GR28B_DROME
ID GR28B_DROME Reviewed; 470 AA.
AC Q9VM08;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 02-AUG-2002, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Putative gustatory receptor 28b;
GN Name=Gr28b; ORFNames=CG13788;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [4]
RP TISSUE SPECIFICITY, AND FUNCTION.
RX PubMed=18067151; DOI=10.1002/cne.21547;
RA Thorne N., Amrein H.;
RT "Atypical expression of Drosophila gustatory receptor genes in sensory and
RT central neurons.";
RL J. Comp. Neurol. 506:548-568(2008).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=22031876; DOI=10.1523/jneurosci.3363-11.2011;
RA Kwon J.Y., Dahanukar A., Weiss L.A., Carlson J.R.;
RT "Molecular and cellular organization of the taste system in the Drosophila
RT larva.";
RL J. Neurosci. 31:15300-15309(2011).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=22194978; DOI=10.1371/journal.pone.0029022;
RA Park J.H., Kwon J.Y.;
RT "Heterogeneous expression of Drosophila gustatory receptors in
RT enteroendocrine cells.";
RL PLoS ONE 6:E29022-E29022(2011).
CC -!- FUNCTION: Probable gustatory receptor which mediates acceptance or
CC avoidance behavior, depending on its substrates (By similarity).
CC Atypical expression suggests also nongustatory roles in the nervous
CC system and tissues involved in proprioception, hygroreception, and
CC other sensory modalities. It is also possible that it has chemosensory
CC roles in the detection of internal ligands. {ECO:0000250,
CC ECO:0000269|PubMed:18067151}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=C;
CC IsoId=Q9VM08-1; Sequence=Displayed;
CC Name=A;
CC IsoId=Q9VM08-2; Sequence=VSP_002015, VSP_002019;
CC Name=B;
CC IsoId=Q9VM08-3; Sequence=VSP_002017, VSP_002020;
CC Name=D;
CC IsoId=Q9VM08-4; Sequence=VSP_002018, VSP_002021;
CC Name=E;
CC IsoId=Q9VM08-5; Sequence=VSP_002016, VSP_002022;
CC -!- TISSUE SPECIFICITY: Isoforms A and E have taste neuron-specific
CC expression restricted to the labial palps, the internal taste organs in
CC the pharynx, and the legs. In addition to expression in a large number
CC of taste neurons, isoform A is also expressed in a few nonchemosensory
CC neurons, including the campaniform sensilla of the wing, leg stretch
CC receptors, and multiple dendritic neurons in the abdomen. Isoform B is
CC the only receptor not expressed in gustatory receptor neurons in the
CC labellum. We observe expression of this receptor in a single large cell
CC at the base of each maxillary palp, in campaniform sensilla of the
CC wing, and multiple dendritic neurons in the abdomen. Isoform C is
CC expressed by many gustatory receptor neurons in the labial palps, the
CC pharyngeal taste clusters, and taste neurons in the legs. In addition,
CC isoform C expressed in a single cell at the base of the maxillary
CC palps, neurons in the Johnston's organ (JO), campaniform sensilla of
CC the wing, stretch receptors and the femoral chordotonal organ of the
CC legs, and multiple dendritic neurons in the abdomen.Isoform D is
CC expressed in a small number of gustatory receptor neurons in the labial
CC palps, the ventral cibarial sense organ (VCSO), and legs. Atypical
CC expression is observed in three neurons in the arista, campaniform
CC sensilla of the wing, stretch and femoral chordotonal organ receptors
CC in the legs, and multiple dendritic neurons in the abdomen. In larvae,
CC Isoform A is expressed in neurons of the terminal external chemosensory
CC organ and the dorsal external chemosensory organ; and isoform E is
CC expressed in neurons of the terminal external chemosensory organ.
CC {ECO:0000269|PubMed:18067151, ECO:0000269|PubMed:22031876,
CC ECO:0000269|PubMed:22194978}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr66a subfamily. {ECO:0000305}.
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DR EMBL; AE014134; AAF52520.2; -; Genomic_DNA.
DR EMBL; AE014134; AAS64648.1; -; Genomic_DNA.
DR EMBL; AE014134; AAS64649.1; -; Genomic_DNA.
DR EMBL; AE014134; AAS64650.1; -; Genomic_DNA.
DR EMBL; AE014134; AAS64651.1; -; Genomic_DNA.
DR RefSeq; NP_647614.2; NM_139357.4. [Q9VM08-2]
DR RefSeq; NP_995640.1; NM_205918.3. [Q9VM08-5]
DR RefSeq; NP_995641.1; NM_205919.3. [Q9VM08-4]
DR RefSeq; NP_995642.1; NM_205920.3. [Q9VM08-1]
DR RefSeq; NP_995643.1; NM_205921.3. [Q9VM08-3]
DR AlphaFoldDB; Q9VM08; -.
