GPR18_BOVIN
ID GPR18_BOVIN Reviewed; 332 AA.
AC Q3T0E9;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=N-arachidonyl glycine receptor;
DE Short=NAGly receptor;
DE AltName: Full=G-protein coupled receptor 18;
GN Name=GPR18;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Receptor for endocannabinoid N-arachidonyl glycine (NAGly).
CC However, conflicting results about the role of NAGly as an agonist are
CC reported. Can also be activated by plant-derived and synthetic
CC cannabinoid agonists. The activity of this receptor is mediated by G
CC proteins which inhibit adenylyl cyclase. May contribute to regulation
CC of the immune system. Is required for normal homeostasis of CD8+
CC subsets of intraepithelial lymphocytes (IELs) (CD8alphaalpha and
CC CD8alphabeta IELs) in small intstine by supporting preferential
CC migration of CD8 alphaalpha T-cells to intraepithelial compartment over
CC lamina propria compartment, and by mediating their reconstitution into
CC small intestine after bone marrow transplant. Plays a role in
CC hypotensive responses, mediating reduction in intraocular and blood
CC pressure. Mediates NAGly-induced process of reorganization of actin
CC filaments and induction of acrosomal exocytosis.
CC {ECO:0000250|UniProtKB:Q14330, ECO:0000250|UniProtKB:Q8K1Z6}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q14330};
CC Multi-pass membrane protein {ECO:0000255}. Cytoplasmic vesicle membrane
CC {ECO:0000250|UniProtKB:Q14330}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; BC102421; AAI02422.1; -; mRNA.
DR RefSeq; NP_001029861.1; NM_001034689.2.
DR AlphaFoldDB; Q3T0E9; -.
DR SMR; Q3T0E9; -.
DR STRING; 9913.ENSBTAP00000007469; -.
DR PaxDb; Q3T0E9; -.
DR PRIDE; Q3T0E9; -.
DR Ensembl; ENSBTAT00000007469; ENSBTAP00000007469; ENSBTAG00000002240.
DR GeneID; 540038; -.
DR KEGG; bta:540038; -.
DR CTD; 2841; -.
DR VEuPathDB; HostDB:ENSBTAG00000002240; -.
DR VGNC; VGNC:29574; GPR18.
DR eggNOG; ENOG502QT1V; Eukaryota.
DR GeneTree; ENSGT01050000244980; -.
DR HOGENOM; CLU_009579_8_2_1; -.
DR InParanoid; Q3T0E9; -.
DR OMA; CYLVIIY; -.
DR OrthoDB; 1122184at2759; -.
DR TreeFam; TF330775; -.
DR Reactome; R-BTA-373076; Class A/1 (Rhodopsin-like receptors).
DR Reactome; R-BTA-418594; G alpha (i) signalling events.
DR Proteomes; UP000009136; Chromosome 12.
DR Bgee; ENSBTAG00000002240; Expressed in semen and 80 other tissues.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0002300; P:CD8-positive, alpha-beta intraepithelial T cell differentiation; IEA:Ensembl.
DR GO; GO:0002305; P:CD8-positive, gamma-delta intraepithelial T cell differentiation; IEA:Ensembl.
DR GO; GO:0002689; P:negative regulation of leukocyte chemotaxis; IEA:Ensembl.
DR GO; GO:0032720; P:negative regulation of tumor necrosis factor production; IEA:Ensembl.
DR GO; GO:0051482; P:positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway; IBA:GO_Central.
DR GO; GO:0035025; P:positive regulation of Rho protein signal transduction; IBA:GO_Central.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR028335; GPR18.
DR PANTHER; PTHR24232:SF1; PTHR24232:SF1; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cytoplasmic vesicle; Disulfide bond;
KW G-protein coupled receptor; Glycoprotein; Membrane; Phosphoprotein;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..332
FT /note="N-arachidonyl glycine receptor"
FT /id="PRO_0000245013"
FT TOPO_DOM 1..26
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 27..47
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 48..56
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..77
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 78..95
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 117..138
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 160..192
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 214..233
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 255..269
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 291..332
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 323
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8K1Z6"
FT CARBOHYD 189
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 94..173
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 332 AA; 37949 MW; C536851A24AE803B CRC64;
MTTPHSQAQP GLPIDPHPDE YKVAALVFYS CIFIIGLFVN VTALWVFSCT TKKRTTVTVY
MMNVALLDLV FIMSLPFRML YYAKGEWPFG EYFCRILGAL TVFYPSIALW LLAFISADRY
MAIVQPKYAK ELKNTCKAVM ACVGVWIMTL TTTIPLLLLY EDPDTASSTP PTCLKISDII
YLKAINALNF TRLIFFFLIP LFIMIGCYLV IIHSLLHGKT SKLKPKVKEK SIRIIITLMV
QVLVCFMPFH ICFAFLMLGG DENSYNPWGA FTTFLMNLST CLDVILYYIV SKQFQARVIS
VMLYRNYLRS VRRKSFRSGS LRSLSNINSE ML
