GR05A_DROME
ID GR05A_DROME Reviewed; 444 AA.
AC Q9W497; Q68B17; Q68B25; Q68B58; Q68B67; Q68B82; Q68B87; Q68B91; Q68BB7;
AC Q68BC2; Q68BC4; Q68BC6; Q68BD1; Q68BD4; Q68BE7; Q68BE8; Q68BF3; Q95NT7;
AC Q95NX8; Q95NY8; Q95NZ9; Q95P00; Q95YH9; Q95YI0; Q95YI1; Q95YN5; Q95YN6;
AC Q95YN7;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Gustatory receptor 5a for trehalose;
DE AltName: Full=Trehalose receptor;
GN Name=Gr5a; Synonyms=GRLU.7, Tre; ORFNames=CG15779;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, VARIANTS, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=AK07, AK10, AK13, AK17, AK19, AK41, Canton-S, EP(X)496, HG84,
RC Oregon-R, Shanghai, Singapore, Tananarive, w cv, and w cx;
RX PubMed=11566105; DOI=10.1016/s0960-9822(01)00450-x;
RA Ueno K., Ohta M., Morita H., Mikuni Y., Nakajima S., Yamamoto K., Isono K.;
RT "Trehalose sensitivity in Drosophila correlates with mutations in and
RT expression of the gustatory receptor gene Gr5a.";
RL Curr. Biol. 11:1451-1455(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND VARIANT THR-218.
RC STRAIN=KY02-C301, KY02-C302, KY02-C303, KY02-C304, KY02-C305, KY02-C307,
RC KY02-C308, KY02-C309, KY02-C310, KY02-C311, KY02-C312, KY02-C314,
RC KY02-C315, KY02-C316, KY02-C319, KY02-C320, KY02-C323, KY02-C325,
RC KY02-C326, KY02-C327, KY02-C330, KY02-C331, KY02-C332, KY02-C333,
RC KY02-C335, KY02-C336, KY02-C337, KY02-C338, KY02-C341, KY02-C342,
RC KY02-C343, KY02-C344, KY02-C345, KY02-C346, KY02-C347, KY02-C348,
RC KY02-C349, KY02-C350, KY02-C351, KY02-C352, KY02-C353, KY02-C355,
RC KY02-C356, KY02-C358, KY02-C359, KY02-C361, KY02-C362, KY02-C365,
RC KY02-C366, KY02-C367, KY02-C368, KY02-C370, KY02-C372, KY02-C373,
RC KY02-C374, KY02-C376, KY02-C377, KY02-C378, KY02-C379, KY02-C380,
RC KY02-C381, KY02-C382, KY02-C383, KY02-C384, KY02-C387, KY02-C388,
RC KY02-C389, KY02-C390, KY02-C392, KY02-C393, KY02-C394, KY02-C395,
RC KY02-C396, KY02-C397, KY02-C400, KY02-C402, KY02-C404, KY02-C407,
RC KY02-C411, KY02-C412, KY02-C413, KY02-C414, KY02-C417, KY02-C419,
RC KY02-C420, KY02-C421, KY02-C425, KY02-C426, KY02-C428, KY02-C429,
RC KY02-C432, KY02-C433, KY02-C434, KY02-C435, KY02-C436, KY02-C439,
RC KY02-C441, KY02-C443, KY02-C445, KY02-C446, KY02-C447, KY02-C448,
RC KY02-C449, KY02-C451, KY02-C452, KY02-C453, KY02-C454, KY02-C455,
RC KY02-C456, KY02-C457, KY02-C460, KY02-C462, KY02-C463, KY02-C464,
RC KY02-C465, KY02-C467, KY02-C469, KY02-C470, KY02-C476, KY02-C477,
RC KY02-C478, KY02-C479, KY02-C480, KY02-C481, KY02-C483, KY02-C484,
RC KY02-C485, KY02-C486, KY02-C487, KY02-C488, KY02-C489, KY02-C490,
RC KY02-C491, KY02-C492, KY02-C493, KY02-C494, KY02-C496, KY02-C497,
RC KY02-C500, KY02-C504, KY02-C508, KY02-C510, KY02-C511, KY02-C512,
RC KY02-C513, KY02-C514, KY02-C515, KY02-C516, KY02-C517, KY02-C518,
RC KY02-C519, and KY02-C520;
RX PubMed=15342513; DOI=10.1534/genetics.104.027045;
RA Inomata N., Goto H., Itoh M., Isono K.;
RT "A single-amino-acid change of the gustatory receptor gene, Gr5a, has a
RT major effect on trehalose sensitivity in a natural population of Drosophila
RT melanogaster.";
RL Genetics 167:1749-1758(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP IDENTIFICATION.
