GR08A_DROME
ID GR08A_DROME Reviewed; 385 AA.
AC Q9W367; Q29QF1;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Gustatory receptor 8a;
GN Name=Gr8a; ORFNames=CG15371;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22279227; DOI=10.1523/jneurosci.4630-11.2012;
RA Lee Y., Kang M.J., Shim J., Cheong C.U., Moon S.J., Montell C.;
RT "Gustatory receptors required for avoiding the insecticide L-canavanine.";
RL J. Neurosci. 32:1429-1435(2012).
CC -!- FUNCTION: Gustatory receptor which mediates acceptance or avoidance
CC behavior, depending on its substrates. Required for sensing and
CC avoiding L-canavanine, a plant-derived insecticide.
CC {ECO:0000269|PubMed:22279227}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Leads to an inability to detect L-canavanine.
CC {ECO:0000269|PubMed:22279227}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr2a subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014298; AAF46468.3; -; Genomic_DNA.
DR EMBL; BT024439; ABC86501.1; -; mRNA.
DR RefSeq; NP_511097.3; NM_078542.6.
DR AlphaFoldDB; Q9W367; -.
DR IntAct; Q9W367; 1.
DR STRING; 7227.FBpp0071246; -.
DR PaxDb; Q9W367; -.
DR DNASU; 31865; -.
DR EnsemblMetazoa; FBtr0071311; FBpp0071246; FBgn0030108.
DR GeneID; 31865; -.
DR KEGG; dme:Dmel_CG15371; -.
DR CTD; 31865; -.
DR FlyBase; FBgn0030108; Gr8a.
DR VEuPathDB; VectorBase:FBgn0030108; -.
DR eggNOG; ENOG502RW0J; Eukaryota.
DR HOGENOM; CLU_708378_0_0_1; -.
DR InParanoid; Q9W367; -.
DR OMA; FQGAFNF; -.
DR OrthoDB; 1181225at2759; -.
DR PhylomeDB; Q9W367; -.
DR BioGRID-ORCS; 31865; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Gr8a; fly.
DR GenomeRNAi; 31865; -.
DR PRO; PR:Q9W367; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0030108; Expressed in mouthpart.
DR ExpressionAtlas; Q9W367; baseline and differential.
DR Genevisible; Q9W367; DM.
DR GO; GO:0030424; C:axon; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008527; F:taste receptor activity; NAS:UniProtKB.
DR GO; GO:0007635; P:chemosensory behavior; IMP:FlyBase.
DR GO; GO:0050912; P:detection of chemical stimulus involved in sensory perception of taste; NAS:UniProtKB.
DR GO; GO:0017085; P:response to insecticide; IMP:FlyBase.
DR GO; GO:1901354; P:response to L-canavanine; IMP:FlyBase.
DR GO; GO:0050909; P:sensory perception of taste; NAS:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR013604; 7TM_chemorcpt.
DR PANTHER; PTHR21143; PTHR21143; 1.
DR Pfam; PF08395; 7tm_7; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..385
FT /note="Gustatory receptor 8a"
FT /id="PRO_0000216488"
FT TOPO_DOM 1..38
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 60..69
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 91..131
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..154
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 176..248
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 270..284
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 306..347
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..372
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 373..385
FT /note="Extracellular"
FT /evidence="ECO:0000255"
SQ SEQUENCE 385 AA; 44484 MW; 0ED98019E80390C0 CRC64;
MSGHLGRVLQ FHLRLYQVLG FHGLPLPGDG NPARTRRRLM AWSLFLLISL SALVLACLFS
GEEFLYRGDM FGCANDALKY VFAELGVLAI YLETLSSQRH LANFWWLHFK LGGQKTGLVS
LRSEFQQFCR YLIFLYAMMA AEVAIHLGLW QFQALTQHML LFWSTYEPLV WLTYLRNLQF
VLHLELLREQ LTGLEREMGL LAEYSRFASE TGRSFPGFES FLRRRLVQKQ RIYSHVYDML
KCFQGAFNFS ILAVLLTINI RIAVDCYFMY YSIYNNVINN DYYLIVPALL EIPAFIYASQ
SCMVVVPRIA HQLHNIVTDS GCCSCPDLSL QIQNFSLQLL HQPIRIDCLG LTILDCSLLT
RMACSVGTYM IYSIQFIPKF SNTYM
