GR33A_DROME
ID GR33A_DROME Reviewed; 475 AA.
AC Q9VKA5;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 3.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Gustatory and pheromone receptor 33a;
GN Name=Gr33a; ORFNames=CG17213;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20797533; DOI=10.1016/j.neuron.2010.07.006;
RA Lee Y., Kim S.H., Montell C.;
RT "Avoiding DEET through insect gustatory receptors.";
RL Neuron 67:555-561(2010).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=22031876; DOI=10.1523/jneurosci.3363-11.2011;
RA Kwon J.Y., Dahanukar A., Weiss L.A., Carlson J.R.;
RT "Molecular and cellular organization of the taste system in the Drosophila
RT larva.";
RL J. Neurosci. 31:15300-15309(2011).
RN [6]
RP TISSUE SPECIFICITY, FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19765987; DOI=10.1016/j.cub.2009.07.061;
RA Moon S.J., Lee Y., Jiao Y., Montell C.;
RT "A Drosophila gustatory receptor essential for aversive taste and
RT inhibiting male-to-male courtship.";
RL Curr. Biol. 19:1623-1627(2009).
CC -!- FUNCTION: Gustatory receptor which mediates acceptance or avoidance
CC behavior, depending on its substrates. Required for sensing all
CC nonvolatile repulsive chemicals, including tastants, pheromones, and
CC especially N,N-Diethyl-meta-toluamide (DEET), the most widely used
CC insect repellent worldwide. Functions also as a pheromone receptor for
CC a male inhibitory pheromone leading to male-male courtship suppression.
CC {ECO:0000269|PubMed:19765987, ECO:0000269|PubMed:20797533}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed widely in gustatory receptor neurons
CC (GRNs) that respond to aversive chemicals. In larvae, is expressed in
CC neurons of the terminal external chemosensory organ, and the dorsal,
CC ventral and posterior external chemosensory organs.
CC {ECO:0000269|PubMed:19765987, ECO:0000269|PubMed:22031876}.
CC -!- DISRUPTION PHENOTYPE: Impairs avoiding all nonvolatile repellents
CC tested, ranging from quinine to denatonium, lobeline, caffeine, and
CC N,N-Diethyl-meta-toluamide (DEET). Also displays increased male-to-male
CC courtship. {ECO:0000269|PubMed:19765987, ECO:0000269|PubMed:20797533}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr66a subfamily. {ECO:0000305}.
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DR EMBL; AE014134; AAF53173.4; -; Genomic_DNA.
DR RefSeq; NP_525102.4; NM_080363.8.
DR AlphaFoldDB; Q9VKA5; -.
DR STRING; 7227.FBpp0079919; -.
DR PaxDb; Q9VKA5; -.
DR EnsemblMetazoa; FBtr0080337; FBpp0079919; FBgn0032416.
DR GeneID; 34641; -.
DR KEGG; dme:Dmel_CG17213; -.
DR CTD; 34641; -.
DR FlyBase; FBgn0032416; Gr33a.
DR VEuPathDB; VectorBase:FBgn0032416; -.
DR eggNOG; ENOG502TBCF; Eukaryota.
DR HOGENOM; CLU_573997_0_0_1; -.
DR InParanoid; Q9VKA5; -.
DR OMA; YRRKEFF; -.
DR OrthoDB; 806644at2759; -.
DR PhylomeDB; Q9VKA5; -.
DR BioGRID-ORCS; 34641; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34641; -.
DR PRO; PR:Q9VKA5; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032416; Expressed in gustatory receptor neuron (Drosophila) and 1 other tissue.
DR ExpressionAtlas; Q9VKA5; differential.
DR Genevisible; Q9VKA5; DM.
DR GO; GO:0030424; C:axon; IDA:FlyBase.
DR GO; GO:0044297; C:cell body; IDA:FlyBase.
DR GO; GO:0030425; C:dendrite; IDA:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008527; F:taste receptor activity; NAS:UniProtKB.
DR GO; GO:0008049; P:male courtship behavior; IMP:FlyBase.
DR GO; GO:0060179; P:male mating behavior; IMP:FlyBase.
DR GO; GO:0050913; P:sensory perception of bitter taste; IMP:FlyBase.
DR GO; GO:0050909; P:sensory perception of taste; IMP:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR013604; 7TM_chemorcpt.
DR PANTHER; PTHR21143; PTHR21143; 1.
DR Pfam; PF08395; 7tm_7; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..475
FT /note="Gustatory and pheromone receptor 33a"
FT /id="PRO_0000216504"
FT TOPO_DOM 1..34
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 35..55
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 56..68
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 69..89
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 90..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 129..149
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..158
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 159..179
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 180..319
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 320..340
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 341..357
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 358..378
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 379..441
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 442..462
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 463..475
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT REGION 243..281
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 252..267
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 475 AA; 54585 MW; 81921D34308F6CA1 CRC64;
MIQIMNWFSM VIGLIPLNRQ QSETNFILDY AMMCIVPIFY VACYLLINLS HIIGLCLLDS
CNSVCKLSSH LFMHLGAFLY LTITLLSLYR RKEFFQQFDA RLNDIDAVIQ KCQRVAEMDK
VKVTAVKHSV AYHFTWLFLF CVFTFALYYD VRSLYLTFGN LAFIPFMVSS FPYLAGSIIQ
GEFIYHVSVI SQRFEQINML LEKINQEARH RHAPLTVFDI ESEGKKERKT VTPITVMDGR
TTTGFGNENK FAGEMKRQEG QQKNDDDDLD TSNDEDEDDF DYDNATIAEN TGNTSEANLP
DLFKLHDKIL ALSVITNGEF GPQCVPYMAA CFVVSIFGIF LETKVNFIVG GKSRLLDYMT
YLYVIWSFTT MMVAYIVLRL CCNANNHSKQ SAMIVHEIMQ KKPAFMLSND LFYNKMKSFT
LQFLHWEGFF QFNGVGLFAL DYTFIFSTVS AATSYLIVLL QFDMTAILRN EGLMS