RX PubMed=10710312; DOI=10.1126/science.287.5459.1830;
RA Clyne P.J., Warr C.G., Carlson J.R.;
RT "Candidate taste receptors in Drosophila.";
RL Science 287:1830-1834(2000).
RN [6]
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11704765; DOI=10.1038/nn765;
RA Dahanukar A., Foster K., van der Goes van Naters W.M., Carlson J.R.;
RT "A Gr receptor is required for response to the sugar trehalose in taste
RT neurons of Drosophila.";
RL Nat. Neurosci. 4:1182-1186(2001).
RN [8]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=14523229; DOI=10.1073/pnas.2135339100;
RA Chyb S., Dahanukar A., Wickens A., Carlson J.R.;
RT "Drosophila Gr5a encodes a taste receptor tuned to trehalose.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:14526-14530(2003).
RN [9]
RP IDENTIFICATION IN THE GUSTATORY RECEPTOR SUBFAMILY.
RX PubMed=14608037; DOI=10.1073/pnas.2335847100;
RA Robertson H.M., Warr C.G., Carlson J.R.;
RT "Molecular evolution of the insect chemoreceptor gene superfamily in
RT Drosophila melanogaster.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:14537-14542(2003).
RN [10]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15202999; DOI=10.1016/j.cub.2004.05.019;
RA Thorne N., Chromey C., Bray S., Amrein H.;
RT "Taste perception and coding in Drosophila.";
RL Curr. Biol. 14:1065-1079(2004).
RN [11]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15738154; DOI=10.1093/chemse/bjh221;
RA Isono K., Morita H., Kohatsu S., Ueno K., Matsubayashi H., Yamamoto M.T.;
RT "Trehalose sensitivity of the gustatory receptor neurons expressing wild-
RT type, mutant and ectopic Gr5a in Drosophila.";
RL Chem. Senses 30:I275-I276(2005).
RN [12]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=16763022; DOI=10.1523/jneurosci.0857-06.2006;
RA Ueno K., Kohatsu S., Clay C., Forte M., Isono K., Kidokoro Y.;
RT "Gsalpha is involved in sugar perception in Drosophila melanogaster.";
RL J. Neurosci. 26:6143-6152(2006).
RN [13]
RP FUNCTION IN ACCEPTANCE BEHAVIOR.
RX PubMed=16423701; DOI=10.1016/j.neuron.2005.11.037;
RA Marella S., Fischler W., Kong P., Asgarian S., Rueckert E., Scott K.;
RT "Imaging taste responses in the fly brain reveals a functional map of taste
RT category and behavior.";
RL Neuron 49:285-295(2006).
RN [14]
RP FUNCTION.
RX PubMed=17988633; DOI=10.1016/j.neuron.2007.10.024;
RA Dahanukar A., Lei Y.T., Kwon J.Y., Carlson J.R.;
RT "Two Gr genes underlie sugar reception in Drosophila.";
RL Neuron 56:503-516(2007).
RN [15]
RP FUNCTION.
RX PubMed=20543015; DOI=10.1093/chemse/bjq055;
RA Kain P., Badsha F., Hussain S.M., Nair A., Hasan G., Rodrigues V.;
RT "Mutants in phospholipid signaling attenuate the behavioral response of
RT adult Drosophila to trehalose.";
RL Chem. Senses 35:663-673(2010).
CC -!- FUNCTION: Gustatory receptor required for response to the sugar
CC trehalose in taste neurons. Gr5a neurons selectively respond to sugars,
CC in contrast to Gr66a cells which respond to bitter compounds. Flies are
CC attracted to sugars and avoid bitter substances, suggesting that Gr5a
CC neuron activity is sufficient to mediate acceptance behavior. Sugar
CC signal transduction occurs through coupling with G-proteins such as
CC Galpha49B and G-salpha60A. {ECO:0000269|PubMed:11566105,
CC ECO:0000269|PubMed:11704765, ECO:0000269|PubMed:14523229,
CC ECO:0000269|PubMed:15202999, ECO:0000269|PubMed:15342513,
CC ECO:0000269|PubMed:15738154, ECO:0000269|PubMed:16423701,
CC ECO:0000269|PubMed:16763022, ECO:0000269|PubMed:17988633,
CC ECO:0000269|PubMed:20543015}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in labellar chemosensory neurons.
