GPTC2_HUMAN
ID GPTC2_HUMAN Reviewed; 528 AA.
AC Q9NW75; Q5VYK7; Q5VYK8; Q86YE7;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=G patch domain-containing protein 2;
GN Name=GPATCH2; Synonyms=GPATC2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Embryo;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Lung, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-195, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [6]
RP FUNCTION, INTERACTION WITH DHX15, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=19432882; DOI=10.1111/j.1349-7006.2009.01185.x;
RA Lin M.L., Fukukawa C., Park J.H., Naito K., Kijima K., Shimo A., Ajiro M.,
RA Nishidate T., Nakamura Y., Katagiri T.;
RT "Involvement of G-patch domain containing 2 overexpression in breast
RT carcinogenesis.";
RL Cancer Sci. 100:1443-1450(2009).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-115 AND SER-195, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-115 AND SER-117, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-115 AND SER-117, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146 AND SER-195, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Enhances the ATPase activity of DHX15 in vitro.
CC {ECO:0000269|PubMed:19432882}.
CC -!- SUBUNIT: Interacts with DHX15. {ECO:0000269|PubMed:19432882}.
CC -!- INTERACTION:
CC Q9NW75-2; P68400: CSNK2A1; NbExp=3; IntAct=EBI-12068108, EBI-347804;
CC Q9NW75-2; Q9C005: DPY30; NbExp=3; IntAct=EBI-12068108, EBI-744973;
CC Q9NW75-2; O15481: MAGEB4; NbExp=3; IntAct=EBI-12068108, EBI-751857;
CC Q9NW75-2; Q9NRD5: PICK1; NbExp=4; IntAct=EBI-12068108, EBI-79165;
CC Q9NW75-2; Q99598: TSNAX; NbExp=3; IntAct=EBI-12068108, EBI-742638;
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000269|PubMed:19432882}.
CC Nucleus, nucleolus {ECO:0000269|PubMed:19432882}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9NW75-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9NW75-2; Sequence=VSP_010527, VSP_010528;
CC -!- TISSUE SPECIFICITY: Testis. {ECO:0000269|PubMed:19432882}.
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DR EMBL; AK001114; BAA91509.1; -; mRNA.
DR EMBL; AC096641; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL354659; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471100; EAW93333.1; -; Genomic_DNA.
DR EMBL; BC042193; AAH42193.1; -; mRNA.
DR EMBL; BC063474; AAH63474.1; -; mRNA.
DR CCDS; CCDS1518.1; -. [Q9NW75-1]
DR CCDS; CCDS73031.1; -. [Q9NW75-2]
DR RefSeq; NP_001284683.1; NM_001297754.2. [Q9NW75-2]
DR RefSeq; NP_060510.1; NM_018040.4. [Q9NW75-1]
DR AlphaFoldDB; Q9NW75; -.
DR BioGRID; 120415; 23.
DR IntAct; Q9NW75; 10.
DR STRING; 9606.ENSP00000355902; -.
DR iPTMnet; Q9NW75; -.
DR PhosphoSitePlus; Q9NW75; -.
DR BioMuta; GPATCH2; -.
DR DMDM; 48428171; -.
DR EPD; Q9NW75; -.
DR jPOST; Q9NW75; -.
DR MassIVE; Q9NW75; -.
DR MaxQB; Q9NW75; -.
DR PaxDb; Q9NW75; -.
DR PeptideAtlas; Q9NW75; -.
DR PRIDE; Q9NW75; -.
DR ProteomicsDB; 82908; -. [Q9NW75-1]
DR ProteomicsDB; 82909; -. [Q9NW75-2]
DR Antibodypedia; 20729; 55 antibodies from 13 providers.
DR DNASU; 55105; -.
DR Ensembl; ENST00000366934.3; ENSP00000355901.3; ENSG00000092978.11. [Q9NW75-2]
DR Ensembl; ENST00000366935.8; ENSP00000355902.3; ENSG00000092978.11. [Q9NW75-1]
DR GeneID; 55105; -.
DR KEGG; hsa:55105; -.
DR MANE-Select; ENST00000366935.8; ENSP00000355902.3; NM_018040.5; NP_060510.1.