DR BioGRID; 72917; 2.
DR IntAct; Q9VM08; 4.
DR STRING; 7227.FBpp0089145; -.
DR TCDB; 1.A.69.3.3; the heteromeric odorant receptor channel (horc) family.
DR GlyGen; Q9VM08; 3 sites.
DR PaxDb; Q9VM08; -.
DR DNASU; 117496; -.
DR EnsemblMetazoa; FBtr0079458; FBpp0079086; FBgn0045495. [Q9VM08-2]
DR EnsemblMetazoa; FBtr0079459; FBpp0089144; FBgn0045495. [Q9VM08-3]
DR EnsemblMetazoa; FBtr0079460; FBpp0089145; FBgn0045495. [Q9VM08-1]
DR EnsemblMetazoa; FBtr0079461; FBpp0089142; FBgn0045495. [Q9VM08-4]
DR EnsemblMetazoa; FBtr0079462; FBpp0089143; FBgn0045495. [Q9VM08-5]
DR GeneID; 117496; -.
DR KEGG; dme:Dmel_CG13788; -.
DR CTD; 117496; -.
DR FlyBase; FBgn0045495; Gr28b.
DR VEuPathDB; VectorBase:FBgn0045495; -.
DR eggNOG; ENOG502S2QD; Eukaryota.
DR GeneTree; ENSGT00940000166130; -.
DR InParanoid; Q9VM08; -.
DR OMA; HNMTNRT; -.
DR PhylomeDB; Q9VM08; -.
DR SignaLink; Q9VM08; -.
DR BioGRID-ORCS; 117496; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 117496; -.
DR PRO; PR:Q9VM08; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0045495; Expressed in antenna and 3 other tissues.
DR ExpressionAtlas; Q9VM08; differential.
DR Genevisible; Q9VM08; DM.
DR GO; GO:0030424; C:axon; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008527; F:taste receptor activity; ISS:FlyBase.
DR GO; GO:0042742; P:defense response to bacterium; IMP:FlyBase.
DR GO; GO:0007631; P:feeding behavior; IMP:FlyBase.
DR GO; GO:0006955; P:immune response; IMP:FlyBase.
DR GO; GO:0035006; P:melanization defense response; IMP:FlyBase.
DR GO; GO:0046957; P:negative phototaxis; IMP:FlyBase.
DR GO; GO:0007602; P:phototransduction; IDA:FlyBase.
DR GO; GO:0050909; P:sensory perception of taste; ISS:FlyBase.
DR GO; GO:0040040; P:thermosensory behavior; IMP:FlyBase.
DR InterPro; IPR013604; 7TM_chemorcpt.
DR PANTHER; PTHR21143; PTHR21143; 1.
DR Pfam; PF08395; 7tm_7; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..470
FT /note="Putative gustatory receptor 28b"
FT /id="PRO_0000216502"
FT TOPO_DOM 1..76
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 77..97
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 98..119
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 120..140
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 141..175
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 176..196
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..204
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 205..225
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 226..309
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 310..330
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 331..346
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 347..367
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 368..423
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 424..444
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 445..470
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT CARBOHYD 454
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 464
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 467
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..30
FT /note="Missing (in isoform D)"