CC {ECO:0000269|PubMed:11704765, ECO:0000269|PubMed:14523229,
CC ECO:0000269|PubMed:15202999}.
CC -!- DISRUPTION PHENOTYPE: Flies exhibit trehalose-specific physiological
CC and behavioral defects (reduced response by chemosensory neurons of the
CC labellar taste hairs). {ECO:0000269|PubMed:11566105,
CC ECO:0000269|PubMed:15738154, ECO:0000269|PubMed:16763022}.
CC -!- MISCELLANEOUS: Variant Thr-218 was found to be the ancestral form in
CC D.melanogaster, suggesting that low trehalose sensitivity was an
CC ancestral form with respect to the receptor function.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr5a subfamily. {ECO:0000305}.
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DR EMBL; AB066619; BAB68243.1; -; Genomic_DNA.
DR EMBL; AB066620; BAB68244.1; -; Genomic_DNA.
DR EMBL; AB066621; BAB68245.1; -; Genomic_DNA.
DR EMBL; AB066622; BAB68246.1; -; Genomic_DNA.
DR EMBL; AB066623; BAB68247.1; -; Genomic_DNA.
DR EMBL; AB066624; BAB68248.1; -; Genomic_DNA.
DR EMBL; AB066625; BAB68249.1; -; Genomic_DNA.
DR EMBL; AB066626; BAB68250.1; -; Genomic_DNA.
DR EMBL; AB066627; BAB68251.1; -; Genomic_DNA.
DR EMBL; AB066628; BAB68252.1; -; Genomic_DNA.
DR EMBL; AB066629; BAB68253.1; -; Genomic_DNA.
DR EMBL; AB066630; BAB68254.1; -; Genomic_DNA.
DR EMBL; AB066631; BAB68255.1; -; Genomic_DNA.
DR EMBL; AB066632; BAB68256.1; -; Genomic_DNA.
DR EMBL; AB066633; BAB68257.1; -; Genomic_DNA.
DR EMBL; AB066634; BAB68258.1; -; Genomic_DNA.
DR EMBL; AB066635; BAB68259.1; -; Genomic_DNA.
DR EMBL; AB066636; BAB68260.1; -; Genomic_DNA.
DR EMBL; AB066637; BAB68261.1; -; Genomic_DNA.
DR EMBL; AB066638; BAB68262.1; -; Genomic_DNA.
DR EMBL; AB066639; BAB68263.1; -; Genomic_DNA.
DR EMBL; AB066640; BAB68264.1; -; Genomic_DNA.
DR EMBL; AB066641; BAB68265.1; -; mRNA.
DR EMBL; AB066642; BAB68266.1; -; mRNA.
DR EMBL; AB066643; BAB68267.1; -; mRNA.
DR EMBL; AB162449; BAD42667.1; -; Genomic_DNA.
DR EMBL; AB162450; BAD42668.1; -; Genomic_DNA.
DR EMBL; AB162451; BAD42669.1; -; Genomic_DNA.
DR EMBL; AB162452; BAD42670.1; -; Genomic_DNA.
DR EMBL; AB162453; BAD42671.1; -; Genomic_DNA.
DR EMBL; AB162454; BAD42672.1; -; Genomic_DNA.
DR EMBL; AB162455; BAD42673.1; -; Genomic_DNA.
DR EMBL; AB162456; BAD42674.1; -; Genomic_DNA.
DR EMBL; AB162457; BAD42675.1; -; Genomic_DNA.
DR EMBL; AB162458; BAD42676.1; -; Genomic_DNA.
DR EMBL; AB162459; BAD42677.1; -; Genomic_DNA.
DR EMBL; AB162460; BAD42678.1; -; Genomic_DNA.
DR EMBL; AB162461; BAD42679.1; -; Genomic_DNA.
DR EMBL; AB162462; BAD42680.1; -; Genomic_DNA.
DR EMBL; AB162463; BAD42681.1; -; Genomic_DNA.
DR EMBL; AB162464; BAD42682.1; -; Genomic_DNA.
