GPTC8_MOUSE
ID GPTC8_MOUSE Reviewed; 1505 AA.
AC A2A6A1; Q80TY1;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=G patch domain-containing protein 8;
GN Name=Gpatch8; Synonyms=Gpatc8, Kiaa0553;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 840-1505.
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-915; SER-918 AND SER-1179,
RP AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-479, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
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DR EMBL; AL596258; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK122307; BAC65589.1; -; mRNA.
DR CCDS; CCDS48944.1; -.
DR RefSeq; NP_001152964.1; NM_001159492.1.
DR AlphaFoldDB; A2A6A1; -.
DR SMR; A2A6A1; -.
DR BioGRID; 231931; 7.
DR STRING; 10090.ENSMUSP00000120649; -.
DR iPTMnet; A2A6A1; -.
DR PhosphoSitePlus; A2A6A1; -.
DR EPD; A2A6A1; -.
DR jPOST; A2A6A1; -.
DR MaxQB; A2A6A1; -.
DR PaxDb; A2A6A1; -.
DR PeptideAtlas; A2A6A1; -.
DR PRIDE; A2A6A1; -.
DR ProteomicsDB; 271081; -.
DR Antibodypedia; 52434; 35 antibodies from 14 providers.
DR Ensembl; ENSMUST00000143842; ENSMUSP00000120649; ENSMUSG00000034621.
DR GeneID; 237943; -.
DR KEGG; mmu:237943; -.
DR UCSC; uc007lsb.2; mouse.
DR CTD; 23131; -.
DR MGI; MGI:1918667; Gpatch8.
DR VEuPathDB; HostDB:ENSMUSG00000034621; -.
DR eggNOG; KOG2184; Eukaryota.
DR GeneTree; ENSGT00940000159523; -.
DR HOGENOM; CLU_006418_0_0_1; -.
DR InParanoid; A2A6A1; -.
DR OMA; AEPEYYH; -.
DR OrthoDB; 332880at2759; -.
DR PhylomeDB; A2A6A1; -.
DR TreeFam; TF332138; -.
DR BioGRID-ORCS; 237943; 9 hits in 72 CRISPR screens.
DR ChiTaRS; Gpatch8; mouse.
DR PRO; PR:A2A6A1; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; A2A6A1; protein.
DR Bgee; ENSMUSG00000034621; Expressed in otic placode and 250 other tissues.
DR Genevisible; A2A6A1; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR InterPro; IPR000467; G_patch_dom.
DR InterPro; IPR022755; Znf_C2H2_jaz.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF01585; G-patch; 1.
DR Pfam; PF12171; zf-C2H2_jaz; 1.
DR SMART; SM00443; G_patch; 1.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS50174; G_PATCH; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 1.
PE 1: Evidence at protein level;
KW Acetylation; Coiled coil; Isopeptide bond; Metal-binding; Phosphoprotein;
KW Reference proteome; Ubl conjugation; Zinc; Zinc-finger.
