GR28A_DROME
ID GR28A_DROME Reviewed; 450 AA.
AC Q9VM09;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2002, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Putative gustatory receptor 28a;
GN Name=Gr28a; ORFNames=CG13787;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [4]
RP TISSUE SPECIFICITY, AND FUNCTION.
RX PubMed=18067151; DOI=10.1002/cne.21547;
RA Thorne N., Amrein H.;
RT "Atypical expression of Drosophila gustatory receptor genes in sensory and
RT central neurons.";
RL J. Comp. Neurol. 506:548-568(2008).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=22031876; DOI=10.1523/jneurosci.3363-11.2011;
RA Kwon J.Y., Dahanukar A., Weiss L.A., Carlson J.R.;
RT "Molecular and cellular organization of the taste system in the Drosophila
RT larva.";
RL J. Neurosci. 31:15300-15309(2011).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=22194978; DOI=10.1371/journal.pone.0029022;
RA Park J.H., Kwon J.Y.;
RT "Heterogeneous expression of Drosophila gustatory receptors in
RT enteroendocrine cells.";
RL PLoS ONE 6:E29022-E29022(2011).
CC -!- FUNCTION: Probable gustatory receptor which mediates acceptance or
CC avoidance behavior, depending on its substrates (By similarity).
CC Atypical expression suggests also nongustatory roles in the nervous
CC system and tissues involved in proprioception, hygroreception, and
CC other sensory modalities. It is also possible that it has chemosensory
CC roles in the detection of internal ligands. {ECO:0000250,
CC ECO:0000269|PubMed:18067151}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: In addition to expression in a large number of
CC taste neurons, Gr28a is also expressed in a few nonchemosensory
CC neurons, including the campaniform sensilla of the wing, leg stretch
CC receptors, and multiple dendritic (MD) neurons in the abdomen. In
CC larvea, is expressed in neurons of the terminal external chemosensory
CC organ, the dorsal external chemosensory organ, as well as in the
CC ventral and posterior pharyngeal sense organ.
CC {ECO:0000269|PubMed:18067151, ECO:0000269|PubMed:22031876,
CC ECO:0000269|PubMed:22194978}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr2a subfamily. {ECO:0000305}.
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DR EMBL; AE014134; AAF52519.2; -; Genomic_DNA.
DR RefSeq; NP_523504.2; NM_078780.5.
DR AlphaFoldDB; Q9VM09; -.
DR BioGRID; 72880; 1.
DR STRING; 7227.FBpp0079087; -.
DR GlyGen; Q9VM09; 2 sites.
DR PaxDb; Q9VM09; -.
DR EnsemblMetazoa; FBtr0079463; FBpp0079087; FBgn0041247.
DR GeneID; 117348; -.
DR KEGG; dme:Dmel_CG13787; -.
DR CTD; 117348; -.
DR FlyBase; FBgn0041247; Gr28a.
DR VEuPathDB; VectorBase:FBgn0041247; -.
DR eggNOG; ENOG502S2QD; Eukaryota.
DR GeneTree; ENSGT00940000166130; -.
DR HOGENOM; CLU_029071_0_0_1; -.
DR InParanoid; Q9VM09; -.
DR OMA; TMRAVKQ; -.
DR OrthoDB; 718242at2759; -.
DR PhylomeDB; Q9VM09; -.
DR BioGRID-ORCS; 117348; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 117348; -.
DR PRO; PR:Q9VM09; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Genevisible; Q9VM09; DM.
DR GO; GO:0030424; C:axon; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008527; F:taste receptor activity; NAS:UniProtKB.
DR GO; GO:0050909; P:sensory perception of taste; NAS:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR013604; 7TM_chemorcpt.
DR PANTHER; PTHR21143; PTHR21143; 1.
DR Pfam; PF08395; 7tm_7; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Receptor; Reference proteome;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..450
FT /note="Putative gustatory receptor 28a"
FT /id="PRO_0000216501"
FT TOPO_DOM 1..47
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 48..68
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..87
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 88..108
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109..138
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 139..159
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 160..171
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 172..192
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 193..292
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 293..313
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 314..329
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 330..350
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 351..407
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 408..424
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 425..450
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT CARBOHYD 82
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 438
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 450 AA; 51857 MW; 9FCB2B611D94258F CRC64;
MAFKLWERFS QADNVFQALR PLTFISLLGL APFRLNLNPR KEVQTSKFSF FAGIVHFLFF
VLCFGISVKE GDSIIGYFFQ TNITRFSDGT LRLTGILAMS TIFGFAMFKR QRLVSIIQNN
IVVDEIFVRL GMKLDYRRIL LSSFLISLGM LLFNVIYLCV SYSLLVSATI SPSFVTFTTF
ALPHINISLM VFKFLCTTDL ARSRFSMLNE ILQDILDAHI EQLSALELSP MHSVVNHRRY
SHRLRNLIST PMKRYSVTSV IRLNPEYAIK QVSNIHNLLC DICQTIEEYF TYPLLGIIAI
SFLFILFDDF YILEAILNPK RLDVFEADEF FAFFLMQLIW YIVIIVLIVE GSSRTILHSS
YTAAIVHKIL NITDDPELRD RLFRLSLQLS HRKVLFTAAG LFRLDRTLIF TITGAATCYL
IILIQFRFTH HMDDTSSNST NNLHSIHLGD
