GP_PUUMB
ID GP_PUUMB Reviewed; 275 AA.
AC P41264;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Envelope glycoprotein;
DE AltName: Full=M polyprotein;
DE Contains:
DE RecName: Full=Glycoprotein C {ECO:0000250|UniProtKB:P08668};
DE Short=Gc;
DE AltName: Full=Glycoprotein G2;
DE Flags: Fragment;
GN Name=GP;
OS Puumala virus (strain Berkel).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Ellioviricetes; Bunyavirales; Hantaviridae; Mammantavirinae;
OC Orthohantavirus.
OX NCBI_TaxID=38998;
OH NCBI_TaxID=9606; Homo sapiens (Human).
OH NCBI_TaxID=447135; Myodes glareolus (Bank vole) (Clethrionomys glareolus).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=7995985; DOI=10.1093/infdis/170.6.1456;
RA Pilaski J., Feldmann H., Morzunov S.P., Rollin P.E., Ruo S.L., Lauer B.,
RA Peters C.J., Nichol S.T.;
RT "Genetic identification of a new Puumala virus strain causing severe
RT hemorrhagic fever with renal syndrome in Germany.";
RL J. Infect. Dis. 170:1456-1462(1994).
RN [2]
RP REVIEW.
RX PubMed=24755564; DOI=10.3390/v6041801;
RA Cifuentes-Munoz N., Salazar-Quiroz N., Tischler N.D.;
RT "Hantavirus Gn and Gc envelope glycoproteins: key structural units for
RT virus cell entry and virus assembly.";
RL Viruses 6:1801-1822(2014).
CC -!- FUNCTION: [Glycoprotein C]: Forms homotetramers with glycoprotein N at
CC the surface of the virion. Attaches the virion to host cell receptors
CC including integrin ITGAV/ITGB3. This attachment induces virion
CC internalization predominantly through clathrin-dependent endocytosis.
CC Class II fusion protein that promotes fusion of viral membrane with
CC host endosomal membrane after endocytosis of the virion.
CC {ECO:0000250|UniProtKB:P08668}.
CC -!- SUBUNIT: [Glycoprotein C]: Homodimer. Homotetramer; forms
CC heterotetrameric Gn-Gc spikes in the pre-fusion conformation (By
CC similarity). Homotrimer; forms homotrimer in the post-fusion
CC conformation at acidic pH (By similarity). Interacts (via C-terminus)
CC with the nucleoprotein (By similarity). {ECO:0000250|UniProtKB:P08668,
CC ECO:0000250|UniProtKB:P27312, ECO:0000250|UniProtKB:P41266}.
CC -!- SUBCELLULAR LOCATION: [Glycoprotein C]: Virion membrane
CC {ECO:0000250|UniProtKB:P08668}; Single-pass type I membrane protein
CC {ECO:0000305}. Host cell surface {ECO:0000250|UniProtKB:P08668}. Host
CC Golgi apparatus membrane {ECO:0000250|UniProtKB:P08668}; Single-pass
CC type I membrane protein {ECO:0000250|UniProtKB:P08668}. Host
CC endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P08668}; Single-
CC pass type I membrane protein {ECO:0000250|UniProtKB:P08668}.
CC -!- DOMAIN: [Glycoprotein C]: The C-terminus is necessary for proper
CC localization in the Golgi (By similarity). The cytoplasmic tail is
CC involved in binding to the nucleocapsid (By similarity).
CC {ECO:0000250|UniProtKB:P27312}.
CC -!- PTM: [Envelope glycoprotein]: Envelope polyprotein precursor is quickly
CC cleaved in vivo just after synthesis, presumably by host signal
CC peptidase. {ECO:0000250|UniProtKB:P08668}.
CC -!- SIMILARITY: Belongs to the hantavirus envelope glycoprotein family.
CC {ECO:0000305}.
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DR EMBL; L36944; AAA50566.1; -; Genomic_RNA.
DR SMR; P41264; -.
DR GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044228; C:host cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0039654; P:fusion of virus membrane with host endosome membrane; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW.
DR InterPro; IPR002532; Hanta_Gc.
DR Pfam; PF01561; Hanta_G2; 1.
PE 3: Inferred from homology;
KW Disulfide bond; Fusion of virus membrane with host endosomal membrane;
KW Fusion of virus membrane with host membrane; Glycoprotein;
KW Host endoplasmic reticulum; Host Golgi apparatus; Host membrane;
KW Host-virus interaction; Membrane; Phosphoprotein; Repeat;
KW Viral attachment to host cell; Viral penetration into host cytoplasm;
KW Virion; Virus entry into host cell.
FT CHAIN <1..>275
FT /note="Envelope glycoprotein"
FT /id="PRO_0000232519"
FT CHAIN <1..>275
FT /note="Glycoprotein C"
FT /evidence="ECO:0000250"
FT /id="PRO_0000036821"
FT CARBOHYD 122
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000250|UniProtKB:P41266"
FT DISULFID 1..10
FT /evidence="ECO:0000250|UniProtKB:P08668"
FT DISULFID 18..27
FT /evidence="ECO:0000250|UniProtKB:P08668"
FT DISULFID 58..62
FT /evidence="ECO:0000250|UniProtKB:P08668"
FT DISULFID 164..194
FT /evidence="ECO:0000250|UniProtKB:P08668"
FT DISULFID 187..239
FT /evidence="ECO:0000250|UniProtKB:P08668"
FT DISULFID 204..209
FT /evidence="ECO:0000250|UniProtKB:P08668"
FT DISULFID 240..245
FT /evidence="ECO:0000250|UniProtKB:P08668"
FT NON_TER 1
FT NON_TER 275
SQ SEQUENCE 275 AA; 29415 MW; 9FE1077EF357DBA6 CRC64;
CIQLGTEQTC KSVDSNDCLV TTSVKVCLIG TVSKFQPSDT LLFLGPLEQG GLIFKQWCTT
TCQFGDPGDI MSTPVGMKCP ELSGSFRKKC AFATTPVCQF DGNTISGYKR MIATKDSFQS
FNVTEPHISA SSLEWIDPDS SLRDHINVIV GRDLSFQDLS ETPCQVDLTT TSIDGAWGSG
VGFNLICSVS LTECSTFLTS IKACDSAMCY GSTTANLLRG QNTVHIVGKG GHSGSKFMCC
HDTKCSSTGL IAAAPHLDRV TGYNQADSDK IFDDG
