GR22B_DROME
ID GR22B_DROME Reviewed; 386 AA.
AC P84180; Q59E12;
DT 25-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Putative gustatory receptor 22b;
GN Name=Gr22b {ECO:0000312|FlyBase:FBgn0045500}; ORFNames=CG31931;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP IDENTIFICATION.
RC STRAIN=Oregon-R {ECO:0000269|PubMed:14608037};
RX PubMed=14608037; DOI=10.1073/pnas.2335847100;
RA Robertson H.M., Warr C.G., Carlson J.R.;
RT "Molecular evolution of the insect chemoreceptor gene superfamily in
RT Drosophila melanogaster.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:14537-14542(2003).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=15202999; DOI=10.1016/j.cub.2004.05.019;
RA Thorne N., Chromey C., Bray S., Amrein H.;
RT "Taste perception and coding in Drosophila.";
RL Curr. Biol. 14:1065-1079(2004).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=15738152; DOI=10.1093/chemse/bjh219;
RA Thorne N., Bray S., Amrein H.;
RT "Function and expression of the Drosophila gr genes in the perception of
RT sweet, bitter and pheromone compounds.";
RL Chem. Senses 30:I270-I272(2005).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=22031876; DOI=10.1523/jneurosci.3363-11.2011;
RA Kwon J.Y., Dahanukar A., Weiss L.A., Carlson J.R.;
RT "Molecular and cellular organization of the taste system in the Drosophila
RT larva.";
RL J. Neurosci. 31:15300-15309(2011).
CC -!- FUNCTION: Probable gustatory receptor which mediates acceptance or
CC avoidance behavior, depending on its substrates (By similarity). Seems
CC to be involved in the sensing of bitter taste since it is expressed in
CC neurons that mediate sensitivity to bitter compounds. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in taste bristles in the foreleg and
CC labial palps. In larvae, is expressed in neurons of the dorsal and
CC posterior pharyngeal sense organs. Expressed in taste neurons that
CC mediate sensitivity to bitter compounds. {ECO:0000269|PubMed:15202999,
CC ECO:0000269|PubMed:15738152, ECO:0000269|PubMed:22031876}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr22e subfamily. {ECO:0000305}.
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DR EMBL; AE014134; AAX52653.1; -; Genomic_DNA.
DR RefSeq; NP_001014456.1; NM_001014456.1.
DR AlphaFoldDB; P84180; -.
DR BioGRID; 72915; 1.
DR STRING; 7227.FBpp0099546; -.
DR PaxDb; P84180; -.
DR EnsemblMetazoa; FBtr0100188; FBpp0099546; FBgn0045500.
DR GeneID; 117492; -.
DR KEGG; dme:Dmel_CG31931; -.
DR CTD; 117492; -.
DR FlyBase; FBgn0045500; Gr22b.
DR VEuPathDB; VectorBase:FBgn0045500; -.
DR eggNOG; ENOG502T94A; Eukaryota.
DR GeneTree; ENSGT00940000170904; -.
DR HOGENOM; CLU_033758_0_0_1; -.
DR InParanoid; P84180; -.
DR OMA; LCSHRRF; -.
DR OrthoDB; 960844at2759; -.
DR PhylomeDB; P84180; -.
DR BioGRID-ORCS; 117492; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 117492; -.
DR PRO; PR:P84180; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0045500; Expressed in mouthpart.
DR Genevisible; P84180; DM.
DR GO; GO:0030424; C:axon; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; ISM:FlyBase.
DR GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033041; F:sweet taste receptor activity; IBA:GO_Central.
DR GO; GO:0008527; F:taste receptor activity; ISS:FlyBase.
DR GO; GO:0050912; P:detection of chemical stimulus involved in sensory perception of taste; ISS:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR013604; 7TM_chemorcpt.
DR PANTHER; PTHR21143; PTHR21143; 1.
DR Pfam; PF08395; 7tm_7; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..386
FT /note="Putative gustatory receptor 22b"
FT /id="PRO_0000216494"
FT TOPO_DOM 1..48
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 49..69
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 70..89
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 90..110
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..155
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 156..176
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 177..178
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 179..199
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..254
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 255..275
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 276..282
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 283..303
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 304..363
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 364..384
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 385..386
FT /note="Extracellular"
FT /evidence="ECO:0000250"
SQ SEQUENCE 386 AA; 45462 MW; D8FA6AC430E971EB CRC64;
MFGSSREIRP YLARQMLKTT LYGSWLLGIF PFTLDSGKRI RQLRRSRCLT LYGLVLNYFL
IFTLIRLAFE YRKHKLEAFK RNPVLEMINV VIGIINVLSA LIVHFMNFWG SRKVGEICNE
LLILEYQDFE GLNGRNCPNF NCFVIQKCLT ILGQLLSFFT LNFALPGLEF HICLVLLSCL
MEFSLNLNIM HYHVGVLLIY RYVWLINEQL KDLVSQLKLN PETDFSRIHQ FLSLYKRLLE
LNRKLVIAYE YQMTLFIIAQ LSGNIVVIYF LIVYGLSMRT YSIFLVAFPN SLLINIWDFW
LCIAACDLTE KAGDETAIIL KIFSDLEHRD DKLEMSVNEF AWLCSHRKFR FQLCGLFSMN
CRMGFKMIIT TFLYLVYLVQ FDYMNL
