GPR4_PIG
ID GPR4_PIG Reviewed; 363 AA.
AC P50132; A0A287AXR9;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 17-JUN-2020, sequence version 2.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=G-protein coupled receptor 4;
GN Name=GPR4;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8595909; DOI=10.1006/geno.1995.0013;
RA Mahadevan M.S., Baird S., Bailly J.E., Shutler G.G., Sabourin L.A.,
RA Tsilfidis C., Neville C.E., Narang M., Korneluk R.G.;
RT "Isolation of a novel G protein-coupled receptor (GPR4) localized to
RT chromosome 19q13.3.";
RL Genomics 30:84-88(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Duroc;
RG Porcine genome sequencing project;
RL Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Proton-sensing G-protein coupled receptor couples to multiple
CC intracellular signaling pathways, including GNAS/cAMP,
CC GNAQ/phospholipase C (PLC), and GNA13/Rho pathways. Acidosis-induced
CC GPR4 activation increases paracellular gap formation and permeability
CC of vascular endothelial cells through the GNA12/GNA13/Rho GTPase
CC signaling pathway. In the brain may mediate central respiratory
CC sensitivity to CO(2)/H(+). {ECO:0000250|UniProtKB:Q8BUD0}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P46093};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; U22108; AAA98458.1; -; Genomic_DNA.
DR EMBL; AEMK02000041; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; B57641; B57641.
DR RefSeq; NP_001116590.1; NM_001123118.1.
DR AlphaFoldDB; P50132; -.
DR SMR; P50132; -.
DR STRING; 9823.ENSSSCP00000027843; -.
DR PRIDE; P50132; -.
DR Ensembl; ENSSSCT00000048531; ENSSSCP00000048942; ENSSSCG00000037184.
DR Ensembl; ENSSSCT00015013399; ENSSSCP00015005178; ENSSSCG00015010239.
DR Ensembl; ENSSSCT00025068871; ENSSSCP00025029654; ENSSSCG00025050472.
DR Ensembl; ENSSSCT00030079836; ENSSSCP00030036556; ENSSSCG00030057267.
DR Ensembl; ENSSSCT00035088253; ENSSSCP00035036871; ENSSSCG00035065529.
DR Ensembl; ENSSSCT00040094910; ENSSSCP00040041994; ENSSSCG00040069335.
DR Ensembl; ENSSSCT00045017735; ENSSSCP00045012208; ENSSSCG00045010480.
DR Ensembl; ENSSSCT00055018646; ENSSSCP00055014687; ENSSSCG00055009564.
DR Ensembl; ENSSSCT00060026499; ENSSSCP00060011277; ENSSSCG00060019629.
DR Ensembl; ENSSSCT00065004228; ENSSSCP00065001719; ENSSSCG00065003170.
DR GeneID; 100144489; -.
DR KEGG; ssc:100144489; -.
DR CTD; 2828; -.
DR VGNC; VGNC:88631; GPR4.
DR eggNOG; ENOG502QS9G; Eukaryota.
DR GeneTree; ENSGT01050000244810; -.
DR InParanoid; P50132; -.
DR OMA; RTWEGCH; -.
DR OrthoDB; 716326at2759; -.
DR Proteomes; UP000008227; Chromosome 6.
DR Proteomes; UP000314985; Unplaced.
DR Bgee; ENSSSCG00000037184; Expressed in oocyte and 24 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; ISS:UniProtKB.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0050729; P:positive regulation of inflammatory response; ISS:UniProtKB.
DR GO; GO:0030155; P:regulation of cell adhesion; ISS:UniProtKB.
DR GO; GO:0043114; P:regulation of vascular permeability; ISS:UniProtKB.
DR GO; GO:0010447; P:response to acidic pH; ISS:UniProtKB.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR002276; GPR4_orph.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01147; GPR4RECEPTOR.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 3: Inferred from homology;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..363
FT /note="G-protein coupled receptor 4"
FT /id="PRO_0000069513"
FT TOPO_DOM 1..17
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 18..42
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 43..54
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..76
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..91
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..113
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 114..132
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..154
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..179
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..201
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 202..224
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 246..263
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 285..363
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 344..363
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 3
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 164
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 90..168
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 38
FT /note="A -> R (in Ref. 1; AAA98458)"
FT /evidence="ECO:0000305"
FT CONFLICT 341..350
FT /note="GWVASPPSQG -> AGWHLRPPR (in Ref. 1; AAA98458)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 363 AA; 40974 MW; 04610250913FF42D CRC64;
MGNGTWEGCH VDSRVDHLFP PSLYIFVIGV GLPTNCLALW AAYRQVRQRN ELGVYLMNLS
IADLLYICTL PLWVDYFLHH DNWIHGPGSC KLFGFIFYTN IYISIAFLCC ISVDRYLAVA
HPLRFARLRR VKTAVAVSSV VWATELGANS VPLFHDELFR DRYNHTFCFE KFPMEGWVAW
MNLYRVFVGF LFPWALMLLS YRGILRAVRG SVSTERQEKA KIKRLALSLI AIVLVCFAPY
HVLLLSRSAV YLGHPWDCGF EERVFSAYHS SLAFTSLNCV ADPILYCLVN EGARSDVAKA
LHNLLRFLTS DKPQEMASAS LTLDTPLTSK RNSMARAVAA GWVASPPSQG DQVQLKMLPP
PAP
