GPR4_RAT
ID GPR4_RAT Reviewed; 365 AA.
AC Q4KLH9;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 02-AUG-2005, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=G-protein coupled receptor 4;
GN Name=Gpr4;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Proton-sensing G-protein coupled receptor couples to multiple
CC intracellular signaling pathways, including GNAS/cAMP,
CC GNAQ/phospholipase C (PLC), and GNA13/Rho pathways. Acidosis-induced
CC GPR4 activation increases paracellular gap formation and permeability
CC of vascular endothelial cells through the GNA12/GNA13/Rho GTPase
CC signaling pathway. In the brain may mediate central respiratory
CC sensitivity to CO(2)/H(+). {ECO:0000250|UniProtKB:Q8BUD0}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P46093};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; BC099196; AAH99196.1; -; mRNA.
DR RefSeq; NP_001020851.1; NM_001025680.1.
DR RefSeq; XP_006228480.1; XM_006228418.1.
DR RefSeq; XP_006228481.1; XM_006228419.1.
DR AlphaFoldDB; Q4KLH9; -.
DR SMR; Q4KLH9; -.
DR STRING; 10116.ENSRNOP00000021894; -.
DR BindingDB; Q4KLH9; -.
DR ChEMBL; CHEMBL4105950; -.
DR GuidetoPHARMACOLOGY; 84; -.
DR GlyGen; Q4KLH9; 2 sites.
DR PhosphoSitePlus; Q4KLH9; -.
DR PaxDb; Q4KLH9; -.
DR Ensembl; ENSRNOT00000021893; ENSRNOP00000021894; ENSRNOG00000016362.
DR GeneID; 308408; -.
DR KEGG; rno:308408; -.
DR UCSC; RGD:1311604; rat.
DR CTD; 2828; -.
DR RGD; 1311604; Gpr4.
DR eggNOG; ENOG502QS9G; Eukaryota.
DR GeneTree; ENSGT01050000244810; -.
DR HOGENOM; CLU_009579_8_2_1; -.
DR InParanoid; Q4KLH9; -.
DR OMA; RTWEGCH; -.
DR OrthoDB; 716326at2759; -.
DR PhylomeDB; Q4KLH9; -.
DR TreeFam; TF331803; -.
DR Reactome; R-RNO-373076; Class A/1 (Rhodopsin-like receptors).
DR Reactome; R-RNO-416476; G alpha (q) signalling events.
DR PRO; PR:Q4KLH9; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000016362; Expressed in heart and 18 other tissues.
DR Genevisible; Q4KLH9; RN.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; ISS:UniProtKB.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0060055; P:angiogenesis involved in wound healing; ISO:RGD.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISO:RGD.
DR GO; GO:0072144; P:glomerular mesangial cell development; ISO:RGD.
DR GO; GO:0016525; P:negative regulation of angiogenesis; ISO:RGD.
DR GO; GO:0007200; P:phospholipase C-activating G protein-coupled receptor signaling pathway; ISO:RGD.
DR GO; GO:0051482; P:positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway; IBA:GO_Central.
DR GO; GO:0050729; P:positive regulation of inflammatory response; ISS:UniProtKB.
DR GO; GO:0035025; P:positive regulation of Rho protein signal transduction; ISO:RGD.
DR GO; GO:0030155; P:regulation of cell adhesion; ISS:UniProtKB.
DR GO; GO:0043114; P:regulation of vascular permeability; ISS:UniProtKB.
DR GO; GO:0010447; P:response to acidic pH; ISS:UniProtKB.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR002276; GPR4_orph.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01147; GPR4RECEPTOR.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..365
FT /note="G-protein coupled receptor 4"
FT /id="PRO_0000379520"
FT TOPO_DOM 1..24
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 25..45
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 46..55
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..94
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 116..135
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 157..179
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..226
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 248..273
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..291
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 292..365
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 3
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 166
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 92..170
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 365 AA; 41267 MW; C198D70B2117559B CRC64;
MDNSTGTWEG CHVDSRVDHL FPPSLYIFVI GVGLPTNCLA LWAAYRQVRQ RNELGVYLMN
LSIADLLYIC TLPLWVDYFL HHDNWIHGPG SCKLFGFIFY SNIYISIAFL CCISVDRYLA
VAHPLRFARL RRVKTAVAVS SVVWATELGA NSAPLFHDEL FRDRYNHTFC FEKFPMERWV
AWMNLYRVFV GFLFPWALML LCYRGILRAV QSSVSTERQE KVKIKRLALS LIAIVLVCFA
PYHALLLSRS AVYLGRPWDC GFEERVFSAY HSSLAFTSLN CVADPILYCL VNEGARSDVA
KALHNLLRFL ASNKPQEMAN ASLTLETPLT SKRSTTGKTS GAVWAVPPTA QGDQVPLKVL
LPPAQ
