GPR_BACAN
ID GPR_BACAN Reviewed; 368 AA.
AC Q81LR5; Q6HT70; Q6KMG0;
DT 25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Germination protease {ECO:0000255|HAMAP-Rule:MF_00626};
DE EC=3.4.24.78 {ECO:0000255|HAMAP-Rule:MF_00626};
DE AltName: Full=GPR endopeptidase {ECO:0000255|HAMAP-Rule:MF_00626};
DE AltName: Full=Germination proteinase {ECO:0000255|HAMAP-Rule:MF_00626};
DE AltName: Full=Spore protease {ECO:0000255|HAMAP-Rule:MF_00626};
DE Flags: Precursor;
GN Name=gpr {ECO:0000255|HAMAP-Rule:MF_00626};
GN OrderedLocusNames=BA_4546, GBAA_4546, BAS4220;
OS Bacillus anthracis.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1392;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames / isolate Porton;
RX PubMed=12721629; DOI=10.1038/nature01586;
RA Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T.,
RA Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R.,
RA Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M.,
RA Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M.L.,
RA DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C.,
RA Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y.,
RA Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M.,
RA Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E.,
RA White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M.,
RA Hanna P.C., Kolstoe A.-B., Fraser C.M.;
RT "The genome sequence of Bacillus anthracis Ames and comparison to closely
RT related bacteria.";
RL Nature 423:81-86(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames ancestor;
RX PubMed=18952800; DOI=10.1128/jb.01347-08;
RA Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL J. Bacteriol. 191:445-446(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sterne;
RA Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA Richardson P., Rubin E., Tice H.;
RT "Complete genome sequence of Bacillus anthracis Sterne.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Initiates the rapid degradation of small, acid-soluble
CC proteins during spore germination. {ECO:0000255|HAMAP-Rule:MF_00626}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endopeptidase action with P4 Glu or Asp, P1 preferably Glu >
CC Asp, P1' hydrophobic and P2' Ala.; EC=3.4.24.78;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00626};
CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00626}.
CC -!- PTM: Autoproteolytically processed. The inactive tetrameric zymogen
CC termed p46 autoprocesses to a smaller form termed p41, which is active
CC only during spore germination. {ECO:0000255|HAMAP-Rule:MF_00626}.
CC -!- SIMILARITY: Belongs to the peptidase A25 family. {ECO:0000255|HAMAP-
CC Rule:MF_00626}.
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DR EMBL; AE016879; AAP28255.1; -; Genomic_DNA.
DR EMBL; AE017334; AAT33667.1; -; Genomic_DNA.
DR EMBL; AE017225; AAT56519.1; -; Genomic_DNA.
DR RefSeq; NP_846769.1; NC_003997.3.
DR RefSeq; WP_000662639.1; NZ_WXXJ01000027.1.
DR RefSeq; YP_030468.1; NC_005945.1.
DR AlphaFoldDB; Q81LR5; -.
DR SMR; Q81LR5; -.
DR STRING; 260799.BAS4220; -.
DR MEROPS; A25.001; -.
DR DNASU; 1088279; -.
DR EnsemblBacteria; AAP28255; AAP28255; BA_4546.
DR EnsemblBacteria; AAT33667; AAT33667; GBAA_4546.
DR GeneID; 45024198; -.
DR KEGG; ban:BA_4546; -.
DR KEGG; bar:GBAA_4546; -.
DR KEGG; bat:BAS4220; -.
DR PATRIC; fig|198094.11.peg.4514; -.
DR eggNOG; COG0680; Bacteria.
DR HOGENOM; CLU_055087_1_0_9; -.
DR OMA; PMGNYIT; -.
DR Proteomes; UP000000427; Chromosome.
DR Proteomes; UP000000594; Chromosome.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-UniRule.
DR GO; GO:0009847; P:spore germination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.1450; -; 1.
DR HAMAP; MF_00626; Germination_prot; 1.
DR InterPro; IPR023430; Pept_HybD-like_dom_sf.
DR InterPro; IPR005080; Peptidase_A25.
DR Pfam; PF03418; Peptidase_A25; 1.
DR PIRSF; PIRSF019549; Peptidase_A25; 1.
DR SUPFAM; SSF53163; SSF53163; 1.
DR TIGRFAMs; TIGR01441; GPR; 1.
PE 3: Inferred from homology;
KW Hydrolase; Protease; Reference proteome; Zymogen.
FT PROPEP 1..15
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00626"
FT /id="PRO_0000026856"
FT CHAIN 16..368
FT /note="Germination protease"
FT /id="PRO_0000026857"
SQ SEQUENCE 368 AA; 40605 MW; 7FA028D9D5D24865 CRC64;
MKEPLDLSKY SVRTDLAVEA HQMLQERQEE QQQGIQGVIV KEREEEGIII TKVTIDEVAS
ESMGKKPGNY LTLEVQGIRQ QDTELQQKVE RIFAKEFSYF LEEVGVTKEA SCLIVGLGNW
NVTPDALGPI VVENVLVTRH LFQLQPESVE EGFRPVSAIR PGVMGITGIE TSDVIYGIIE
KTKPDFVIAI DALAARSIER VNSTIQISDT GIHPGSGVGN KRKELSKETL GIPVIAIGVP
TVVDAVSITS DTIDFILKHF GREMKEGNKP SRSLLPAGFT FGEKKKLTEE DMPDEKSRNM
FLGAVGTLED EEKRKLIYEV LSPLGHNLMV TPKEVDAFIE DMANVIASGL NAALHHQIDQ
DNTGAYTH