GR32A_DROME
ID GR32A_DROME Reviewed; 461 AA.
AC Q9VKJ7;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 3.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Gustatory and pheromone receptor 32a;
GN Name=Gr32a; Synonyms=GR32D.1; ORFNames=CG14916;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=10710312; DOI=10.1126/science.287.5459.1830;
RA Clyne P.J., Warr C.G., Carlson J.R.;
RT "Candidate taste receptors in Drosophila.";
RL Science 287:1830-1834(2000).
RN [4]
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [5]
RP TISSUE SPECIFICITY, FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18641642; DOI=10.1038/nn.2161;
RA Miyamoto T., Amrein H.;
RT "Suppression of male courtship by a Drosophila pheromone receptor.";
RL Nat. Neurosci. 11:874-876(2008).
RN [6]
RP TISSUE SPECIFICITY, FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20036540; DOI=10.1016/j.cub.2009.11.038;
RA Koganezawa M., Haba D., Matsuo T., Yamamoto D.;
RT "The shaping of male courtship posture by lateralized gustatory inputs to
RT male-specific interneurons.";
RL Curr. Biol. 20:1-8(2010).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20797533; DOI=10.1016/j.neuron.2010.07.006;
RA Lee Y., Kim S.H., Montell C.;
RT "Avoiding DEET through insect gustatory receptors.";
RL Neuron 67:555-561(2010).
RN [8]
RP TISSUE SPECIFICITY.
RX PubMed=22031876; DOI=10.1523/jneurosci.3363-11.2011;
RA Kwon J.Y., Dahanukar A., Weiss L.A., Carlson J.R.;
RT "Molecular and cellular organization of the taste system in the Drosophila
RT larva.";
RL J. Neurosci. 31:15300-15309(2011).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=21516101; DOI=10.1038/nn.2800;
RA Wang L., Han X., Mehren J., Hiroi M., Billeter J.C., Miyamoto T.,
RA Amrein H., Levine J.D., Anderson D.J.;
RT "Hierarchical chemosensory regulation of male-male social interactions in
RT Drosophila.";
RL Nat. Neurosci. 14:757-762(2011).
RN [10]
RP FUNCTION.
RX PubMed=23106154; DOI=10.3109/01677063.2012.729626;
RA Iyengar A., Imoehl J., Ueda A., Nirschl J., Wu C.F.;
RT "Automated quantification of locomotion, social interaction, and mate
RT preference in Drosophila mutants.";
RL J. Neurogenet. 26:306-316(2012).
RN [11]
RP TISSUE SPECIFICITY, FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23810192; DOI=10.1016/j.cell.2013.06.008;
RA Fan P., Manoli D.S., Ahmed O.M., Chen Y., Agarwal N., Kwong S., Cai A.G.,
RA Neitz J., Renslo A., Baker B.S., Shah N.M.;
RT "Genetic and neural mechanisms that inhibit Drosophila from mating with
RT other species.";
RL Cell 154:89-102(2013).
CC -!- FUNCTION: Gustatory receptor which mediates acceptance or avoidance
CC behavior, depending on its substrates. Required for the response to
CC N,N-Diethyl-meta-toluamide (DEET), the most widely used insect
CC repellent worldwide. Functions as a pheromone receptor for a male
CC inhibitory pheromone and promotes male-male aggression and suppresses
CC male-male courtship. Also promotes preferentially virgin females
CC courting over mated females. {ECO:0000269|PubMed:18641642,
CC ECO:0000269|PubMed:20036540, ECO:0000269|PubMed:20797533,
CC ECO:0000269|PubMed:21516101, ECO:0000269|PubMed:23106154,
CC ECO:0000269|PubMed:23810192}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in the adult labellar chemosensory
CC neurons. Expressed in tarsal neurons for male-male courtship
CC suppression. In larvae, is expressed in neurons of the terminal
CC external chemosensory organ, and the dorsal and posterior external
CC chemosensory organs. {ECO:0000269|PubMed:10710312,
CC ECO:0000269|PubMed:18641642, ECO:0000269|PubMed:20036540,
CC ECO:0000269|PubMed:22031876, ECO:0000269|PubMed:23810192}.
