GPSM3_MOUSE
ID GPSM3_MOUSE Reviewed; 159 AA.
AC Q3U1Z5; Q3U1M8; Q3UJZ5; Q8VDF9;
DT 02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2006, sequence version 2.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=G-protein-signaling modulator 3;
GN Name=Gpsm3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Czech II, and FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38; SER-55 AND SER-58, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Interacts with subunit of G(i) alpha proteins and regulates
CC the activation of G(i) alpha proteins. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3U1Z5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3U1Z5-2; Sequence=VSP_018153, VSP_018154;
CC -!- DOMAIN: The GoLoco 1 and/or GoLoco 3 domains exhibit GDI activity
CC towards GDP-bound G(i) alpha protein, but not the GoLoco 2 domain.
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE27009.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK146245; BAE27009.1; ALT_FRAME; mRNA.
DR EMBL; AK155617; BAE33348.1; -; mRNA.
DR EMBL; AK155863; BAE33467.1; -; mRNA.
DR EMBL; BC021942; AAH21942.1; -; mRNA.
DR EMBL; BC080804; AAH80804.1; -; mRNA.
DR CCDS; CCDS37589.1; -. [Q3U1Z5-1]
DR RefSeq; NP_598877.1; NM_134116.5. [Q3U1Z5-1]
DR AlphaFoldDB; Q3U1Z5; -.
DR STRING; 10090.ENSMUSP00000045911; -.
DR iPTMnet; Q3U1Z5; -.
DR PhosphoSitePlus; Q3U1Z5; -.
DR EPD; Q3U1Z5; -.
DR jPOST; Q3U1Z5; -.
DR MaxQB; Q3U1Z5; -.
DR PaxDb; Q3U1Z5; -.
DR PRIDE; Q3U1Z5; -.
DR ProteomicsDB; 271049; -. [Q3U1Z5-1]
DR ProteomicsDB; 271050; -. [Q3U1Z5-2]
DR Antibodypedia; 28524; 66 antibodies from 18 providers.
DR DNASU; 106512; -.
DR Ensembl; ENSMUST00000038244; ENSMUSP00000045911; ENSMUSG00000034786. [Q3U1Z5-1]
DR GeneID; 106512; -.
DR KEGG; mmu:106512; -.
DR UCSC; uc012aqd.1; mouse. [Q3U1Z5-1]
DR CTD; 63940; -.
DR MGI; MGI:2146785; Gpsm3.
DR VEuPathDB; HostDB:ENSMUSG00000034786; -.
DR eggNOG; ENOG502STIS; Eukaryota.
DR GeneTree; ENSGT00390000002471; -.
DR HOGENOM; CLU_139851_0_0_1; -.
DR InParanoid; Q3U1Z5; -.
DR OMA; HQSQRME; -.
DR PhylomeDB; Q3U1Z5; -.
DR TreeFam; TF339136; -.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR BioGRID-ORCS; 106512; 0 hits in 71 CRISPR screens.
DR ChiTaRS; Gpsm3; mouse.
DR PRO; PR:Q3U1Z5; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q3U1Z5; protein.
DR Bgee; ENSMUSG00000034786; Expressed in granulocyte and 77 other tissues.
DR ExpressionAtlas; Q3U1Z5; baseline and differential.
DR Genevisible; Q3U1Z5; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0030695; F:GTPase regulator activity; IEA:InterPro.
DR GO; GO:1900017; P:positive regulation of cytokine production involved in inflammatory response; IMP:MGI.
DR GO; GO:0050729; P:positive regulation of inflammatory response; IMP:MGI.
DR GO; GO:0002690; P:positive regulation of leukocyte chemotaxis; IMP:MGI.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR003109; GoLoco_motif.
DR InterPro; IPR042888; GPSM3.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR47617; PTHR47617; 1.
DR Pfam; PF02188; GoLoco; 2.
DR SMART; SM00390; GoLoco; 3.
DR PROSITE; PS50877; GOLOCO; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Phosphoprotein; Reference proteome;
KW Repeat.
FT CHAIN 1..159
FT /note="G-protein-signaling modulator 3"
FT /id="PRO_0000233718"
FT DOMAIN 61..83
FT /note="GoLoco 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00097"
FT DOMAIN 103..125
FT /note="GoLoco 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00097"
FT DOMAIN 131..154
FT /note="GoLoco 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00097"
FT REGION 1..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 77..97
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 34
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y4H4"
FT MOD_RES 38
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319"
FT MOD_RES 55
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319"
FT MOD_RES 58
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319"
FT MOD_RES 61
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y4H4"
FT VAR_SEQ 1..38
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_018153"
FT VAR_SEQ 39..59
FT /note="PPPPGTRHTALGPRSGSLLSL -> MPPLGLGDLLLPPLLLLEPDT (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_018154"
FT CONFLICT 112
FT /note="S -> G (in Ref. 1; BAE33348)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 159 AA; 17619 MW; CEB4FEA5408744F5 CRC64;
MEAERPQEED GEQSLPQDDQ GWPPVNATAR PWRSAPPSPP PPGTRHTALG PRSGSLLSLQ
TELLLDLVAE AQSRRLEEQR ATFHTPEAPP NLAPAPPRLL EDKEQLYSTI LSHQCQRIEA
QRSDPPLPPG GQELLELLLR VQGGGRMEEQ RSRPPTHTC