DR EMBL; AB162465; BAD42683.1; -; Genomic_DNA.
DR EMBL; AB162466; BAD42684.1; -; Genomic_DNA.
DR EMBL; AB162467; BAD42685.1; -; Genomic_DNA.
DR EMBL; AB162468; BAD42686.1; -; Genomic_DNA.
DR EMBL; AB162469; BAD42687.1; -; Genomic_DNA.
DR EMBL; AB162470; BAD42688.1; -; Genomic_DNA.
DR EMBL; AB162471; BAD42689.1; -; Genomic_DNA.
DR EMBL; AB162472; BAD42690.1; -; Genomic_DNA.
DR EMBL; AB162473; BAD42691.1; -; Genomic_DNA.
DR EMBL; AB162474; BAD42692.1; -; Genomic_DNA.
DR EMBL; AB162475; BAD42693.1; -; Genomic_DNA.
DR EMBL; AB162476; BAD42694.1; -; Genomic_DNA.
DR EMBL; AB162477; BAD42695.1; -; Genomic_DNA.
DR EMBL; AB162478; BAD42696.1; -; Genomic_DNA.
DR EMBL; AB162479; BAD42697.1; -; Genomic_DNA.
DR EMBL; AB162480; BAD42698.1; -; Genomic_DNA.
DR EMBL; AB162481; BAD42699.1; -; Genomic_DNA.
DR EMBL; AB162482; BAD42700.1; -; Genomic_DNA.
DR EMBL; AB162483; BAD42701.1; -; Genomic_DNA.
DR EMBL; AB162484; BAD42702.1; -; Genomic_DNA.
DR EMBL; AB162485; BAD42703.1; -; Genomic_DNA.
DR EMBL; AB162486; BAD42704.1; -; Genomic_DNA.
DR EMBL; AB162487; BAD42705.1; -; Genomic_DNA.
DR EMBL; AB162488; BAD42706.1; -; Genomic_DNA.
DR EMBL; AB162489; BAD42707.1; -; Genomic_DNA.
DR EMBL; AB162490; BAD42708.1; -; Genomic_DNA.
DR EMBL; AB162491; BAD42709.1; -; Genomic_DNA.
DR EMBL; AB162492; BAD42710.1; -; Genomic_DNA.
DR EMBL; AB162493; BAD42711.1; -; Genomic_DNA.
DR EMBL; AB162494; BAD42712.1; -; Genomic_DNA.
DR EMBL; AB162495; BAD42713.1; -; Genomic_DNA.
DR EMBL; AB162496; BAD42714.1; -; Genomic_DNA.
DR EMBL; AB162497; BAD42715.1; -; Genomic_DNA.
DR EMBL; AB162498; BAD42716.1; -; Genomic_DNA.
DR EMBL; AB162499; BAD42717.1; -; Genomic_DNA.
DR EMBL; AB162500; BAD42718.1; -; Genomic_DNA.
DR EMBL; AB162501; BAD42719.1; -; Genomic_DNA.
DR EMBL; AB162502; BAD42720.1; -; Genomic_DNA.
DR EMBL; AB162503; BAD42721.1; -; Genomic_DNA.
DR EMBL; AB162504; BAD42722.1; -; Genomic_DNA.
DR EMBL; AB162505; BAD42723.1; -; Genomic_DNA.
DR EMBL; AB162506; BAD42724.1; -; Genomic_DNA.
DR EMBL; AB162507; BAD42725.1; -; Genomic_DNA.
DR EMBL; AB162508; BAD42726.1; -; Genomic_DNA.
DR EMBL; AB162509; BAD42727.1; -; Genomic_DNA.
DR EMBL; AB162510; BAD42728.1; -; Genomic_DNA.
DR EMBL; AB162511; BAD42729.1; -; Genomic_DNA.
DR EMBL; AB162512; BAD42730.1; -; Genomic_DNA.
DR EMBL; AB162513; BAD42731.1; -; Genomic_DNA.
DR EMBL; AB162514; BAD42732.1; -; Genomic_DNA.
DR EMBL; AB162515; BAD42733.1; -; Genomic_DNA.
DR EMBL; AB162516; BAD42734.1; -; Genomic_DNA.
DR EMBL; AB162517; BAD42735.1; -; Genomic_DNA.
DR EMBL; AB162518; BAD42736.1; -; Genomic_DNA.
