GPR78_HUMAN
ID GPR78_HUMAN Reviewed; 363 AA.
AC Q96P69; Q8NGV3;
DT 03-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=G-protein coupled receptor 78;
GN Name=GPR78; ORFNames=UNQ5925/PRO19818;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11574155; DOI=10.1016/s0378-1119(01)00651-5;
RA Lee D.K., Nguyen T., Lynch K.R., Cheng R., Vanti W.B., Arkhitko O.,
RA Lewis T., Evans J.F., George S.R., O'Dowd B.F.;
RT "Discovery and mapping of ten novel G protein-coupled receptor genes.";
RL Gene 275:83-91(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S.,
RA Tsutsumi S., Aburatani H., Asai K., Akiyama Y.;
RT "Genome-wide discovery and analysis of human seven transmembrane helix
RT receptor genes.";
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND MUTAGENESIS OF ARG-236.
RX PubMed=17363172; DOI=10.1016/j.bbagen.2007.01.013;
RA Jones P.G., Nawoschik S.P., Sreekumar K., Uveges A.J., Tseng E., Zhang L.,
RA Johnson J., He L., Paulsen J.E., Bates B., Pausch M.H.;
RT "Tissue distribution and functional analyses of the constitutively active
RT orphan G protein coupled receptors, GPR26 and GPR78.";
RL Biochim. Biophys. Acta 1770:890-901(2007).
CC -!- FUNCTION: Orphan receptor. Displays a significant level of constitutive
CC activity. Its effect is mediated by G(s)-alpha protein that stimulate
CC adenylate cyclase, resulting in an elevation of intracellular cAMP.
CC {ECO:0000269|PubMed:17363172}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: High level of expression in placenta. Expressed
CC throughout the brain at low level. No expression detected in skeletal
CC muscle, lung, heart, liver, pancreas, or kidney.
CC {ECO:0000269|PubMed:17363172}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AF411107; AAL26479.1; -; mRNA.
DR EMBL; AB065673; BAC05898.1; -; Genomic_DNA.
DR EMBL; AY359107; AAQ89465.1; -; mRNA.
DR EMBL; BC057778; AAH57778.1; -; mRNA.
DR EMBL; BC069343; AAH69343.1; -; mRNA.
DR EMBL; BC069813; AAH69813.1; -; mRNA.
DR CCDS; CCDS3403.1; -.
DR RefSeq; NP_543009.2; NM_080819.4.
DR AlphaFoldDB; Q96P69; -.
DR SMR; Q96P69; -.
DR BioGRID; 118076; 1.
DR STRING; 9606.ENSP00000371927; -.
DR ChEMBL; CHEMBL4523908; -.
DR iPTMnet; Q96P69; -.
DR PhosphoSitePlus; Q96P69; -.
DR BioMuta; GPR78; -.
DR MassIVE; Q96P69; -.
DR PaxDb; Q96P69; -.
DR PeptideAtlas; Q96P69; -.
DR PRIDE; Q96P69; -.
DR Antibodypedia; 9632; 209 antibodies from 29 providers.
DR DNASU; 27201; -.
DR Ensembl; ENST00000382487.5; ENSP00000371927.4; ENSG00000155269.12.
DR GeneID; 27201; -.
DR KEGG; hsa:27201; -.
DR MANE-Select; ENST00000382487.5; ENSP00000371927.4; NM_080819.5; NP_543009.2.
DR UCSC; uc003glk.5; human.
DR CTD; 27201; -.
DR DisGeNET; 27201; -.
DR GeneCards; GPR78; -.
DR HGNC; HGNC:4528; GPR78.
DR HPA; ENSG00000155269; Tissue enhanced (esophagus, placenta).
DR MIM; 606921; gene.
DR neXtProt; NX_Q96P69; -.
DR OpenTargets; ENSG00000155269; -.
DR PharmGKB; PA28921; -.
DR VEuPathDB; HostDB:ENSG00000155269; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00950000183001; -.
DR InParanoid; Q96P69; -.
DR OMA; MDIVTMK; -.
DR OrthoDB; 1039292at2759; -.
DR PhylomeDB; Q96P69; -.
DR TreeFam; TF332434; -.
DR PathwayCommons; Q96P69; -.
DR BioGRID-ORCS; 27201; 8 hits in 1063 CRISPR screens.
DR ChiTaRS; GPR78; human.
DR GeneWiki; GPR78; -.
DR GenomeRNAi; 27201; -.
DR Pharos; Q96P69; Tbio.
DR PRO; PR:Q96P69; -.
DR Proteomes; UP000005640; Chromosome 4.
DR RNAct; Q96P69; protein.
DR Bgee; ENSG00000155269; Expressed in lower esophagus mucosa and 59 other tissues.
DR ExpressionAtlas; Q96P69; baseline and differential.
DR Genevisible; Q96P69; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IDA:UniProtKB.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IDA:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Membrane;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..363
FT /note="G-protein coupled receptor 78"
FT /id="PRO_0000069585"
FT TOPO_DOM 1..7
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 8..28
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 29..47
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..80
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..122
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 144..168
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 190..242
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 264..277
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 278..297
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 298..363
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 340..363
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 341..363
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 79..156
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VARIANT 201
FT /note="R -> S (in dbSNP:rs17844778)"
FT /id="VAR_024258"
FT VARIANT 330
FT /note="A -> T (in dbSNP:rs11941659)"
FT /id="VAR_033474"
FT VARIANT 342
FT /note="R -> H (in dbSNP:rs9685931)"
FT /id="VAR_033475"
FT MUTAGEN 236
FT /note="R->E: No effect on constitutive activity."
FT /evidence="ECO:0000269|PubMed:17363172"
FT CONFLICT 216
FT /note="L -> V (in Ref. 1; AAL26479)"
FT /evidence="ECO:0000305"
FT CONFLICT 226..227
FT /note="QR -> HG (in Ref. 1; AAL26479)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 363 AA; 39332 MW; 2C0052A0A1A22A93 CRC64;
MGPGEALLAG LLVMVLAVAL LSNALVLLCC AYSAELRTRA SGVLLVNLSL GHLLLAALDM
PFTLLGVMRG RTPSAPGACQ VIGFLDTFLA SNAALSVAAL SADQWLAVGF PLRYAGRLRP
RYAGLLLGCA WGQSLAFSGA ALGCSWLGYS SAFASCSLRL PPEPERPRFA AFTATLHAVG
FVLPLAVLCL TSLQVHRVAR RHCQRMDTVT MKALALLADL HPSVRQRCLI QQKRRRHRAT
RKIGIAIATF LICFAPYVMT RLAELVPFVT VNAQWGILSK CLTYSKAVAD PFTYSLLRRP
FRQVLAGMVH RLLKRTPRPA STHDSSLDVA GMVHQLLKRT PRPASTHNGS VDTENDSCLQ
QTH