FT CHAIN 1..1505
FT /note="G patch domain-containing protein 8"
FT /id="PRO_0000379799"
FT DOMAIN 40..86
FT /note="G-patch"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00092"
FT ZN_FING 136..160
FT /note="C2H2-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 166..244
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 322..393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 419..537
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 575..1304
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 89..124
FT /evidence="ECO:0000255"
FT COMPBIAS 166..204
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 322..343
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 352..366
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 432..455
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 484..498
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 499..521
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 575..621
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 645..661
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 662..684
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 685..701
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 740..764
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 788..806
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 807..831
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 832..849
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 871..895
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 896..936
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 952..983
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1013..1034
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1048..1062
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1065..1089
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1159..1185
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1290..1304
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 479
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT MOD_RES 648
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UKJ3"
FT MOD_RES 733
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UKJ3"
FT MOD_RES 735
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UKJ3"
FT MOD_RES 753
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UKJ3"
FT MOD_RES 915
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 918
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 985
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UKJ3"
FT MOD_RES 1013
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UKJ3"
FT MOD_RES 1018
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UKJ3"
FT MOD_RES 1037
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UKJ3"
FT MOD_RES 1039
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UKJ3"
FT MOD_RES 1085
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UKJ3"
FT MOD_RES 1179
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CROSSLNK 311
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9UKJ3"
FT CROSSLNK 573
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9UKJ3"
FT CROSSLNK 1109
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9UKJ3"
FT CONFLICT 954
FT /note="T -> I (in Ref. 2; BAC65589)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1505 AA; 164987 MW; 0BCC873DA8AE6DDF CRC64;
MADRFSRFNE DRDFQGNHFD QYEEGHLEIE QASLDKPIES DNIGHRLLQK HGWKLGQGLG
KSLQGRTDPI PIVVKYDVMG MGRMEMELDY AEDATERRRV LEVEKEDTEE LRQKYKDYVD
KEKAIAKALE DLRANFYCEL CDKQYQKHQE FDNHINSYDH AHKQRLKDLK QREFARNVSS
RSRKDEKKQE KALRRLHELA EQRKQAECAP GSGPMFRPTT VAVDEDGGEE DKDESSTNSG
ASAVSSCGFG ADFSTDKGGS FTSVQITNTT GLSQAPGLAS QGISFGIKNN LGPPLQKLGV
SFSFAKKAPV KLESIASVFK DHAEEGSSED GTKADEKSSD QGVQKVGDTD GTGNLDGKKE
DEDPQDGGSL ASTLSKLKRM KREEGTGATE PEYYHYIPPA HCKVKPNFPF LLFMRASEQM
EGDHSAHSKS APENRKSSSP KPQGCSKTAA SPGAERTVSE ASELQKEAAV AGPSEPGGKT
ETKKGSGGGE DEQSVESRET SESPMCESNP KDISQATPAT KAGQGPKHPT GPFFPVLSKD
ESTALQWPSE LLIFTKAEPS ISYSCNPLYF DFKLSRNKDA KAKGTEKPKD VAGSSKDHLQ
SLDPREPNKS QEEEQDVVLS SEGRVDEPAS GAACSSLNKQ EPGGSHMSET EDTGRSHPSK
KEPSGKSHRH KKKKKHKKSS KHKRKHKADT EEKSSKAESG EKSKKRKKRK RKKNKSSAAA
DSERGPKSEP PGSGSPAPPR RRRRAQDDSQ RRSLPAEEGN SGKKDDGGGG SSCQDHSGRK
HKGEPPTSSC QRRANTKHSS RSSHRSQPSS GDEDSDDASS HRLHQKSPSQ YSEEEEEEEE
EEEEEDEDSG SEHSRSRSRS GHRHSSHRSS RRSYSSSSDA SSDQSCYSRQ HSYSDDSYSD
YSDRSRRHSK RSHDSDDSDY TSSKHRSKRH KYSSSDDDYS LSCSQSRSRS RSHTRERSRS
RGRSRSSSCS RSRSKRRSRS TTAHSWQRSR SYSRDRSRST RSPSQRSGSR KGSWGHESPE
ERRSGRRDFI RSKIYRSQSP HYFQSGRGEG PGKKEDGRGD DSKGAGLPSQ NSNTGTGRGS
ESDCSPEDKN SVTARLLLEK IQSRKVERKP NVCEEVLATP NKAGLKYKNP PQGYFGPKLP
PSLGNKPVLP MIGKLPATRK SNKKCEESGL ERGEEQEHSE PEEGSPRSSD APFGHQFSEE
AAGPLSDPPP EEPKSEEATA DHSVAPLGTP AHTDCYPGDP AISHNYLPDP SDGDTLESLD
SGSQPGPVES SLLPIAPDLE HFPNYAPPSG EPSIESTDGT EDASLAPLES QPITFTPEEM
EKYSKLQQAA QQHIQQQLLA KQVKAFPAST ALAPATPALQ PIHIQQPATA SATSITTVQH
AILQHHAAAA AAAIGIHPHP HPQPLAQVHH IPQPHLTPIS LSHLTHSIIP GHPATFLASH
PIHIIPASAI HPGPFTFHPV PHAALYPTLL APRPAAAAAT ALHLHPLLHP IFSGQDLQHP
PSHGT