DR EMBL; AB162519; BAD42737.1; -; Genomic_DNA.
DR EMBL; AB162520; BAD42738.1; -; Genomic_DNA.
DR EMBL; AB162521; BAD42739.1; -; Genomic_DNA.
DR EMBL; AB162522; BAD42740.1; -; Genomic_DNA.
DR EMBL; AB162523; BAD42741.1; -; Genomic_DNA.
DR EMBL; AB162524; BAD42742.1; -; Genomic_DNA.
DR EMBL; AB162525; BAD42743.1; -; Genomic_DNA.
DR EMBL; AB162526; BAD42744.1; -; Genomic_DNA.
DR EMBL; AB162527; BAD42745.1; -; Genomic_DNA.
DR EMBL; AB162528; BAD42746.1; -; Genomic_DNA.
DR EMBL; AB162529; BAD42747.1; -; Genomic_DNA.
DR EMBL; AB162530; BAD42748.1; -; Genomic_DNA.
DR EMBL; AB162531; BAD42749.1; -; Genomic_DNA.
DR EMBL; AB162532; BAD42750.1; -; Genomic_DNA.
DR EMBL; AB162533; BAD42751.1; -; Genomic_DNA.
DR EMBL; AB162534; BAD42752.1; -; Genomic_DNA.
DR EMBL; AB162535; BAD42753.1; -; Genomic_DNA.
DR EMBL; AB162536; BAD42754.1; -; Genomic_DNA.
DR EMBL; AB162537; BAD42755.1; -; Genomic_DNA.
DR EMBL; AB162538; BAD42756.1; -; Genomic_DNA.
DR EMBL; AB162539; BAD42757.1; -; Genomic_DNA.
DR EMBL; AB162540; BAD42758.1; -; Genomic_DNA.
DR EMBL; AB162541; BAD42759.1; -; Genomic_DNA.
DR EMBL; AB162542; BAD42760.1; -; Genomic_DNA.
DR EMBL; AB162543; BAD42761.1; -; Genomic_DNA.
DR EMBL; AB162544; BAD42762.1; -; Genomic_DNA.
DR EMBL; AB162545; BAD42763.1; -; Genomic_DNA.
DR EMBL; AB162546; BAD42764.1; -; Genomic_DNA.
DR EMBL; AB162547; BAD42765.1; -; Genomic_DNA.
DR EMBL; AB162548; BAD42766.1; -; Genomic_DNA.
DR EMBL; AB162549; BAD42767.1; -; Genomic_DNA.
DR EMBL; AB162550; BAD42768.1; -; Genomic_DNA.
DR EMBL; AB162551; BAD42769.1; -; Genomic_DNA.
DR EMBL; AB162552; BAD42770.1; -; Genomic_DNA.
DR EMBL; AB162553; BAD42771.1; -; Genomic_DNA.
DR EMBL; AB162555; BAD42773.1; -; Genomic_DNA.
DR EMBL; AB162554; BAD42772.1; -; Genomic_DNA.
DR EMBL; AB162556; BAD42774.1; -; Genomic_DNA.
DR EMBL; AB162557; BAD42775.1; -; Genomic_DNA.
DR EMBL; AB162558; BAD42776.1; -; Genomic_DNA.
DR EMBL; AB162559; BAD42777.1; -; Genomic_DNA.
DR EMBL; AB162560; BAD42778.1; -; Genomic_DNA.
DR EMBL; AB162561; BAD42779.1; -; Genomic_DNA.
DR EMBL; AB162562; BAD42780.1; -; Genomic_DNA.
DR EMBL; AB162563; BAD42781.1; -; Genomic_DNA.
DR EMBL; AB162564; BAD42782.1; -; Genomic_DNA.
DR EMBL; AB162565; BAD42783.1; -; Genomic_DNA.
DR EMBL; AB162566; BAD42784.1; -; Genomic_DNA.
DR EMBL; AB162567; BAD42785.1; -; Genomic_DNA.
DR EMBL; AB162568; BAD42786.1; -; Genomic_DNA.
DR EMBL; AB162569; BAD42787.1; -; Genomic_DNA.
DR EMBL; AB162570; BAD42788.1; -; Genomic_DNA.
DR EMBL; AB162571; BAD42789.1; -; Genomic_DNA.
DR EMBL; AB162572; BAD42790.1; -; Genomic_DNA.
