586367; DOI=10.1086/502631;
RA   Jain S.K., Paul-Satyaseela M., Lamichhane G., Kim K.S., Bishai W.R.;
RT   "Mycobacterium tuberculosis invasion and traversal across an in vitro human
RT   blood-brain barrier as a pathogenic mechanism for central nervous system
RT   tuberculosis.";
RL   J. Infect. Dis. 193:1287-1295(2006).
RN   [3]
RP   FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX   PubMed=21237161; DOI=10.1016/j.febslet.2011.01.007;
RA   Mann F.M., Thomas J.A., Peters R.J.;
RT   "Rv0989c encodes a novel (E)-geranyl diphosphate synthase facilitating
RT   decaprenyl diphosphate biosynthesis in Mycobacterium tuberculosis.";
RL   FEBS Lett. 585:549-554(2011).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [5]
RP   FUNCTION, CATALYTIC ACTIVITY, DOMAIN, AND MUTAGENESIS OF ARG-92 AND
RP   ARG-217.
RX   PubMed=30301210; DOI=10.3390/molecules23102546;
RA   Nagel R., Thomas J.A., Adekunle F.A., Mann F.M., Peters R.J.;
RT   "Arginine in the FARM and SARM: a role in chain-length determination for
RT   arginine in the aspartate-rich motifs of isoprenyl diphosphate synthases
RT   from Mycobacterium tuberculosis.";
RL   Molecules 23:2546-2546(2018).
CC   -!- FUNCTION: Catalyzes the addition of isopentenyl diphosphate (IPP) onto
CC       dimethylallyl diphosphate (DMAPP) to form geranyl pyrophosphate (GPP)
CC       (PubMed:21237161, PubMed:30301210). Is probably involved in the
CC       biosynthesis of decaprenyl diphosphate, which is required for
CC       mycobacterial cell wall synthesis (PubMed:21237161). Could be required
CC       for host endothelial-cell invasion and/or intracellular survival
CC       (PubMed:16586367). {ECO:0000269|PubMed:16586367,
CC       ECO:0000269|PubMed:21237161, ECO:0000269|PubMed:30301210}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=dimethylallyl diphosphate + isopentenyl diphosphate = (2E)-
CC         geranyl diphosphate + diphosphate; Xref=Rhea:RHEA:22408,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57623, ChEBI:CHEBI:58057,
CC         ChEBI:CHEBI:128769; EC=2.5.1.1;
CC         Evidence={ECO:0000269|PubMed:21237161, ECO:0000269|PubMed:30301210};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22409;
CC         Evidence={ECO:0000269|PubMed:21237161, ECO:0000269|PubMed:30301210};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:Q12051};
CC       Note=Binds 2 Mg(2+) ions per subunit. {ECO:0000250|UniProtKB:Q12051};
CC   -!- PATHWAY: Isoprenoid biosynthesis; geranyl diphosphate biosynthesis;
CC       geranyl diphosphate from dimethylallyl diphosphate and isopentenyl
CC       diphosphate: step 1/1. {ECO:0000269|PubMed:21237161}.
CC   -!- INDUCTION: Highly up-regulated during the early stages of invasion of
CC       the human blood-brain barrier. {ECO:0000269|PubMed:16586367}.
CC   -!- DOMAIN: Contains two aspartate-rich motifs, designated as FARM (the
CC       first aspartate-rich motif) and SARM (the second aspartate-rich motif).
CC       Rv0989c contains arginine in place of the second Asp in its FARM and
CC       first Asp in its SARM. The primary role of the FARM and SARM is the
CC       chelation of the divalent magnesium ion cofactors that assist substrate
CC       binding and catalysis, but it may also play a role in determining
CC       product chain length. {ECO:0000305|PubMed:30301210}.
CC   -!- DISRUPTION PHENOTYPE: Invasion of the infant human brain microvascular
CC       endothelial-cell monolayer is significantly decreased in transposon
CC       mutant. {ECO:0000269|PubMed:16586367}.
CC   -!- SIMILARITY: Belongs to the FPP/GGPP synthase family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP43739.1; -; Genomic_DNA.
DR   RefSeq; NP_215504.1; NC_000962.3.
DR   RefSeq; WP_010886102.1; NZ_NVQJ01000018.1.
