GPR20_HUMAN
ID GPR20_HUMAN Reviewed; 358 AA.
AC Q99678; Q17R96;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=G-protein coupled receptor 20;
GN Name=GPR20;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9073069; DOI=10.1016/s0378-1119(96)00722-6;
RA O'Dowd B.F., Nguyen T., Jung B.P., Marchese A., Cheng R., Heng H.H.Q.,
RA Kolakowski L.F. Jr., Lynch K.R., George S.R.;
RT "Cloning and chromosomal mapping of four putative novel human G-protein-
RT coupled receptor genes.";
RL Gene 187:75-81(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT ARG-230.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ARG-230.
RC TISSUE=Lung, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND MUTAGENESIS OF
RP ARG-148.
RX PubMed=18347022; DOI=10.1074/jbc.m709487200;
RA Hase M., Yokomizo T., Shimizu T., Nakamura M.;
RT "Characterization of an orphan G protein-coupled receptor, GPR20, that
RT constitutively activates Gi proteins.";
RL J. Biol. Chem. 283:12747-12755(2008).
CC -!- FUNCTION: Orphan receptor with constitutive G(i) signaling activity
CC that activate cyclic AMP. {ECO:0000269|PubMed:18347022}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18347022};
CC Multi-pass membrane protein {ECO:0000269|PubMed:18347022}.
CC -!- TISSUE SPECIFICITY: Ubiquitous with highest levels in intestinal
CC tissues. In the brain detected in thalamus, putamen, and caudate, but
CC not in frontal cortex, pons and hypothalamus.
CC {ECO:0000269|PubMed:18347022}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; U66579; AAC51302.1; -; Genomic_DNA.
DR EMBL; CH471060; EAW92227.1; -; Genomic_DNA.
DR EMBL; BC117409; AAI17410.1; -; mRNA.
DR CCDS; CCDS34949.1; -.
DR RefSeq; NP_005284.2; NM_005293.2.
DR AlphaFoldDB; Q99678; -.
DR SMR; Q99678; -.
DR BioGRID; 109102; 3.
DR IntAct; Q99678; 3.
DR STRING; 9606.ENSP00000366970; -.
DR ChEMBL; CHEMBL4523912; -.
DR GlyGen; Q99678; 2 sites.
DR iPTMnet; Q99678; -.
DR PhosphoSitePlus; Q99678; -.
DR BioMuta; GPR20; -.
DR DMDM; 281185467; -.
DR CPTAC; CPTAC-1184; -.
DR PaxDb; Q99678; -.
DR PeptideAtlas; Q99678; -.
DR PRIDE; Q99678; -.
DR Antibodypedia; 14510; 259 antibodies from 29 providers.
DR DNASU; 2843; -.
DR Ensembl; ENST00000377741.4; ENSP00000366970.3; ENSG00000204882.4.
DR Ensembl; ENST00000613887.2; ENSP00000479665.1; ENSG00000275181.2.
DR GeneID; 2843; -.
DR KEGG; hsa:2843; -.
DR MANE-Select; ENST00000377741.4; ENSP00000366970.3; NM_005293.3; NP_005284.2.
DR UCSC; uc003ywf.5; human.
DR CTD; 2843; -.
DR DisGeNET; 2843; -.
DR GeneCards; GPR20; -.
DR HGNC; HGNC:4475; GPR20.
DR HPA; ENSG00000204882; Low tissue specificity.
DR MIM; 601908; gene.
DR neXtProt; NX_Q99678; -.
DR OpenTargets; ENSG00000204882; -.
DR PharmGKB; PA28863; -.
DR VEuPathDB; HostDB:ENSG00000204882; -.
DR eggNOG; ENOG502QQUE; Eukaryota.
DR GeneTree; ENSGT01040000240444; -.
DR HOGENOM; CLU_009579_8_2_1; -.
DR InParanoid; Q99678; -.
DR OMA; ISINPDM; -.
DR OrthoDB; 845400at2759; -.
DR PhylomeDB; Q99678; -.
DR TreeFam; TF330828; -.
DR PathwayCommons; Q99678; -.
DR Reactome; R-HSA-418555; G alpha (s) signalling events.
DR Reactome; R-HSA-9660821; ADORA2B mediated anti-inflammatory cytokines production.
DR SignaLink; Q99678; -.
DR BioGRID-ORCS; 2843; 23 hits in 1062 CRISPR screens.
DR GeneWiki; GPR20; -.
DR GenomeRNAi; 2843; -.
DR Pharos; Q99678; Tdark.
DR PRO; PR:Q99678; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q99678; protein.
DR Bgee; ENSG00000204882; Expressed in right coronary artery and 83 other tissues.
DR Genevisible; Q99678; HS.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR GO; GO:0043235; C:receptor complex; IDA:MGI.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IDA:UniProtKB.
DR GO; GO:0051482; P:positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway; IBA:GO_Central.
DR GO; GO:0035025; P:positive regulation of Rho protein signal transduction; IBA:GO_Central.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; G-protein coupled receptor; Glycoprotein; Membrane;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..358
FT /note="G-protein coupled receptor 20"
FT /id="PRO_0000069541"
FT TOPO_DOM 1..48
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 70..86
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..125
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 147..168
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 190..196
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 218..238
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 239..259
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 260..275
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..296
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 297..358
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 315..339
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 16
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 26
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 230
FT /note="H -> R (in dbSNP:rs10875472)"
FT /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.2"
FT /id="VAR_055917"
FT VARIANT 260
FT /note="R -> C (in dbSNP:rs36092215)"
FT /id="VAR_055918"
FT VARIANT 313
FT /note="G -> S (in dbSNP:rs34591516)"
FT /id="VAR_055919"
FT MUTAGEN 148
FT /note="R->A: Abolishes G(i) activation."
FT /evidence="ECO:0000269|PubMed:18347022"
FT CONFLICT 53..54
FT /note="WL -> CV (in Ref. 1; AAC51302)"
FT /evidence="ECO:0000305"
FT CONFLICT 157..160
FT /note="GSRR -> APAA (in Ref. 1; AAC51302)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 358 AA; 38650 MW; 5A34126C51BA732C CRC64;
MPSVSPAGPS AGAVPNATAV TTVRTNASGL EVPLFHLFAR LDEELHGTFP GLWLALMAVH
GAIFLAGLVL NGLALYVFCC RTRAKTPSVI YTINLVVTDL LVGLSLPTRF AVYYGARGCL
RCAFPHVLGY FLNMHCSILF LTCICVDRYL AIVRPEGSRR CRQPACARAV CAFVWLAAGA
VTLSVLGVTG SRPCCRVFAL TVLEFLLPLL VISVFTGRIM CALSRPGLLH QGRQRRVRAM
QLLLTVLIIF LVCFTPFHAR QVAVALWPDM PHHTSLVVYH VAVTLSSLNS CMDPIVYCFV
TSGFQATVRG LFGQHGEREP SSGDVVSMHR SSKGSGRHHI LSAGPHALTQ ALANGPEA