FT /evidence="ECO:0000305"
FT /id="VSP_002018"
FT VAR_SEQ 1..27
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000305"
FT /id="VSP_002017"
FT VAR_SEQ 1..23
FT /note="Missing (in isoform E)"
FT /evidence="ECO:0000305"
FT /id="VSP_002016"
FT VAR_SEQ 1..18
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000305"
FT /id="VSP_002015"
FT VAR_SEQ 19..240
FT /note="EVTPGLCQPLRRRFRRFVTAKQLYECLRPVFHVTYIHGLTSFYISCDTKTGK
FT KAIKKTIFGYINGIMHIAMFVFAYSLTIYNNCESVASYFFRSRITYFGDLMQIVSGFIG
FT VTVIYLTAFVPNHRLERCLQKFHTMDVQLQTVGVKIMYSKVLRFSYMVLISMFLVNVLF
FT TGGTFSVLYSSEVAPTMALHFTFLIQHTVIAIAIALFSCFTYLVEMRLVMVN -> MIR
FT CGLDIFRGCRGRFRYWLSARDCYDSISLMVAIAFALGITPFLVRRNALGENSLEQSWYG
FT FLNAIFRWLLLAYCYSYINLRNESLIGYFMRNHVSQISTRVHDVGGIIAAVFTFILPLL
FT LRKYFLKSVKNMVQVDTQLERLRSPVNFNTVVGQVVLVILAVVLLDTVLLTTGLVCLAK
FT MEVYASWQLTFIFVYELLAISITICMFCLMTRTVQRRITCLH (in isoform A)"
FT /evidence="ECO:0000305"
FT /id="VSP_002019"
FT VAR_SEQ 24..241
FT /note="LCQPLRRRFRRFVTAKQLYECLRPVFHVTYIHGLTSFYISCDTKTGKKAIKK
FT TIFGYINGIMHIAMFVFAYSLTIYNNCESVASYFFRSRITYFGDLMQIVSGFIGVTVIY
FT LTAFVPNHRLERCLQKFHTMDVQLQTVGVKIMYSKVLRFSYMVLISMFLVNVLFTGGTF
FT SVLYSSEVAPTMALHFTFLIQHTVIAIAIALFSCFTYLVEMRLVMVNK -> MWLLRRS
FT VGKSGNRPHDVYTCYRLTIFMALCLGIVPYYVSISSEGRGKLTSSYIGYINIIIRMAIY
FT MVNSFYGAVNRDTLMSNFFLTDISNVIDALQKINGMLGIFAILLISLLNRKELLKLLAT
FT FDRLETEAFPRVGVAMHQVAANKKMNRLVIILVGSMVAYITCSFLMISLRDTTTFSISA
FT VISFFSPHFIVCAVSFLAGNVMIKLRIYLSALNE (in isoform E)"
FT /evidence="ECO:0000305"
FT /id="VSP_002022"
FT VAR_SEQ 28..241
FT /note="LRRRFRRFVTAKQLYECLRPVFHVTYIHGLTSFYISCDTKTGKKAIKKTIFG
FT YINGIMHIAMFVFAYSLTIYNNCESVASYFFRSRITYFGDLMQIVSGFIGVTVIYLTAF
FT VPNHRLERCLQKFHTMDVQLQTVGVKIMYSKVLRFSYMVLISMFLVNVLFTGGTFSVLY
FT SSEVAPTMALHFTFLIQHTVIAIAIALFSCFTYLVEMRLVMVNK -> MSALRRVRKYF
FT ISSQVYEALRPLFFLTFLYGLTPFHVVRRKMGESYLKMSCFGVFNIFIYICLCGFCYIS
FT SLRQGESIVGYFFRTEISTIGDRLQIFNGLIAGAVIYTSAILKRCKLLGTLTILHSLDT
FT NFSNIGVRVKYSRIFRYSLLVLIFKLLILGVYFVGVFRLLVSLDVTPSFCVCMTFFLQH
FT SVVSIAICLFCVIAFSFERRLSIINQ (in isoform B)"
FT /evidence="ECO:0000305"
FT /id="VSP_002020"
FT VAR_SEQ 31..241
FT /note="RFRRFVTAKQLYECLRPVFHVTYIHGLTSFYISCDTKTGKKAIKKTIFGYIN
FT GIMHIAMFVFAYSLTIYNNCESVASYFFRSRITYFGDLMQIVSGFIGVTVIYLTAFVPN
FT HRLERCLQKFHTMDVQLQTVGVKIMYSKVLRFSYMVLISMFLVNVLFTGGTFSVLYSSE
FT VAPTMALHFTFLIQHTVIAIAIALFSCFTYLVEMRLVMVNK -> MSFYFCEIFKPRDA
FT FGAEQTLLLYTYLLGLTPFRLRGQAGERQFHLSKIGYLNAFLQLSFFSYCFLAALIEQQ
FT SIVGYFFKSEISQMGDSLQKFIGMTGMSILFLCSSIRVRLLIHIWDRISYIDDRFLNLG
FT VCFNYPAIMRLRLLQIFLINGVQLGYLISSNWMLLGNDVRPIYTAIVAFYVPQIFLLSI
FT VMLFNATLHRLWQHFTVLNQ (in isoform D)"
FT /evidence="ECO:0000305"
FT /id="VSP_002021"
SQ SEQUENCE 470 AA; 53598 MW; 485C2FE5B98D2712 CRC64;
MDIEMAKEPV NPTDTPDIEV TPGLCQPLRR RFRRFVTAKQ LYECLRPVFH VTYIHGLTSF
YISCDTKTGK KAIKKTIFGY INGIMHIAMF VFAYSLTIYN NCESVASYFF RSRITYFGDL
MQIVSGFIGV TVIYLTAFVP NHRLERCLQK FHTMDVQLQT VGVKIMYSKV LRFSYMVLIS
MFLVNVLFTG GTFSVLYSSE VAPTMALHFT FLIQHTVIAI AIALFSCFTY LVEMRLVMVN
KVLKNLAHQW DTRSLKAVNQ KQRSLQCLDS FSMYTIVTKD PAEIIQESME IHHLICEAAA
TANKYFTYQL LTIISIAFLI IVFDAYYVLE TLLGKSKRES KFKTVEFVTF FSCQMILYLI
AIISIVEGSN RAIKKSEKTG GIVHSLLNKT KSAEVKEKLQ QFSMQLMHLK INFTAAGLFN
IDRTLYFTIS GALTTYLIIL LQFTSNSPNN GYGNGSSCCE TFNNMTNHTL