CC -!- DISRUPTION PHENOTYPE: Impairs avoiding N,N-Diethyl-meta-toluamide
CC (DEET). Leads to high male courtship toward males and mated females.
CC Also leads to diminished aggression level of males.
CC {ECO:0000269|PubMed:18641642, ECO:0000269|PubMed:20036540,
CC ECO:0000269|PubMed:20797533, ECO:0000269|PubMed:21516101,
CC ECO:0000269|PubMed:23810192}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr21a subfamily. {ECO:0000305}.
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DR EMBL; AE014134; AAF53071.3; -; Genomic_DNA.
DR RefSeq; NP_523543.3; NM_078819.5.
DR AlphaFoldDB; Q9VKJ7; -.
DR BioGRID; 60607; 1.
DR STRING; 7227.FBpp0079787; -.
DR PaxDb; Q9VKJ7; -.
DR EnsemblMetazoa; FBtr0080198; FBpp0079787; FBgn0041246.
DR GeneID; 34545; -.
DR KEGG; dme:Dmel_CG14916; -.
DR CTD; 34545; -.
DR FlyBase; FBgn0041246; Gr32a.
DR VEuPathDB; VectorBase:FBgn0041246; -.
DR eggNOG; ENOG502T08N; Eukaryota.
DR GeneTree; ENSGT00940000166130; -.
DR HOGENOM; CLU_058693_0_0_1; -.
DR InParanoid; Q9VKJ7; -.
DR OMA; SPNILQW; -.
DR OrthoDB; 687132at2759; -.
DR PhylomeDB; Q9VKJ7; -.
DR BioGRID-ORCS; 34545; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34545; -.
DR PRO; PR:Q9VKJ7; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0041246; Expressed in gustatory receptor neuron (Drosophila) and 3 other tissues.
DR Genevisible; Q9VKJ7; DM.
DR GO; GO:0030424; C:axon; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008527; F:taste receptor activity; NAS:UniProtKB.
DR GO; GO:0008049; P:male courtship behavior; IMP:FlyBase.
DR GO; GO:0050913; P:sensory perception of bitter taste; IMP:FlyBase.
DR GO; GO:0050909; P:sensory perception of taste; NAS:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR013604; 7TM_chemorcpt.
DR PANTHER; PTHR21143; PTHR21143; 1.
DR Pfam; PF08395; 7tm_7; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..461
FT /note="Gustatory and pheromone receptor 32a"
FT /id="PRO_0000216503"
FT TOPO_DOM 1..100
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 101..121
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 122..127
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 128..148
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 149..180
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 181..201
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 202..214
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 215..235
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..317
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 318..338
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 339..348
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 349..369
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 370..414
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 415..435
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 436..461
FT /note="Extracellular"
FT /evidence="ECO:0000250"
SQ SEQUENCE 461 AA; 52925 MW; 003441B63F363ED3 CRC64;
MSPNTWVIEM PTQKTRSHPY PRRISPYRPP VLNRDAFSRD APPMPARNHD HPVFEDIRTI
LSVLKASGLM PIYEQVSDYE VGPPTKTNEF YSFFVRGVVH ALTIFNVYSL FTPISAQLFF
SYRETDNVNQ WIELLLCILT YTLTVFVCAH NTTSMLRIMN EILQLDEEVR RQFGANLSQN
FGFLVKFLVG ITACQAYIIV LKIYAVQGEI TPTSYILLAF YGIQNGLTAT YIVFASALLR
IVYIRFHFIN QLLNGYTYGQ QHRRKEGGAR ARRQRGDVNP NVNPALMEHF PEDSLFIYRM
HNKLLRIYKG INDCCNLILV SFLGYSFYTV TTNCYNLFVQ ITGKGMVSPN ILQWCFAWLC
LHVSLLALLS RSCGLTTTEA NATSQILARV YAKSKEYQNI IDKFLTKSIK QEVQFTAYGF
FAIDNSTLFK IFSAVTTYLV ILIQFKQLED SKVEDPVPEQ T
