GPR_CALS4
ID GPR_CALS4 Reviewed; 324 AA.
AC Q8RB77;
DT 25-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Germination protease {ECO:0000255|HAMAP-Rule:MF_00626};
DE EC=3.4.24.78 {ECO:0000255|HAMAP-Rule:MF_00626};
DE AltName: Full=GPR endopeptidase {ECO:0000255|HAMAP-Rule:MF_00626};
DE AltName: Full=Germination proteinase {ECO:0000255|HAMAP-Rule:MF_00626};
DE AltName: Full=Spore protease {ECO:0000255|HAMAP-Rule:MF_00626};
DE Flags: Precursor;
GN Name=gpr {ECO:0000255|HAMAP-Rule:MF_00626}; OrderedLocusNames=TTE0946;
OS Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM
OS 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis).
OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Caldanaerobacter.
OX NCBI_TaxID=273068;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15242 / JCM 11007 / NBRC 100824 / MB4;
RX PubMed=11997336; DOI=10.1101/gr.219302;
RA Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y.,
RA Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R.,
RA Wang J., Yu J., Yang H.;
RT "A complete sequence of the T. tengcongensis genome.";
RL Genome Res. 12:689-700(2002).
CC -!- FUNCTION: Initiates the rapid degradation of small, acid-soluble
CC proteins during spore germination. {ECO:0000255|HAMAP-Rule:MF_00626}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endopeptidase action with P4 Glu or Asp, P1 preferably Glu >
CC Asp, P1' hydrophobic and P2' Ala.; EC=3.4.24.78;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00626};
CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00626}.
CC -!- PTM: Autoproteolytically processed. The inactive tetrameric zymogen
CC termed p46 autoprocesses to a smaller form termed p41, which is active
CC only during spore germination. {ECO:0000255|HAMAP-Rule:MF_00626}.
CC -!- SIMILARITY: Belongs to the peptidase A25 family. {ECO:0000255|HAMAP-
CC Rule:MF_00626}.
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DR EMBL; AE008691; AAM24202.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8RB77; -.
DR SMR; Q8RB77; -.
DR STRING; 273068.TTE0946; -.
DR MEROPS; A25.001; -.
DR EnsemblBacteria; AAM24202; AAM24202; TTE0946.
DR KEGG; tte:TTE0946; -.
DR eggNOG; COG0680; Bacteria.
DR HOGENOM; CLU_055087_1_0_9; -.
DR OMA; PMGNYIT; -.
DR Proteomes; UP000000555; Chromosome.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-UniRule.
DR GO; GO:0009847; P:spore germination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.1450; -; 1.
DR HAMAP; MF_00626; Germination_prot; 1.
DR InterPro; IPR023430; Pept_HybD-like_dom_sf.
DR InterPro; IPR005080; Peptidase_A25.
DR Pfam; PF03418; Peptidase_A25; 1.
DR PIRSF; PIRSF019549; Peptidase_A25; 1.
DR SUPFAM; SSF53163; SSF53163; 1.
DR TIGRFAMs; TIGR01441; GPR; 1.
PE 3: Inferred from homology;
KW Hydrolase; Protease; Reference proteome; Zymogen.
FT PROPEP 1..10
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00626"
FT /id="PRO_0000026880"
FT CHAIN 11..324
FT /note="Germination protease"
FT /id="PRO_0000026881"
SQ SEQUENCE 324 AA; 35519 MW; D4E8B6C5763C3D7C CRC64;
MIIVLGIRTD LALEARELYK EREIPGVSIR EEGEEGIKIT RVKILDERGE KAMGKPVGDY
ITIEAPGLLE RDLDLEERVA KVLANIISEL AQLKKDSHVL VVGLGNWNVT PDALGPRVVS
NIVVTRHLKE YAPLQFGDEI RSVSAFSPGV LGITGIETAE ILKGVVDRVK PDLVITIDAL
ASRRLERLST TIQISNTGIS PGSGVGNRRL SITSESLGVP VIAIGVPTVV DAVTIAHDTI
EYLVKELSEQ TSKESVFYKV LENMNKQEKY SLIEEVLSPY VQNLVVTPKE IDLLIKNIAL
VISRGINLAL QPGLTEKEMN QLLH
