GR43A_DROME
ID GR43A_DROME Reviewed; 427 AA.
AC Q9V4K2; Q4V4B7;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 3.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Gustatory receptor for sugar taste 43a;
GN Name=Gr43a; Synonyms=GR43.1; ORFNames=CG1712;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=10710312; DOI=10.1126/science.287.5459.1830;
RA Clyne P.J., Warr C.G., Carlson J.R.;
RT "Candidate taste receptors in Drosophila.";
RL Science 287:1830-1834(2000).
RN [5]
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=22031876; DOI=10.1523/jneurosci.3363-11.2011;
RA Kwon J.Y., Dahanukar A., Weiss L.A., Carlson J.R.;
RT "Molecular and cellular organization of the taste system in the Drosophila
RT larva.";
RL J. Neurosci. 31:15300-15309(2011).
RN [7]
RP FUNCTION.
RX PubMed=21709218; DOI=10.1073/pnas.1019622108;
RA Sato K., Tanaka K., Touhara K.;
RT "Sugar-regulated cation channel formed by an insect gustatory receptor.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:11680-11685(2011).
RN [8]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=23178127; DOI=10.1016/j.cell.2012.10.024;
RA Miyamoto T., Slone J., Song X., Amrein H.;
RT "A fructose receptor functions as a nutrient sensor in the Drosophila
RT brain.";
RL Cell 151:1113-1125(2012).
RN [9]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=23850280; DOI=10.1016/j.cub.2013.06.028;
RA Mishra D., Miyamoto T., Rezenom Y.H., Broussard A., Yavuz A., Slone J.,
RA Russell D.H., Amrein H.;
RT "The molecular basis of sugar sensing in Drosophila larvae.";
RL Curr. Biol. 23:1466-1471(2013).
CC -!- FUNCTION: Gustatory receptor which mediates acceptance or avoidance
CC behavior, depending on its substrates. Gr43a is the main sugar receptor
CC in larvae. Functions as a narrowly tuned fructose receptor in taste
CC neurons but also as a fructose receptor in the brain. Necessary and
CC sufficient to sense hemolymph fructose and promote feeding in hungry
CC flies but suppress feeding in satiated flies.
CC {ECO:0000269|PubMed:21709218, ECO:0000269|PubMed:23178127,
CC ECO:0000269|PubMed:23850280}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in the adult labellar chemosensory
CC neurons and in the adult head, abdomen, leg and wing. In larvae, is
CC expressed in taste organs, as well as the brain and the
CC gastrointestinal system. {ECO:0000269|PubMed:10710312,
CC ECO:0000269|PubMed:22031876, ECO:0000269|PubMed:23178127,
CC ECO:0000269|PubMed:23850280}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr21a subfamily. {ECO:0000305}.
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DR EMBL; AE013599; AAF59268.3; -; Genomic_DNA.
DR EMBL; BT023089; AAY55505.1; -; mRNA.
DR RefSeq; NP_523650.2; NM_078926.3.
DR AlphaFoldDB; Q9V4K2; -.
DR SMR; Q9V4K2; -.
DR BioGRID; 61536; 1.
DR DIP; DIP-22568N; -.
DR IntAct; Q9V4K2; 1.
DR STRING; 7227.FBpp0110117; -.
DR TCDB; 1.A.69.3.2; the heteromeric odorant receptor channel (horc) family.
DR GlyGen; Q9V4K2; 3 sites.
DR PaxDb; Q9V4K2; -.
DR DNASU; 35655; -.
DR EnsemblMetazoa; FBtr0089014; FBpp0088086; FBgn0041243.
DR GeneID; 35655; -.
DR KEGG; dme:Dmel_CG1712; -.
DR CTD; 35655; -.
DR FlyBase; FBgn0041243; Gr43a.
DR VEuPathDB; VectorBase:FBgn0041243; -.
DR eggNOG; ENOG502S2QD; Eukaryota.
DR HOGENOM; CLU_029071_2_0_1; -.
DR InParanoid; Q9V4K2; -.
DR OMA; TMADNHE; -.
DR PhylomeDB; Q9V4K2; -.
DR BioGRID-ORCS; 35655; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35655; -.
DR PRO; PR:Q9V4K2; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0041243; Expressed in mouthpart and 3 other tissues.
DR ExpressionAtlas; Q9V4K2; differential.
DR Genevisible; Q9V4K2; DM.
DR GO; GO:0030424; C:axon; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0043025; C:neuronal cell body; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033041; F:sweet taste receptor activity; IMP:FlyBase.
DR GO; GO:0008527; F:taste receptor activity; NAS:UniProtKB.
DR GO; GO:0008343; P:adult feeding behavior; IMP:FlyBase.
DR GO; GO:0071332; P:cellular response to fructose stimulus; IMP:FlyBase.
DR GO; GO:0032098; P:regulation of appetite; IMP:FlyBase.
DR GO; GO:0060259; P:regulation of feeding behavior; IMP:FlyBase.
DR GO; GO:0050916; P:sensory perception of sweet taste; IMP:FlyBase.
DR GO; GO:0050909; P:sensory perception of taste; NAS:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR013604; 7TM_chemorcpt.
DR PANTHER; PTHR21143; PTHR21143; 1.
DR Pfam; PF08395; 7tm_7; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Receptor; Reference proteome;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..427
FT /note="Gustatory receptor for sugar taste 43a"
FT /id="PRO_0000216514"
FT TOPO_DOM 1..2
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 3..23
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 24..33
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 34..54
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 55..81
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 82..102
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 103..131
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 132..152
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..171
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 172..192
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 193..295
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 296..316
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 317..402
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 403..423
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 424..427
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT CARBOHYD 103
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 219
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 234
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 427 AA; 47573 MW; 19928FBA374AABAB CRC64;
MEISQPSIGI FYISKVLALA PYATVRNSKG RVEIGRSWLF TVYSATLTVV MVFLTYRGLL
FDANSEIPVR MKSATSKVVT ALDVSVVVMA IVSGVYCGLF SLNDTLELND RLNKIDNTLN
AYNNFRRDRW RALGMAAVSL LAISILVGLD VGTWMRIAQD MNIAQSDTEL NVHWYIPFYS
LYFILTGLQV NIANTAYGLG RRFGRLNRML SSSFLAENNA TSAIKPQKVS TVKNVSVNRP
AMPSALHASL TKLNGETLPS EAAAKNKGLL LKSLADSHES LGKCVHLLSN SFGIAVLFIL
VSCLLHLVAT AYFLFLELLS KRDNGYLWVQ MLWICFHFLR LLMVVEPCHL AARESRKTIQ
IVCEIERKVH EPILAEAVKK FWQQLLVVDA DFSACGLCRV NRTILTSFAS AIATYLVILI
QFQRTNG
