GPRL1_HUMAN
ID GPRL1_HUMAN Reviewed; 242 AA.
AC Q6UWM5; Q96L06;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=GLIPR1-like protein 1;
DE Flags: Precursor;
GN Name=GLIPR1L1; ORFNames=UNQ2972/PRO7434;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=16714093; DOI=10.1016/j.ygeno.2006.03.021;
RA Ren C., Ren C.-H., Li L., Goltsov A.A., Thompson T.C.;
RT "Identification and characterization of RTVP1/GLIPR1-like genes, a novel
RT p53 target gene cluster.";
RL Genomics 88:163-172(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PROTEIN SEQUENCE OF 23-37.
RX PubMed=15340161; DOI=10.1110/ps.04682504;
RA Zhang Z., Henzel W.J.;
RT "Signal peptide prediction based on analysis of experimentally verified
RT cleavage sites.";
RL Protein Sci. 13:2819-2824(2004).
CC -!- FUNCTION: Plays a role in the binding between sperm and oocytes.
CC Component of epididymosomes, one type of membranous microvesicules
CC which mediate the transfer of lipids and proteins to spermatozoa plasma
CC membrane during epididymal maturation. Also a component of the CD9-
CC positive microvesicules found in the cauda region.
CC {ECO:0000250|UniProtKB:Q32LB5, ECO:0000250|UniProtKB:Q9DAG6}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC {ECO:0000250|UniProtKB:Q9DAG6}. Cell membrane
CC {ECO:0000250|UniProtKB:Q9DAG6}; Lipid-anchor, GPI-anchor
CC {ECO:0000250|UniProtKB:Q9DAG6}. Membrane raft
CC {ECO:0000250|UniProtKB:Q32LB5}. Note=Located in the connecting piece of
CC elongated spermatids and sperm. Also located in the apical region of
CC the sperm head after sperm capacitation. Located on sperm equatorial
CC segment and neck. Associated with epididymosomes from the caput and
CC cauda epididymis. {ECO:0000250|UniProtKB:Q32LB5,
CC ECO:0000250|UniProtKB:Q9DAG6}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=GLIPR1L1beta;
CC IsoId=Q6UWM5-1; Sequence=Displayed;
CC Name=2; Synonyms=GLIPR1L1alpha;
CC IsoId=Q6UWM5-2; Sequence=VSP_022456;
CC -!- TISSUE SPECIFICITY: Highly expressed in testis.
CC {ECO:0000269|PubMed:16714093}.
CC -!- PTM: N-glycosylated. N-glycosylation decreases during the transit in
CC the caput. {ECO:0000250|UniProtKB:Q32LB5}.
CC -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
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DR EMBL; AY358731; AAQ89093.1; -; mRNA.
DR EMBL; BC014603; AAH14603.1; -; mRNA.
DR CCDS; CCDS76578.1; -. [Q6UWM5-1]
DR CCDS; CCDS9009.1; -. [Q6UWM5-2]
DR RefSeq; NP_001291893.1; NM_001304964.1. [Q6UWM5-1]
DR RefSeq; NP_689992.1; NM_152779.3. [Q6UWM5-2]
DR AlphaFoldDB; Q6UWM5; -.
DR SMR; Q6UWM5; -.
DR BioGRID; 129177; 2.
DR STRING; 9606.ENSP00000310770; -.
DR GlyGen; Q6UWM5; 1 site.
DR iPTMnet; Q6UWM5; -.
DR PhosphoSitePlus; Q6UWM5; -.
DR BioMuta; GLIPR1L1; -.
DR DMDM; 124007190; -.
DR MassIVE; Q6UWM5; -.
DR PaxDb; Q6UWM5; -.
DR PeptideAtlas; Q6UWM5; -.
DR PRIDE; Q6UWM5; -.
DR ProteomicsDB; 67498; -. [Q6UWM5-1]
DR ProteomicsDB; 67499; -. [Q6UWM5-2]
DR Antibodypedia; 29594; 168 antibodies from 25 providers.
DR DNASU; 256710; -.
DR Ensembl; ENST00000312442.2; ENSP00000310770.2; ENSG00000173401.10. [Q6UWM5-2]
DR Ensembl; ENST00000378695.9; ENSP00000367967.4; ENSG00000173401.10. [Q6UWM5-1]
DR GeneID; 256710; -.
