GPTC4_MOUSE
ID GPTC4_MOUSE Reviewed; 415 AA.
AC Q3TFK5; Q8VCN2; Q9CU32; Q9D3L4;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=G patch domain-containing protein 4;
GN Name=Gpatch4; Synonyms=Gpatc4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, and Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130 AND SER-258, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-258, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3TFK5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3TFK5-2; Sequence=VSP_025482;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB30685.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK018306; BAB31153.1; -; mRNA.
DR EMBL; AK017310; BAB30685.1; ALT_FRAME; mRNA.
DR EMBL; AK169111; BAE40893.1; -; mRNA.
DR EMBL; BC019490; AAH19490.1; -; mRNA.
DR CCDS; CCDS50948.1; -. [Q3TFK5-1]
DR RefSeq; NP_001104279.1; NM_001110809.2. [Q3TFK5-1]
DR RefSeq; NP_001273786.1; NM_001286857.1. [Q3TFK5-1]
DR RefSeq; NP_079939.3; NM_025663.4. [Q3TFK5-1]
DR AlphaFoldDB; Q3TFK5; -.
DR BioGRID; 211596; 1.
DR IntAct; Q3TFK5; 1.
DR STRING; 10090.ENSMUSP00000132699; -.
DR iPTMnet; Q3TFK5; -.
DR PhosphoSitePlus; Q3TFK5; -.
DR EPD; Q3TFK5; -.
DR jPOST; Q3TFK5; -.
DR MaxQB; Q3TFK5; -.
DR PaxDb; Q3TFK5; -.
DR PeptideAtlas; Q3TFK5; -.
DR PRIDE; Q3TFK5; -.
DR ProteomicsDB; 269624; -. [Q3TFK5-1]
DR ProteomicsDB; 269625; -. [Q3TFK5-2]
DR Antibodypedia; 35319; 107 antibodies from 14 providers.
DR Ensembl; ENSMUST00000029707; ENSMUSP00000029707; ENSMUSG00000028069. [Q3TFK5-1]
DR Ensembl; ENSMUST00000166021; ENSMUSP00000132699; ENSMUSG00000028069. [Q3TFK5-1]
DR Ensembl; ENSMUST00000193398; ENSMUSP00000141786; ENSMUSG00000028069. [Q3TFK5-1]
DR GeneID; 66614; -.
DR KEGG; mmu:66614; -.
DR UCSC; uc008pts.3; mouse. [Q3TFK5-1]
DR CTD; 54865; -.
DR MGI; MGI:1913864; Gpatch4.
DR VEuPathDB; HostDB:ENSMUSG00000028069; -.
DR eggNOG; KOG2809; Eukaryota.
DR GeneTree; ENSGT00390000008765; -.
DR HOGENOM; CLU_674312_0_0_1; -.
DR InParanoid; Q3TFK5; -.
DR OMA; ILGKYGW; -.
DR OrthoDB; 1573336at2759; -.
DR PhylomeDB; Q3TFK5; -.
DR TreeFam; TF326721; -.
DR BioGRID-ORCS; 66614; 6 hits in 73 CRISPR screens.
DR ChiTaRS; Gpatch4; mouse.
DR PRO; PR:Q3TFK5; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q3TFK5; protein.
DR Bgee; ENSMUSG00000028069; Expressed in ear vesicle and 249 other tissues.
DR ExpressionAtlas; Q3TFK5; baseline and differential.
DR Genevisible; Q3TFK5; MM.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0002244; P:hematopoietic progenitor cell differentiation; IGI:MGI.
DR GO; GO:0090069; P:regulation of ribosome biogenesis; IBA:GO_Central.
DR InterPro; IPR000467; G_patch_dom.
DR Pfam; PF01585; G-patch; 1.
DR SMART; SM00443; G_patch; 1.
DR PROSITE; PS50174; G_PATCH; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Isopeptide bond; Phosphoprotein;
KW Reference proteome; Ubl conjugation.
FT CHAIN 1..415
FT /note="G patch domain-containing protein 4"
FT /id="PRO_0000287463"
FT DOMAIN 11..57
FT /note="G-patch"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00092"
FT REGION 116..141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 191..415
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 117..131
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 201..237
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 250..268
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 290..309
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 317..354
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 362..376
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q5T3I0"
FT MOD_RES 4
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q5T3I0"
FT MOD_RES 116
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q5T3I0"
FT MOD_RES 130
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355"
FT MOD_RES 258
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT CROSSLNK 46
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q5T3I0"
FT VAR_SEQ 240..326
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025482"
FT CONFLICT 174
FT /note="L -> R (in Ref. 1; BAB30685)"
FT /evidence="ECO:0000305"
FT CONFLICT 184
FT /note="Q -> R (in Ref. 2; AAH19490)"
FT /evidence="ECO:0000305"
FT CONFLICT 317
FT /note="S -> N (in Ref. 1; BAB31153)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 415 AA; 46554 MW; 600A85B147A92B1A CRC64;
MSVTPEVKSR GMKFAEEQLL KHGWTQGKGL GRRENGITQA LKVTLKQDNH GVGHDPAKEF
TDHWWSDLFN KTAASLVVDS GKDGVQIRRL SKETTQRSHP KPSLLYQKFV KTATLTSGEE
KPDRDLGNCS DVDNHEPTPP KILTDEMLLK ACEGRTAHKA ARIGITMKAK LARLEAQEQA
FLAQLKGSKA LGTSQPLTDS EPSQKKKKKR KQKEGEEAAT TEKSLGDELL GHTDRSFRDS
RKKKKRQKAE RQGTAIGSEE EEAAGESGPR ELSTEQSDQP SRKKKKRRKQ RHEEDGEMGV
CDEGGRDVTS RPKAVNSGGD KDPRRSSKKR GTCGEDLDTQ EEEGKDDLTK GERKVRRKDK
RKRQQCCEED LDVSSKDDGG TWVAEDAGER SRQYPKERAK KKKRKRDRGS EVDLS