DR EMBL; AB162573; BAD42791.1; -; Genomic_DNA.
DR EMBL; AB162574; BAD42792.1; -; Genomic_DNA.
DR EMBL; AB162575; BAD42793.1; -; Genomic_DNA.
DR EMBL; AB162576; BAD42794.1; -; Genomic_DNA.
DR EMBL; AB162577; BAD42795.1; -; Genomic_DNA.
DR EMBL; AB162578; BAD42796.1; -; Genomic_DNA.
DR EMBL; AB162579; BAD42797.1; -; Genomic_DNA.
DR EMBL; AB162580; BAD42798.1; -; Genomic_DNA.
DR EMBL; AB162581; BAD42799.1; -; Genomic_DNA.
DR EMBL; AB162582; BAD42800.1; -; Genomic_DNA.
DR EMBL; AB162583; BAD42801.1; -; Genomic_DNA.
DR EMBL; AB162584; BAD42802.1; -; Genomic_DNA.
DR EMBL; AB162585; BAD42803.1; -; Genomic_DNA.
DR EMBL; AB162586; BAD42804.1; -; Genomic_DNA.
DR EMBL; AB162587; BAD42805.1; -; Genomic_DNA.
DR EMBL; AB162588; BAD42806.1; -; Genomic_DNA.
DR EMBL; AB162589; BAD42807.1; -; Genomic_DNA.
DR EMBL; AB162590; BAD42808.1; -; Genomic_DNA.
DR EMBL; AB162591; BAD42809.1; -; Genomic_DNA.
DR EMBL; AB162592; BAD42810.1; -; Genomic_DNA.
DR EMBL; AB162593; BAD42811.1; -; Genomic_DNA.
DR EMBL; AB162594; BAD42812.1; -; Genomic_DNA.
DR EMBL; AB162595; BAD42813.1; -; Genomic_DNA.
DR EMBL; AB162596; BAD42814.1; -; Genomic_DNA.
DR EMBL; AB162597; BAD42815.1; -; Genomic_DNA.
DR EMBL; AB162598; BAD42816.1; -; Genomic_DNA.
DR EMBL; AB162599; BAD42817.1; -; Genomic_DNA.
DR EMBL; AB162600; BAD42818.1; -; Genomic_DNA.
DR EMBL; AE014298; AAF46060.1; -; Genomic_DNA.
DR RefSeq; NP_511050.1; NM_078495.3.
DR AlphaFoldDB; Q9W497; -.
DR SMR; Q9W497; -.
DR BioGRID; 69345; 2.
DR DIP; DIP-22363N; -.
DR IntAct; Q9W497; 1.
DR STRING; 7227.FBpp0070768; -.
DR PaxDb; Q9W497; -.
DR EnsemblMetazoa; FBtr0070802; FBpp0070768; FBgn0003747.
DR GeneID; 44873; -.
DR KEGG; dme:Dmel_CG15779; -.
DR CTD; 44873; -.
DR FlyBase; FBgn0003747; Gr5a.
DR VEuPathDB; VectorBase:FBgn0003747; -.
DR eggNOG; ENOG502QTKP; Eukaryota.
DR GeneTree; ENSGT00530000064347; -.
DR HOGENOM; CLU_043581_0_0_1; -.
DR InParanoid; Q9W497; -.
DR PhylomeDB; Q9W497; -.
DR BioGRID-ORCS; 44873; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 44873; -.
DR PRO; PR:Q9W497; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0003747; Expressed in gustatory organ and 1 other tissue.
DR ExpressionAtlas; Q9W497; differential.
DR Genevisible; Q9W497; DM.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033041; F:sweet taste receptor activity; IMP:FlyBase.
DR GO; GO:0008527; F:taste receptor activity; IMP:UniProtKB.
DR GO; GO:0001582; P:detection of chemical stimulus involved in sensory perception of sweet taste; IMP:UniProtKB.
DR GO; GO:0050912; P:detection of chemical stimulus involved in sensory perception of taste; IDA:FlyBase.
DR GO; GO:0007637; P:proboscis extension reflex; IMP:FlyBase.
DR GO; GO:0010353; P:response to trehalose; IMP:FlyBase.
DR GO; GO:0007606; P:sensory perception of chemical stimulus; IBA:GO_Central.
DR GO; GO:0050916; P:sensory perception of sweet taste; IMP:FlyBase.