DR KEGG; hsa:256710; -.
DR MANE-Select; ENST00000378695.9; ENSP00000367967.4; NM_001304964.2; NP_001291893.1.
DR UCSC; uc001sxn.4; human. [Q6UWM5-1]
DR CTD; 256710; -.
DR DisGeNET; 256710; -.
DR GeneCards; GLIPR1L1; -.
DR HGNC; HGNC:28392; GLIPR1L1.
DR HPA; ENSG00000173401; Tissue enriched (testis).
DR MIM; 610395; gene.
DR neXtProt; NX_Q6UWM5; -.
DR OpenTargets; ENSG00000173401; -.
DR PharmGKB; PA145008265; -.
DR VEuPathDB; HostDB:ENSG00000173401; -.
DR eggNOG; KOG3017; Eukaryota.
DR GeneTree; ENSGT00940000162547; -.
DR HOGENOM; CLU_035730_2_0_1; -.
DR InParanoid; Q6UWM5; -.
DR OMA; SIFVCNY; -.
DR OrthoDB; 1528782at2759; -.
DR PhylomeDB; Q6UWM5; -.
DR TreeFam; TF316148; -.
DR PathwayCommons; Q6UWM5; -.
DR BioGRID-ORCS; 256710; 12 hits in 1063 CRISPR screens.
DR GenomeRNAi; 256710; -.
DR Pharos; Q6UWM5; Tdark.
DR PRO; PR:Q6UWM5; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q6UWM5; protein.
DR Bgee; ENSG00000173401; Expressed in sperm and 95 other tissues.
DR Genevisible; Q6UWM5; HS.
DR GO; GO:0001669; C:acrosomal vesicle; IEA:UniProtKB-SubCell.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0045121; C:membrane raft; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0007338; P:single fertilization; IEA:UniProtKB-KW.
DR CDD; cd05385; CAP_GLIPR1-like; 1.
DR Gene3D; 3.40.33.10; -; 1.
DR InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR InterPro; IPR014044; CAP_domain.
DR InterPro; IPR035940; CAP_sf.
DR InterPro; IPR001283; CRISP-related.
DR InterPro; IPR034121; SCP_GLIPR-1-like.
DR InterPro; IPR002413; V5_allergen-like.
DR PANTHER; PTHR10334; PTHR10334; 1.
DR Pfam; PF00188; CAP; 1.
DR PRINTS; PR00838; V5ALLERGEN.
DR PRINTS; PR00837; V5TPXLIKE.
DR SMART; SM00198; SCP; 1.
DR SUPFAM; SSF55797; SSF55797; 1.
DR PROSITE; PS01009; CRISP_1; 1.
DR PROSITE; PS01010; CRISP_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Cytoplasmic vesicle;
KW Direct protein sequencing; Fertilization; Glycoprotein; GPI-anchor;
KW Lipoprotein; Membrane; Reference proteome; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000269|PubMed:15340161"
FT CHAIN 23..221
FT /note="GLIPR1-like protein 1"
FT /id="PRO_0000272653"
FT PROPEP 222..242
FT /note="Removed in mature form"
FT /evidence="ECO:0000305"
FT /id="PRO_0000441107"
FT DOMAIN 39..171
FT /note="SCP"
FT /evidence="ECO:0000255"
FT LIPID 221
FT /note="GPI-anchor amidated glycine"
FT /evidence="ECO:0000255"
FT CARBOHYD 119
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT VAR_SEQ 204..213
FT /note="RTPQLIIPNQ -> K (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16714093"
FT /id="VSP_022456"
FT CONFLICT 184
FT /note="V -> A (in Ref. 2; AAQ89093)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 242 AA; 27151 MW; 22843450EC652C76 CRC64;
MALKNKFSCL WILGLCLVAT TSSKIPSITD PHFIDNCIEA HNEWRGKVNP PAADMKYMIW
DKGLAKMAKA WANQCKFEHN DCLDKSYKCY AAFEYVGENI WLGGIKSFTP RHAITAWYNE
TQFYDFDSLS CSRVCGHYTQ LVWANSFYVG CAVAMCPNLG GASTAIFVCN YGPAGNFANM
PPYVRGESCS LCSKEEKCVK NLCRTPQLII PNQNPFLKPT GRAPQQTAFN PFSLGFLLLR
IF
