GR22E_DROME
ID GR22E_DROME Reviewed; 389 AA.
AC P58953;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 02-AUG-2002, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Gustatory receptor for bitter taste 22e;
GN Name=Gr22e; ORFNames=CG31936;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=12971900; DOI=10.1016/s0896-6273(03)00542-7;
RA Bray S., Amrein H.;
RT "A putative Drosophila pheromone receptor expressed in male-specific taste
RT neurons is required for efficient courtship.";
RL Neuron 39:1019-1029(2003).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=18067151; DOI=10.1002/cne.21547;
RA Thorne N., Amrein H.;
RT "Atypical expression of Drosophila gustatory receptor genes in sensory and
RT central neurons.";
RL J. Comp. Neurol. 506:548-568(2008).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=22031876; DOI=10.1523/jneurosci.3363-11.2011;
RA Kwon J.Y., Dahanukar A., Weiss L.A., Carlson J.R.;
RT "Molecular and cellular organization of the taste system in the Drosophila
RT larva.";
RL J. Neurosci. 31:15300-15309(2011).
CC -!- FUNCTION: Gustatory receptor which mediates acceptance or avoidance
CC behavior, depending on its substrates. Seems to be involved in the
CC sensing of bitter taste since it is expressed in neurons that mediate
CC sensitivity to bitter compounds which are also avoidance-type taste
CC neurons. {ECO:0000269|PubMed:18067151}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Taste bristles on the labial palp, labral and
CC cibarial sense organs, chemosensory bristles on the leg and anterior
CC wing margin. In larvae, is expressed in neurons of the terminal
CC external chemosensory organ and in the dorsal pharyngeal sense organ.
CC Neurons expressing Gr22e also express Gr66a and correspond to taste
CC neurons that mediate sensitivity to bitter compounds.
CC {ECO:0000269|PubMed:11516643, ECO:0000269|PubMed:12971900,
CC ECO:0000269|PubMed:18067151, ECO:0000269|PubMed:22031876}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr22e subfamily. {ECO:0000305}.
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DR EMBL; AE014134; AAN10462.1; -; Genomic_DNA.
DR RefSeq; NP_722731.1; NM_164438.1.
DR AlphaFoldDB; P58953; -.
DR BioGRID; 72918; 1.
DR IntAct; P58953; 1.
DR STRING; 7227.FBpp0077556; -.
DR GlyGen; P58953; 2 sites.
DR PaxDb; P58953; -.
DR EnsemblMetazoa; FBtr0077888; FBpp0077556; FBgn0045497.
DR GeneID; 117498; -.
DR KEGG; dme:Dmel_CG31936; -.
DR CTD; 117498; -.
DR FlyBase; FBgn0045497; Gr22e.
DR VEuPathDB; VectorBase:FBgn0045497; -.
DR eggNOG; ENOG502T94A; Eukaryota.
DR GeneTree; ENSGT00940000166130; -.
DR HOGENOM; CLU_033758_0_0_1; -.
DR InParanoid; P58953; -.
DR OMA; MGFRMAI; -.
DR OrthoDB; 853458at2759; -.
DR PhylomeDB; P58953; -.
DR BioGRID-ORCS; 117498; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 117498; -.
DR PRO; PR:P58953; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Genevisible; P58953; DM.
DR GO; GO:0030424; C:axon; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033041; F:sweet taste receptor activity; IBA:GO_Central.
DR GO; GO:0008527; F:taste receptor activity; NAS:UniProtKB.
DR GO; GO:0050909; P:sensory perception of taste; NAS:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR013604; 7TM_chemorcpt.
DR PANTHER; PTHR21143; PTHR21143; 1.
DR Pfam; PF08395; 7tm_7; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Receptor; Reference proteome;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..389
FT /note="Gustatory receptor for bitter taste 22e"
FT /id="PRO_0000216497"
FT TOPO_DOM 1..14
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 15..35
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 36..46
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 47..67
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 68..142
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 143..163
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 164..170
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 171..191
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 192..254
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 255..275
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 276..287
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 288..308
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 309..366
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 367..387
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 388..389
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT CARBOHYD 168
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 281
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 389 AA; 45560 MW; 502436D60B411099 CRC64;
MFRPSGSGYR QKWTGLTLKG ALYGSWILGV FPFAYDSWTR TLRRSKWLIA YGFVLNAAFI
LLVVTNDTES ETPLRMEVFH RNALAEQING IHDIQSLSMV SIMLLRSFWK SGDIERTLNE
LEDLQHRYFR NYSLEECISF DRFVLYKGFS VVLELVSMLV LELGMSPNYS AQFFIGLGSL
CLMLLAVLLG ASHFHLAVVF VYRYVWIVNR ELLKLVNKMA IGETVESERM DLLLYLYHRL
LDLGQRLASI YDYQMVMVMV SFLIANVLGI YFFIIYSISL NKSLDFKILV FVQALVINML
DFWLNVEICE LAERTGRQTS TILKLFNDIE NIDEKLERSI TDFALFCSHR RLRFHHCGLF
YVNYEMGFRM AITSFLYLLF LIQFDYWNL