DR UCSC; uc001hlf.2; human. [Q9NW75-1]
DR CTD; 55105; -.
DR DisGeNET; 55105; -.
DR GeneCards; GPATCH2; -.
DR HGNC; HGNC:25499; GPATCH2.
DR HPA; ENSG00000092978; Low tissue specificity.
DR neXtProt; NX_Q9NW75; -.
DR OpenTargets; ENSG00000092978; -.
DR PharmGKB; PA162390064; -.
DR VEuPathDB; HostDB:ENSG00000092978; -.
DR eggNOG; KOG0154; Eukaryota.
DR GeneTree; ENSGT00410000025698; -.
DR HOGENOM; CLU_041240_2_0_1; -.
DR InParanoid; Q9NW75; -.
DR OMA; DQEMDNN; -.
DR OrthoDB; 606839at2759; -.
DR PhylomeDB; Q9NW75; -.
DR TreeFam; TF331954; -.
DR PathwayCommons; Q9NW75; -.
DR SignaLink; Q9NW75; -.
DR BioGRID-ORCS; 55105; 12 hits in 1079 CRISPR screens.
DR ChiTaRS; GPATCH2; human.
DR GenomeRNAi; 55105; -.
DR Pharos; Q9NW75; Tdark.
DR PRO; PR:Q9NW75; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q9NW75; protein.
DR Bgee; ENSG00000092978; Expressed in buccal mucosa cell and 154 other tissues.
DR Genevisible; Q9NW75; HS.
DR GO; GO:0016607; C:nuclear speck; IDA:HPA.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR InterPro; IPR000467; G_patch_dom.
DR InterPro; IPR026291; Gpatch2.
DR PANTHER; PTHR14195:SF4; PTHR14195:SF4; 1.
DR Pfam; PF01585; G-patch; 1.
DR SMART; SM00443; G_patch; 1.
DR PROSITE; PS50174; G_PATCH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..528
FT /note="G patch domain-containing protein 2"
FT /id="PRO_0000087566"
FT DOMAIN 467..513
FT /note="G-patch"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00092"
FT REGION 36..119
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 232..282
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 487..528
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 60..76
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 95..119
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 235..254
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 255..272
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 513..528
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 115
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19690332,
FT ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692"
FT MOD_RES 117
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231,
FT ECO:0007744|PubMed:21406692"
FT MOD_RES 146
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 195
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:23186163"
FT VAR_SEQ 367..376
FT /note="VPIPGPVGNK -> ATNWTSEIPL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_010527"
FT VAR_SEQ 377..528
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_010528"
FT CONFLICT 220
FT /note="G -> A (in Ref. 4; AAH63474)"
FT /evidence="ECO:0000305"
FT CONFLICT 225
FT /note="D -> N (in Ref. 4; AAH42193)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 528 AA; 58944 MW; 472143144700DC26 CRC64;
MFGAAGRQPI GAPAAGNSWH FSRTMEELVH DLVSALEESS EQARGGFAET GDHSRSISCP
LKRQARKRRG RKRRSYNVHH PWETGHCLSE GSDSSLEEPS KDYRENHNNN KKDHSDSDDQ
MLVAKRRPSS NLNNNVRGKR PLWHESDFAV DNVGNRTLRR RRKVKRMAVD LPQDISNKRT
MTQPPEGCRD QDMDSDRAYQ YQEFTKNKVK KRKLKIIRQG PKIQDEGVVL ESEETNQTNK
DKMECEEQKV SDELMSESDS SSLSSTDAGL FTNDEGRQGD DEQSDWFYEK ESGGACGITG
VVPWWEKEDP TELDKNVPDP VFESILTGSF PLMSHPSRRG FQARLSRLHG MSSKNIKKSG
GTPTSMVPIP GPVGNKRMVH FSPDSHHHDH WFSPGARTEH DQHQLLRDNR AERGHKKNCS
VRTASRQTSM HLGSLCTGDI KRRRKAAPLP GPTTAGFVGE NAQPILENNI GNRMLQNMGW
TPGSGLGRDG KGISEPIQAM QRPKGLGLGF PLPKSTSATT TPNAGKSA