DR   AlphaFoldDB; O05572; -.
DR   SMR; O05572; -.
DR   STRING; 83332.Rv0989c; -.
DR   PaxDb; O05572; -.
DR   PRIDE; O05572; -.
DR   DNASU; 885355; -.
DR   GeneID; 885355; -.
DR   KEGG; mtu:Rv0989c; -.
DR   PATRIC; fig|83332.111.peg.1097; -.
DR   TubercuList; Rv0989c; -.
DR   eggNOG; COG0142; Bacteria.
DR   OMA; GKQMRPM; -.
DR   PhylomeDB; O05572; -.
DR   UniPathway; UPA00259; UER00368.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0004161; F:dimethylallyltranstransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004659; F:prenyltransferase activity; IBA:GO_Central.
DR   GO; GO:0033384; P:geranyl diphosphate biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0008299; P:isoprenoid biosynthetic process; IBA:GO_Central.
DR   CDD; cd00685; Trans_IPPS_HT; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR000092; Polyprenyl_synt.
DR   Pfam; PF00348; polyprenyl_synt; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   1: Evidence at protein level;
KW   Lipid biosynthesis; Lipid metabolism; Magnesium; Metal-binding;
KW   Reference proteome; Transferase.
FT   CHAIN           1..325
FT                   /note="Dimethylallyltranstransferase"
FT                   /id="PRO_0000451294"
FT   MOTIF           91..95
FT                   /note="DDXXD motif"
FT                   /evidence="ECO:0000305|PubMed:30301210"
FT   MOTIF           217..221
FT                   /note="DDXXD motif"
FT                   /evidence="ECO:0000305|PubMed:30301210"
FT   BINDING         54
FT                   /ligand="isopentenyl diphosphate"
FT                   /ligand_id="ChEBI:CHEBI:128769"
FT                   /evidence="ECO:0000250|UniProtKB:Q12051"
FT   BINDING         84
FT                   /ligand="isopentenyl diphosphate"
FT                   /ligand_id="ChEBI:CHEBI:128769"
FT                   /evidence="ECO:0000250|UniProtKB:Q12051"
FT   BINDING         91
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q12051"
FT   BINDING         91
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q12051"
FT   BINDING         95
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q12051"
FT   BINDING         95
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q12051"
FT   BINDING         101
FT                   /ligand="isopentenyl diphosphate"
FT                   /ligand_id="ChEBI:CHEBI:128769"
FT                   /evidence="ECO:0000250|UniProtKB:Q12051"
FT   SITE            92
FT                   /note="Important for determining product chain length"
FT                   /evidence="ECO:0000305|PubMed:30301210"
FT   SITE            217
FT                   /note="Important for determining product chain length"
FT                   /evidence="ECO:0000305|PubMed:30301210"
FT   MUTAGEN         92
FT                   /note="R->D: Leads to the production of longer chain
FT                   product, specifically (E,E)-farnesyl diphosphate (FPP), in
FT                   addition to GPP."
FT                   /evidence="ECO:0000269|PubMed:30301210"
FT   MUTAGEN         217
FT                   /note="R->D: Leads to the production of longer chain
FT                   product, specifically (E,E)-farnesyl diphosphate (FPP), in
FT                   addition to GPP."
FT                   /evidence="ECO:0000269|PubMed:30301210"
SQ   SEQUENCE   325 AA;  34757 MW;  95781FA1266BF960 CRC64;
     MIPAVSLGDP QFTANVHDGI ARITELINSE LSQADEVMRD TVAHLVDAGG TPFRPLFTVL
     AAQLGSDPDG WEVTVAGAAI ELMHLGTLCH DRVVDESDMS RKTPSDNTRW TNNFAILAGD
     YRFATASQLA SRLDPEAFAV VAEAFAELIT GQMRATRGPA SHIDTIEHYL RVVHEKTGSL
     IAASGQLGAA LSGAAEEQIR RVARLGRMIG AAFEISRDII AISGDSATLS GADLGQAVHT
     LPMLYALREQ TPDTSRLREL LAGPIHDDHV AEALTLLRCS PGIGKAKNVV AAYAAQAREE
     LPYLPDRQPR RALATLIDHA ISACD
 
 
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