DR GO; GO:0050909; P:sensory perception of taste; IDA:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR009318; Gustatory_rcpt.
DR PANTHER; PTHR21421; PTHR21421; 1.
DR Pfam; PF06151; Trehalose_recp; 1.
DR PIRSF; PIRSF038981; GRP; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..444
FT /note="Gustatory receptor 5a for trehalose"
FT /id="PRO_0000216486"
FT TOPO_DOM 1..56
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 57..77
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 78..178
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 179..199
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..214
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 215..235
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..240
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 241..261
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 262..305
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 306..326
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 327..338
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 339..359
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 360..410
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 411..431
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 432..444
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT VARIANT 5
FT /note="K -> N (in strain: KY02-C351 and KY02-C372)"
FT VARIANT 19
FT /note="I -> V (in strain: AK17, Berkeley, Canton-S,
FT EP(X)496, KY02-C304, KY02-C305, KY02-C312, KY02-C341, KY02-
FT C419, KY02-C470 and w cx)"
FT VARIANT 23
FT /note="M -> I (in strain: KY02-C331, KY02-C392 and Oregon-
FT R)"
FT VARIANT 59
FT /note="P -> S (in strain: KY02-C493)"
FT VARIANT 73
FT /note="C -> S (in strain: KY02-C301, KY02-C302, KY02-C308,
FT KY02-C309, KY02-C310, KY02-C311, KY02-C314, KY02-C315,
FT KY02-C319, KY02-C320, KY02-C325, KY02-C327, KY02-C330,
FT KY02-C332, KY02-C335, KY02-C337, KY02-C342, KY02-C343,
FT KY02-C344, KY02-C346, KY02-C347, KY02-C349, KY02-C351,
FT KY02-C352, KY02-C353, KY02-C355, KY02-C358, KY02-C361,
FT KY02-C367, KY02-C368, KY02-C372, KY02-C374, KY02-C376,
FT KY02-C377, KY02-C378, KY02-C379, KY02-C380, KY02-C381,
FT KY02-C382, KY02-C383, KY02-C384, KY02-C388, KY02-C389,
FT KY02-C390, KY02-C393, KY02-C396, KY02-C400, KY02-C402,
FT KY02-C404, KY02-C411, KY02-C412, KY02-C413, KY02-C414,
FT KY02-C420, KY02-C425, KY02-C426, KY02-C429, KY02-C432,
FT KY02-C433, KY02-C434, KY02-C435, KY02-C436, KY02-C439,
FT KY02-C441, KY02-C446, KY02-C447, KY02-C449, KY02-C451,
FT KY02-C453, KY02-C457, KY02-C460, KY02-C462, KY02-C463,
FT KY02-C467, KY02-C469, KY02-C476, KY02-C477, KY02-C480,
FT KY02-C483, KY02-C484, KY02-C485, KY02-C486, KY02-C488,
FT KY02-C490, KY02-C491, KY02-C492, KY02-C493, KY02-C494,
FT KY02-C496, KY02-C497, KY02-C500, KY02-C504, KY02-C508,
FT KY02-C510, KY02-C511, KY02-C514, KY02-C515, KY02-C516,
FT KY02-C517, KY02-C519, KY02-C520 and Singapore)"
FT VARIANT 164
FT /note="T -> A (in strain: KY02-C302, KY02-C316, KY02-C346,
FT KY02-C350, KY02-C351, KY02-C374, KY02-C379, KY02-C380,
FT KY02-C384, KY02-C390, KY02-C396, KY02-C407, KY02-C425,
