ID   3S332_PSETE             Reviewed;          15 AA.
AC   P59073;
DT   01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-2002, sequence version 1.
DT   23-FEB-2022, entry version 51.
DE   RecName: Full=Short neurotoxin N2;
DE   AltName: Full=Alpha-neurotoxin;
DE   Flags: Fragment;
OS   Pseudonaja textilis (Eastern brown snake).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Acanthophiinae; Pseudonaja.
OX   NCBI_TaxID=8673;
RN   [1]
RP   PROTEIN SEQUENCE, TOXIC DOSE, MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=10518793; DOI=10.1046/j.1432-1327.1999.00800.x;
RA   Gong N.L., Armugam A., Jeyaseelan K.;
RT   "Postsynaptic short-chain neurotoxins from Pseudonaja textilis: cDNA
RT   cloning, expression and protein characterization.";
RL   Eur. J. Biochem. 265:982-989(1999).
CC   -!- FUNCTION: Binds with high affinity to muscular (alpha-1/CHRNA1) and
CC       neuronal (alpha-7/CHRNA7) nicotinic acetylcholine receptor (nAChR) and
CC       hinders acetylcholine binding to the receptor, thereby impairing
CC       neuromuscular and neuronal transmission. {ECO:0000250|UniProtKB:P60615,
CC       ECO:0000269|PubMed:10518793}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10518793}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=6345; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:10518793};
CC   -!- TOXIC DOSE: LD(50) is 0.80 mg/kg by intravenous injection.
CC       {ECO:0000269|PubMed:10518793}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Type III alpha-neurotoxin sub-subfamily. {ECO:0000305}.
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DR   Proteomes; UP000472273; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW   Ion channel impairing toxin; Neurotoxin; Postsynaptic neurotoxin;
KW   Reference proteome; Secreted; Toxin.
FT   CHAIN           1..>15
FT                   /note="Short neurotoxin N2"
FT                   /evidence="ECO:0000269|PubMed:10518793"
FT                   /id="PRO_0000093617"
FT   UNSURE          3
FT   UNSURE          13
FT   NON_TER         15
SQ   SEQUENCE   15 AA;  1727 MW;  E149FD4BFD1EF0DD CRC64;
     LTCYKGYHDT VVCKP