GR02A_DROME
ID GR02A_DROME Reviewed; 428 AA.
AC Q9W594;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 3.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Putative gustatory receptor 2a;
GN Name=Gr2a; ORFNames=CG18531;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [4]
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=22031876; DOI=10.1523/jneurosci.3363-11.2011;
RA Kwon J.Y., Dahanukar A., Weiss L.A., Carlson J.R.;
RT "Molecular and cellular organization of the taste system in the Drosophila
RT larva.";
RL J. Neurosci. 31:15300-15309(2011).
CC -!- FUNCTION: Probable gustatory receptor which mediates acceptance or
CC avoidance behavior, depending on its not yet determined substrates.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=C;
CC IsoId=Q9W594-1; Sequence=Displayed;
CC Name=B;
CC IsoId=Q9W594-2; Sequence=VSP_037656;
CC -!- TISSUE SPECIFICITY: Expressed in neurons of the terminal external
CC chemosensory organ, the dorsal external chemosensory organ, as well as
CC in the dorsal pharyngeal sense organ of larvae.
CC {ECO:0000269|PubMed:22031876}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr2a subfamily. {ECO:0000305}.
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DR EMBL; AE014298; AAF45624.3; -; Genomic_DNA.
DR EMBL; AL035632; CAB38469.1; -; Genomic_DNA.
DR RefSeq; NP_001162633.1; NM_001169162.2. [Q9W594-1]
DR RefSeq; NP_525038.3; NM_080299.4. [Q9W594-2]
DR AlphaFoldDB; Q9W594; -.
DR BioGRID; 57650; 3.
DR DIP; DIP-22899N; -.
DR IntAct; Q9W594; 6.
DR STRING; 7227.FBpp0289461; -.
DR TCDB; 1.A.69.3.4; the heteromeric odorant receptor channel (horc) family.
DR GlyGen; Q9W594; 2 sites.
DR PaxDb; Q9W594; -.
DR DNASU; 31093; -.
DR EnsemblMetazoa; FBtr0114515; FBpp0113007; FBgn0265139. [Q9W594-2]
DR EnsemblMetazoa; FBtr0300224; FBpp0289461; FBgn0265139. [Q9W594-1]
DR GeneID; 31093; -.
DR KEGG; dme:Dmel_CG18531; -.
DR CTD; 31093; -.
DR FlyBase; FBgn0265139; Gr2a.
DR VEuPathDB; VectorBase:FBgn0265139; -.
DR eggNOG; ENOG502T2FZ; Eukaryota.
DR GeneTree; ENSGT00940000166130; -.
DR HOGENOM; CLU_678378_0_0_1; -.
DR InParanoid; Q9W594; -.
DR OMA; AVWSAPH; -.
DR PhylomeDB; Q9W594; -.
DR SignaLink; Q9W594; -.
DR BioGRID-ORCS; 31093; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31093; -.
DR PRO; PR:Q9W594; -.
DR Proteomes; UP000000803; Chromosome X.
DR Genevisible; Q9W594; DM.
DR GO; GO:0030424; C:axon; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004930; F:G protein-coupled receptor activity; ISS:FlyBase.
DR GO; GO:0008527; F:taste receptor activity; ISS:FlyBase.
DR GO; GO:0050912; P:detection of chemical stimulus involved in sensory perception of taste; NAS:UniProtKB.
DR GO; GO:0050909; P:sensory perception of taste; ISS:FlyBase.
DR InterPro; IPR013604; 7TM_chemorcpt.
DR PANTHER; PTHR21143; PTHR21143; 1.
DR Pfam; PF08395; 7tm_7; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..428
FT /note="Putative gustatory receptor 2a"
FT /id="PRO_0000216487"
FT TOPO_DOM 1..40
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 41..61
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 62..145
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 146..166
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 167..173
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 174..194
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 195..250
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 251..271
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 272..300
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 301..321
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 322..349
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 350..370
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 371..395
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 396..416
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 417..428
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT CARBOHYD 195
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 345
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 352..369
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000305"
FT /id="VSP_037656"
SQ SEQUENCE 428 AA; 48492 MW; 31BFD8098BD05472 CRC64;
MDTLRALEPL HRACQVCNLW PWRLAPPPDS EGILLRRSRW LELYGWTVLI AATSFTVYGL
FQESSVEEKQ DSESTISSIG HTVDFIQLVG MRVAHLAALL EALWQRQAQR GFFAELGEID
RLLSKALRVD VEAMRINMRR QTSRRAVWIL WGYAVSQLLI LGAKLLSRGD RFPIYWISYL
LPLLVCGLRY FQIFNATQLV RQRLDVLLVA LQQLQLHQKG PAVDTVLEEQ EDLEEAAMDR
LIAVRLVYQR VWALVALLNR CYGLSMLMQV GNDFLAITSN CYWMFLNFRQ SAASPFDILQ
IVASGVWSAP HLGNVLVLSL LCDRTAQCAS RLALCLHQVS VDLRNESHNA LVGTLVRYCA
PLIILVPLQI TQFSLQLLHQ RLHFSAAGFF NVDCTLLYTI VGATTTYLII LIQFHMSEST
IGSDSNGQ