FT KY02-C428, KY02-C439, KY02-C443, KY02-C456, KY02-C463,
FT KY02-C478, KY02-C493, KY02-C497, KY02-C500, KY02-C504,
FT KY02-C517 and Singapore)"
FT VARIANT 185
FT /note="V -> I (in strain: KY02-C336)"
FT VARIANT 216
FT /note="L -> H (in strain: AK10, KY02-C315, KY02-C335, KY02-
FT C402, KY02-C469, KY02-C516, KY02-C310, KY02-C319, KY02-
FT C320, KY02-C330, KY02-C336, KY02-C342, KY02-C347, KY02-
FT C355, KY02-C358, KY02-C382, KY02-C393, KY02-C404, KY02-
FT C411, KY02-C432, KY02-C435, KY02-C449, KY02-C467, KY02-
FT C483, KY02-C486, KY02-C488, KY02-C492, KY02-C496, KY02-
FT C514, KY02-C519, Tananarive and w cv)"
FT VARIANT 218
FT /note="A -> T (in strain: AK07, AK10, AK13, KY02-C351,
FT KY02-C439, KY02-C510, KY02-C301, KY02-C302, KY02-C303,
FT KY02-C304, KY02-C308, KY02-C310, KY02-C311, KY02-C315,
FT KY02-C316, KY02-C319, KY02-C320, KY02-C325, KY02-C326,
FT KY02-C330, KY02-C331, KY02-C332, KY02-C335, KY02-C336,
FT KY02-C341, KY02-C342, KY02-C346, KY02-C347, KY02-C348,
FT KY02-C349, KY02-C350, KY02-C352, KY02-C355, KY02-C356,
FT KY02-C358, KY02-C359, KY02-C361, KY02-C362, KY02-C367,
FT KY02-C372, KY02-C373, KY02-C374, KY02-C376, KY02-C378,
FT KY02-C380, KY02-C382, KY02-C383, KY02-C384, KY02-C387,
FT KY02-C388, KY02-C390, KY02-C392, KY02-C393, KY02-C394,
FT KY02-C400, KY02-C402, KY02-C404, KY02-C407, KY02-C411,
FT KY02-C412, KY02-C413, KY02-C417, KY02-C419, KY02-C421,
FT KY02-C426, KY02-C428, KY02-C432, KY02-C433, KY02-C434,
FT KY02-C435, KY02-C441, KY02-C443, KY02-C446, KY02-C449,
FT KY02-C451, KY02-C453, KY02-C454, KY02-C456, KY02-C457,
FT KY02-C460, KY02-C462, KY02-C463, KY02-C467, KY02-C469,
FT KY02-C470, KY02-C477, KY02-C478, KY02-C480, KY02-C483,
FT KY02-C484, KY02-C485, KY02-C486, KY02-C488, KY02-C489,
FT KY02-C490, KY02-C492, KY02-C493, KY02-C494, KY02-C496,
FT KY02-C497, KY02-C500, KY02-C504, KY02-C511, KY02-C513,
FT KY02-C514, KY02-C515, KY02-C516, KY02-C517, KY02-C519,
FT KY02-C520, Oregon-R, Singapore, Tananarive and w cv;
FT decreased sensitivity to trehalose)"
FT /evidence="ECO:0000269|PubMed:15342513"
FT VARIANT 248
FT /note="S -> N (in strain: KY02-C316, KY02-C350, KY02-C428,
FT KY02-C443, KY02-C456 and KY02-C478)"
FT VARIANT 277
FT /note="R -> L (in strain: KY02-C304, KY02-C341, KY02-C419
FT and KY02-C470)"
FT VARIANT 333
FT /note="P -> S (in strain: KY02-C326, KY02-C348, KY02-C359,
FT KY02-C362, KY02-C373, KY02-C394, KY02-C417, KY02-C434,
FT KY02-C448, KY02-C451, KY02-C477 and KY02-C513)"
FT CONFLICT 25..36
FT /note="LKNLKSGLEQIR -> MSTFILITFYNP (in Ref. 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 444 AA; 51169 MW; 1CFD82924C0FF97F CRC64;
MRQLKGRNRC NRAVRHLKIQ GKMWLKNLKS GLEQIRESQV RGTRKNFLHD GSFHEAVAPV
LAVAQCFCLM PVCGISAPTY RGLSFNRRSW RFWYSSLYLC STSVDLAFSI RRVAHSVLDV
RSVEPIVFHV SILIASWQFL NLAQLWPGLM RHWAAVERRL PGYTCCLQRA RPARRLKLVA
FVLLVVSLME HLLSIISVVY YDFCPRRSDP VESYLLGASA QLFEVFPYSN WLAWLGKIQN
VLLTFGWSYM DIFLMMLGMG LSEMLARLNR SLEQQVRQPM PEAYWTWSRT LYRSIVELIR
EVDDAVSGIM LISFGSNLYF ICLQLLKSIN TMPSSAHAVY FYFSLLFLLS RSTAVLLFVS
AINDQAREPL RLLRLVPLKG YHPEVFRFAA ELASDQVALT GLKFFNVTRK LFLAMAGTVA
TYELVLIQFH EDKKTWDCSP FNLD
